Nexaph T30 Failed Sterility (T30 pre sale Christmas batch)

[BNLFL]

Yeah pre-sale just popped on my email, no Reta yet.

 

[Habibibi]

Hopefully this thread has helped people determine their risk tolerance. I think many people here have found out they could benefit from sticking to compounding, or brand for peace of mind.

 

[Grogu]

Hopefully this thread has helped people determine their risk tolerance. I think many people here have found out they could benefit from sticking to compounding, or brand for peace of mind.

I'm kinda hoping the thread has helped to inspire folks to filter if that was something they weren't already doing. I'm not sure compounded peptides don't suffer the same issues as grey. Brand is the ultimately "peace of mind" but comes with a price.

 

[Neurogroot]

I'm kinda hoping the thread has helped to inspire folks to filter if that was something they weren't already doing. I'm not sure compounded peptides don't suffer the same issues as grey. Brand is the ultimately "peace of mind" but comes with a price.

I’m pretty new to the community. Honestly, I hadn’t ever thought to filter. Mostly because I wasn’t aware that filtering was a thing! Definitely looking at the filters recommended in this thread.

I’ve got a silly question though, if these filters are able to snag yeast or mold, is there any risk to the actual peptide being filtered out? I feel a bit ridiculous asking, but I guess I’m struggling to compare yeast or mold with a peptide. Thanks in advance, and thank you for all the helpful insight!

 

[Calm Logic]

The filtering is based on size. Peptides are smaller than bacteria, etc:

Structure	Typical Size	Filter Status (0.22 micron)	Why?
Mold Spores	2,000 – 10,000 nm	NOT FILTERED	Much larger than the 220 nm pores.
Yeast Cells	3,000 – 5,000 nm	NOT FILTERED	Significantly larger than the pores.
Bacteria	500 – 2,000 nm	NOT FILTERED	Filter is designed to trap these.
Large Viruses	100 – 300 nm	PARTIAL	Some caught; many pass through.
Small Viruses	20 – 100 nm	PASSES	Small enough to sail through.
Endotoxins	10 – 50 nm	PASSES	Too small for 0.22 micron filters.
Peptides	1 – 3 nm	PASSES	Tiny compared to 220 nm opening.

 

[Grogu]

I’m pretty new to the community. Honestly, I hadn’t ever thought to filter. Mostly because I wasn’t aware that filtering was a thing! Definitely looking at the filters recommended in this thread.

I’ve got a silly question though, if these filters are able to snag yeast or mold, is there any risk to the actual peptide being filtered out? I feel a bit ridiculous asking, but I guess I’m struggling to compare yeast or mold with a peptide. Thanks in advance, and thank you for all the helpful insight!

0.22 µm (220 nm) is the pore size of a standard sterilization filter. That size sits just to the right of the flu virus (~100 nm) on this scale, meaning viruses can pass through a 0.22 µm filter while bacteria (~1 µm and larger) cannot. Particulate will also be filtered.

Peptides are usually in the 3 to 5nm range like lipids, so extremely small and not filtered out using a 0.22 µm filter.

 

[neo6488]

Just picked up two kits on the pre-sale. Might pick up 3 more. This batch will likely be the most tested batch in this company's history. They know they need to step their game back up. I am here for it. Sometimes the best time to fish is after the storm.

I'm of the same thought. Two kits of 60 will move me up to a 2 year supply. Revisit sourcing more in a year or so.

 

[agussie]

That's absurd. Compounding pharmacies are actually regulated (badly, but still).

I just finished a vial of tirz from Vios and I’m pretty sure it was contaminated. Green boogies and felt like a 5 week sinus infection. Body pain something fierce. 2 weeks in on a grey kit and all symptoms have pretty well cleared. I question some of the compounders for sure.

 

[Neurogroot]

0.22 µm (220 nm) is the pore size of a standard sterilization filter. That size sits just to the right of the flu virus (~100 nm) on this scale, meaning viruses can pass through a 0.22 µm filter while bacteria (~1 µm and larger) cannot. Particulate will also be filtered.

Peptides are usually in the 3 to 5nm range like lipids, so extremely small and not filtered out using a 0.22 µm filter.

View attachment 18122

This was a perfect explanation. I really appreciate this. Looking forward to reading more of your posts throughout the forums.

 

[Neurogroot]

The filtering is based on size. Peptides are smaller than bacteria, etc:

Just saw this, also a great explanation! I really appreciate the information, and having access to educational content in different formats.

In fact, I already have a “To-Do” task for this week to compare the Biologix filters vs. other reputable brands. I imagine I’ll need a lot as I pin 5 times a week when off-cycle for a chunk of my stack, and an embarrassing 23 times a week at my peak schedule. Yuck.

 

[Grogu]

This was a perfect explanation. I really appreciate this. Looking forward to reading more of your posts throughout the forums.

Glad it was helpful. Sometimes it’s nice to see things visually. Not sure if you’ve already seen this, but this is a popular video tutorial on filtering. Good luck on your journey!

View: https://youtu.be/9YBj26vpHs0?si=vgxiFIAW7W6t4QPW

 

[staffn1]

I wonder if compounding pharmacies filter when they reconstitute for their various custom concentrations of glp's?

 

[Grogu]

I wonder if compounding pharmacies filter when they reconstitute for their various custom concentrations of glp's?

