Formulas for Reconstituting Peptides

jmon1977

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Dec 4, 2025
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Hey everyone,
I’ve noticed a lot of questions about how to reconstitute peptides, especially around how much Bac water to use and how to calculate doses. I know there are several online calculators, but I like having a single place to view all of my dosages for different peptides (and doses for titration, etc) so I’ve created a little formula guide in spreadsheet form. You can easily replicate it yourself. Here’s how it’s laid out:

Input Fields (Row 2):
  • Peptide Name (A2): Just the name of the peptide.
  • Amount in Vial (mg) (B2): How many milligrams are in the vial.
  • Amount of Bac Water (ml) (C2): How many milliliters of bac water you add.
  • Desired Dose (mg) (D2): The dose you want to draw for each injection.

Calculated Fields:
  • Concentration per ml (E2): This is calculated as =B2/C2. For example, if you have 10 mg in 1 ml, that’s 10 mg/ml.
  • Injection Volume (F2): This is your desired dose divided by the concentration, so =D2/E2.
  • Units in Syringe (G2): If you’re using a 100-unit insulin syringe, this is just =F2*100 to convert the volume into units.

Example:
If you have a peptide vial with 10 mg (B2 = 10) and you add 1 ml of bac water (C2 = 1), and you want a 1 mg dose (D2 = 1), the concentration is =10/1 = 10 mg/ml. For a 1 mg dose, you’ll draw =1/10 = 0.1 ml, which is 10 units on the syringe.

I hope this helps!
 

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