Mystery peptide with high endotoxin results
- Thread starter Habibibi
- Start date
Mr.Tired
GLP-1 Apprentice
Yikes. Hope they opt to release the name, that's alarming.
Calm Logic
GLP-1 Specialist
Bastards.
tendency
GLP-1 Enthusiast
Gemini is telling me that standard filtering will not remove endotoxins?
Correct
Calm Logic
GLP-1 Specialist
How many in the group are going to trash it?
I would. Vendors don't typically refund or reship based on endontoxins, AFAIK. I've seen a few NAD+ samples test >100 EU/vial lately, but nothing over 1000. I saw Peter M discussing this today, but it was a private test and the vendor and peptide wasn't mentioned.
Calm Logic
GLP-1 Specialist
Yeah, I see Uther's NAD 500 mg was 984 EU/vial in a 3P test.
You need special charged filters for endotoxin. They run about $20 each. Most endos are handled by your natural defenses, and there is a calculation to determine what your body can handle based on time (like how often it's exposed) and weight. It's not usually a problem unless you have an immune condition, or until we get up into some of the values we're starting to see recently. I never used to worry about it, but I'm glad people are testing for it more often.
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Calm Logic
GLP-1 Specialist
Does someone sell them individually? Normally, you have to buy 10, it seems.
$288.30 for a pack of 10:
But, ideally, you want 10 for a whole kit (though you could filter multiple vials at once, I suppose). So cheaper to just throw the trash away.
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tendency
GLP-1 Enthusiast
Shoot, I just ran a bunch of Uther NAD+ .. no issues.
Ok that's good to hear I was wondering how concerned I should be about all this. Thanks for the charged filter idea, I haven't heard of people doing that in practice?
Calm Logic
GLP-1 Specialist
Probably due to cost. The charged endo filter can cost more than a vial of the peptide.
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Yes, for a kit. I did see them individually last year, but at $20 each, I decided I'd take my chances. Not surprised they're more now.
Well that testing group should name the vendor so people don't buy that junk.
GLP1s are negatively charged. These are positively charged filters. They will filter out endotoxins along with the peptide with them.
Calm Logic
GLP-1 Specialist
So the mystery peptide is a GLP! Haha.
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littledragon25
Testing Coordinator
Let's not spread lies.
We have not ‘decided to keep it private’.
It is one result of a few tests on the same pep and we haven’t had the other results yet. The people who paid for the test haven’t had the full picture and so it isn’t ready for release yet. This result has been with us for less than 48 hours and we are doing due diligence to check it before releasing. That is the responsible thing to do. We also issued a warning to put a hold on using this batch (including naming the vendor) until we could verify results.
The test lead himself posted it in STG to ask Peter M to clarify as it is an estimate, which is a change to Endotoxin testing as this appears to now need to be tested at different dilutions for accurate results not estimates. We do not know how accurate the result is as Peter himself has said it needs to be tested at the right dilution.
We have 2 other vials on the way to verify the result at different dilutions.
We aren’t in the business of releasing unverified results and causing mass panic.
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oldrunnerguy
GLP-1 Apprentice
On a Telegram forum for one of the vendors who are on this forum, I was ridiculed for sending out my peptides for endotoxins testing. "What a waste of money" I was told. Well, it was worth it to me.
It's good information, but the question is what do you do with that information. If you have a vial test for <100 EU, you can still use it. If it's >100 EU, that's higher, but your vendor won't reship or refund based on endotoxin testing. So, if then it becomes: how often will I be using this >100 EU peptide (exposure)? If it's daily, you'd probably be fine. Multiple time a day, you would need to do the calculation. Weekly use, you definitely would be OK. Now with the test above of >1000 EU in one vial, that's over the top and Peter M. says it's the highest EU he's ever recorded. I haven't seen if the test was verified, but that kit is a toss and the question becomes: how much else is out there for this vendor and what is it?
Lovedog100
GLP-1 Apprentice
I had to Google endotoxin to understand what you are talking about. As a bacteria, would BAC water be strong enough to kill it?
Endotoxins are lipopolysaccharides (LPS) forming the outer membrane of Gram-negative bacteria (e.g., E. coli, Salmonella), acting as a structural, protective barrier. Released upon bacterial lysis, they cause fever, septic shock, inflammation, and sepsis by inducing massive immune responses, specifically via Lipid A.
Key Aspects of Endotoxin:
Endotoxins are lipopolysaccharides (LPS) forming the outer membrane of Gram-negative bacteria (e.g., E. coli, Salmonella), acting as a structural, protective barrier. Released upon bacterial lysis, they cause fever, septic shock, inflammation, and sepsis by inducing massive immune responses, specifically via Lipid A.
Key Aspects of Endotoxin:
- Location: Found in the outer membrane of Gram-negative bacteria.
- Functions: Provides structural integrity, protects against host defenses, and maintains bacterial viability.
- Structure: Composed of three parts: Lipid A (toxic component), core polysaccharide, and O-antigen.
- Immune Response: Triggers innate immunity by activating macrophages to release inflammatory mediators like TNFα.
- Diseases/Symptoms: Causes severe fever, inflammation, septic shock, and diarrhea.
- Detection: Tested using Limulus Amebocyte Lysate (LAL) assays, which use horseshoe crab blood to detect minute amounts.
Bac water doesn't kill them
CNCCurrency
GLP-1 Specialist
Your body can handle 5iu per kilo per hour.
Endotoxins are fragments of bacterial cell walls, not living organisms, because they are already 'dead,' they cannot be killed by bacteriostatic water, which in any case does not kill live bacteria but merely inhibits their growth; the threat endotoxins pose is not infection but rather a potentially severe immune reaction that can occur even when no live bacteria are present.
At this point, I think all we know is that the EUs are above 100 EUs, but agree, the estimate is probably low if it works the same way as other Jano tests have worked. I was in a group that the EU<10 test reported an EU value of around 11, with the comment that the value was outside the assay's validated range. On the EU>10 test the results indicated that EUs were around 78. So, this 1,350.5 could be substantially higher (if it works the same way).
agussie
GLP-1 Apprentice
they were my next test buy but after the whole batch number mixup/mislabel thing, i think i'll let some of you guys bleed off their supply. I've also noticed a lack of even vendor coa's from them on some stuff lately.
I haven't seen any mention on STG that the retest for >1000 EU was completed. How did you confirm this?
Someone posted the Jano Test in another TG group
Mr.Tired
GLP-1 Apprentice
Which peptide? I've got a lot of JP stuff and would like to know what to put off using until we have more information.
