Filtering and contamination.

Calm Logic said:
At least HGH is a low dose. Some people will do 400+ mg of glutathione at a time. I limit myself to 200 mg per injection since that is the IM dose at the local clinic. But now that I think about it again, will do no more than 100 mg per injection.
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Correction: Greater endo risk with low-dose HGH due to at least some HGH being grown in E. coli, apparently, as opposed to yeast. Will definitely try my tesa next instead.
 
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Calm Logic said:
What kills me are people who filter but don't use gloves. Staph is everywhere, and washing them only does so much due to oils in the skin, etc. So I use gloves anytime I draw from a vial. I get nitrile gloves when they are on sale at Harbor Freight for like $6 for a box of 100. And they even have XL sizes.
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I've always just applied a thick squirt of 90% hand sanitizer and thoroughly rub in, that should take care of the staphies, no?
 
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Calm Logic said:
It helps but is not as sterile as using gloves. And 70 percent is better than 90.
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Well, you're gonna love this one this for a point-counterpoint: my buddy takes his morning crap, washes his hands (at least he uses Dial!), and then mixes/injects on his dirty countertop next to the toilet.

Has had 0 issues in 10+ years of doing it this way 😀
 
I don't think Big Pharma tells GLP users to use gloves either, for administration using vials/syringes. Just "wash your hands with soap and water":


But for recon at least, I would use gloves (ideally medical grade, not from my favorite hardware store, haha). The guy from Peptide Test has a recon video where he wears gloves and sprays down his metal surfaces with a spray bottle of 70 percent rubbing alcohol:

View: https://www.youtube.com/watch?v=9YBj26vpHs0

He also filters in the video, using the filters he sells. But his setup is much better than most since he does do lab testing of peptides.

Personally, I would filter if I started using pens. (I bought a number of generic pens and will probably use them for other things, with a dispenser instead of a needle.)

Attached are the written instructions by Peptide Test, similar to their video.
 

Attachments

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Calm Logic said:
I don't think Big Pharma tells GLP users to use gloves either, for administration using vials/syringes. Just "wash your hands with soap and water":


But for recon at least, I would use gloves (ideally medical grade, not from my favorite hardware store, haha). The guy from Peptide Test has a recon video where he wears gloves and sprays down his metal surfaces with a spray bottle of 70 percent rubbing alcohol:

View: https://www.youtube.com/watch?v=9YBj26vpHs0

He also filters in the video, using the filters he sells. But his setup is much better than most since he does do lab testing of peptides.

Personally, I would filter if I started using pens. (I bought a number of generic pens and will probably use them for other things, with a dispenser instead of a needle.)

Attached are the written instructions by Peptide Test, similar to their video.
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I'm going to filter, but I do like your suggestion about gloves and the should be here tomorrow with a couple other supplies. I'll save that Reconstitution procedure too.
 
deluge said:
Now this is interesting, I've seen dozens of people recommend "pre-wetting" the filters, and yet exactly none of the videos I've watched, (also dozens!) does anyone actually pre-wet their filter. The concept seems sound, as does flushing. Does anyone actually do it? Would you mind describing your technique?
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yes I do. It seems to help the flow, and I dont like wasting peps or filters.
 
tendency said:
I've always just applied a thick squirt of 90% hand sanitizer and thoroughly rub in, that should take care of the staphies, no?
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Other than 70 percent being better than 90 percent, your method does seem good for quickly drawing and injecting, but I don't like alcohol drying my Arizona desert hands, haha.
 
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I remember in med scool, we did some studies on gloves.
Touching e.coli and then transferring to petri dish and grow.
With gloves and without gloves.
Guess what. E.coli showed up on both. But less from gloves of course.
It was quite disgusting, lol
Gloves are not 100 % safe, but yes I use it too. it does make a barrier for less contamination.
 
joseblo said:
I'll mention that once you're filtering, you might as well pre-wet and rinse through the filter too. That makes the loss a lot smaller and the volume more accurate even for the 13mm. Heck, I rinse my recon vial, if I'm reconning more than 1ml, just to make sure I get it all!

Start just like the video, with ~20-30U BAC and put a sterile needle on the filter.
Push until it spurts 💦 and go to an even set of units. Insert into cart to push rest.
Disconnect the syringe from the filter (leaving the needle in) cuz yer filtering!
Put a vent on the pressurized cart (it could have some BAC in it too) to equalize.
Some people put a filter on there too, but if it's positive pressure I don't see why.
Pull your reconned pep from the vial and filter with 100U low dead vol syringe.
You'll need a luerlock syringe, but I prefer small ones to the 3mm biggies 🍆.
Get another 20-30U of BAC (whatever you need to get the right final recon vol), and use that to flush the filter. You could use the same to rinse the recon vial.

I'd recommend a longer needle to filter in with the cart vertical, and a short barely penetrating needle to vent (I've even been known to use a 29G pen needle👀 to vent air). That keeps you from blowing bubbles out the top (or the plunger out the back! Heck, I recommend a longer needle to pull the recon too so you can get to the bottom and don't have to fiddle with the stopper.

I've got so many unused 100U insulin syringes, I recon and rinse with a few of those since they're accurate to ~ unit. If the vial has vacuum, you don't have to vent it first. Then finish venting it once it's got at least 1ml and is fully dissolved with the long (~1.5" 25G) barely in the vial so it doesn't blow bubbles, and use that to pull for filtering when pushed all the way to bottom. It helps to spin the vial and rotate the needle so the tip opening is at the bottom.

YMMV!
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wow - i feel like a dumb ass - I have been researching peptides for 2 + years and I have never filtered. Of course there is a part of me that wonders is this a way to get you to spend more money? Don't come at me, lol. Do you seriously do this with every vial?
 
Aterna13 said:
wow - i feel like a dumb ass - I have been researching peptides for 2 + years and I have never filtered. Of course there is a part of me that wonders is this a way to get you to spend more money? Don't come at me, lol. Do you seriously do this with every vial?
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Yes.
I don't get ISRs anymore. The cost is a few dollars and time. A systemic infection, an ER visit, how much will that cost you?
 
desinr-gal said:
Yes.
I don't get ISRs anymore. The cost is a few dollars and time. A systemic infection, an ER visit, how much will that cost you?
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Yeah, the cost is maybe a couple of $/cart and pennies per shot.
Compared even to the grey market contraband it's cheap, and I'd rather not get infected with whatever bacteria happens to survive in south China water right now.
 
BNLFL said:
I do now, never did. Just bought a box of 100 Biologix 13mm filters.

What's ISR please?
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Yes that red raised irritation that hurts and often itches. Never happens to me anymore.
Now, as I understand it, that happens because a body has antibodies to a substance (like bacteria) and so reacts to it. Your body can also develop antibodies to any substance you
introduce to it, even after periods of time when it was okay with that substance. Sudden allergies to bee stings or sea food for example are well documented.
So it's no guarantee that an anti body won't develop. It just eliminates the issue with things that can be filtered out.
 
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I always wipe each one with alcohol. 0.22um PES filters i have ,Also believe that it will not be 100% sterile 😏
 
francesca said:
I always wipe each one with alcohol. 0.22um PES filters i have ,Also believe that it will not be 100% sterile 😏
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Also, make sure, if you buy filters for sterility that they are individually wrapped. Amazon and AliEx sell buckets of filters, but if they aren't sealed in a sterile environment, they won't be clean when you filter through them.
 
joseblo said:
Also, make sure, if you buy filters for sterility that they are individually wrapped. Amazon and AliEx sell buckets of filters, but if they aren't sealed in a sterile environment, they won't be clean when you filter through them.
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I have a box of 100 Biologix 13mm due here tomorrow, all individual wrapped.
 
tendency said:
Well, you're gonna love this one this for a point-counterpoint: my buddy takes his morning crap, washes his hands (at least he uses Dial!), and then mixes/injects on his dirty countertop next to the toilet.

Has had 0 issues in 10+ years of doing it this way 😀
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Your buddy? Sure…😉
 
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