A lot depends on the type of compounding facility, with 503b outsourcing facilities having much more robust protocols. Under FDA oversight, 503b facilities must comply with cGMP (current good manufacturing practices), the same that apply to pharmaceutical manufacturers. This would include filtering and also testing on finished products. 503a pharmacies should be filtering but verification and documentation requirements are much less compared to 503b facilities.

 

[Neurogroot]

Glad it was helpful. Sometimes it’s nice to see things visually. Not sure if you’ve already seen this, but this is a popular video tutorial on filtering. Good luck on your journey!

View: https://youtu.be/9YBj26vpHs0?si=vgxiFIAW7W6t4QPW

I was going to look for a tutorial before buying, but you gave me a great one here. I found it strangely intriguing, the 15 minutes went by real fast. Another home run recommendation, thanks!

The guy mentioned that some people vent, some don’t. Is there any pros or cons to not venting?

 

[Grogu]

I was going to look for a tutorial before buying, but you gave me a great one here. I found it strangely intriguing, the 15 minutes went by real fast. Another home run recommendation, thanks!

The guy mentioned that some people vent, some don’t. Is there any pros or cons to not venting?

The main pro of venting is that it equalizes pressure and allows smooth flow. Without venting, air trapped in the receiving vial creates positive pressure that resists incoming liquid which can slow or even stall the filtration. The lower pressure because of venting also reduces filter mebrane stress. I wonder sometimes when people report blowouts or leaking in the process if it's because they didn't vent. The biggest con to me would be the risk of contamination. The video by Peptide Test shows putting a filter on the venting needle. I'm not sure if that's really necessary, but if it makes someone feel better the filters are very inexpensive.

 

[Calm Logic]

Is anyone talking about filtering it twice, to feel safer?

There was a recent Alice TFC T40 batch that failed sterility and the group buy vendor offered refunds to buyers, but also put the ~70 vials she had up for sale for $85 and they sold out in a day. The vendor fully disclosed the test results, so there is a market for these despite the sterility testing.

Crazy. Even with filtering, I would use as backup to a backup. So may never be used, except for a peptide apocalypse.

 

[Grogu]

Crazy. If I had vials that I knew were infected, I would use as backup to a backup. So may never be used, except for a peptide apocalypse.

Yeah, I think it would be hard for me to pin from a batch that I knew failed sterility, nevermind intentionally buy kits that I knew failed sterility just for a discount. Once you know, you know.

 

[Calm Logic]

Immune compromised? Trash.

Weirded out? Trash.

Souvenir for life? On display in the freezer.

Mitigation strategies, including shortened use window mentioned by @Grogu:

Method	Effectiveness against Staph	Effectiveness against Endotoxins	Risk / Trade-off
Discarding	100% (Absolute)	100% (Absolute)	The only 0% risk option.
0.22µm Filter	High (Physically removes bacteria)	None (Toxins pass through)	High; requires specialized gear and can lose product in the filter.
Smaller Doses	Moderate (Reduces bacterial load)	Moderate (Reduces toxin load)	Reduces systemic impact but doesn't eliminate the local infection risk.
Shortened Use Window	Moderate (Limits colony growth)	None	Requires discarding product much sooner than the standard 28-30 days.
Double Skin Prep	Moderate (Prevents new Staph)	None	Prevents adding skin-surface Staph to already suspect internal Staph.
Bacteriostatic Rest	Low/Moderate (Inhibits growth)	None	Requires 24+ hours of dwell time in 0.9% Benzyl Alcohol.
Freezing Lyophilized	Low/Moderate (Halts metabolism)	None	Only "pauses" replication in the powder; does not sterilize it.

Definition of "Rest": This refers to the incubation period required for the 0.9% benzyl alcohol in bacteriostatic water to effectively disrupt bacterial cell membranes and halt protein synthesis. A minimum of 24 hours is recommended to ensure the preservative has stabilized the solution, though it is not a substitute for true sterilization.

 

[BNLFL]

Immune compromised? Trash.

Weirded out? Trash.

Souvenir for life? On display in the freezer.

Mitigation strategies, including shortened use window mentioned by @Grogu:

Makes the vial collection look more impressive I guess.

 

[Grogu]

Mitigation strategies, including shortened use window mentioned by @Grogu:

Yes, if someone is using this batch (or any batch that fails sterility) it wouldn’t be one of those times to keep it in the fridge for 4 months 😂. Bac can only do so much.

But the bac rest method is an interesting concept and nothing I ever thought about, but makes perfect sense. Let the bac do its thing for 24 hours before pinning. That might be a strategy to use with batches of unknown sterility, along with filtering.

 

[chewonmysac]

The T60 Batch from the January sale (NXP-2P6001152026-01)test results were released publicly by a 3rd party (Lawless) a few days ago, and they all came back Aces. Mass and purity, Endo, Sterility, Heavy metals, Illicit substances. Wish all testing were this thorough, but I get the cost.
These were my first multi-kit purchases, and I was pretty nervous. Picked up four on the March 28th pre-sale and joined the testing group. I guess this is my new Hobby.

 

[deluge]

But the bac rest method is an interesting concept and nothing I ever thought about, but makes perfect sense. Let the bac do its thing for 24 hours before pinning. That might be a strategy to use with batches of unknown sterility, along with filtering.

I reconstitute my peptides days to weeks in advance already (don't we all, when reconstituting lyophilized powder with BAC?).

It would be an interesting test, though; take a vial that fails due to staph, and have it reconstituted, allow to sit, and re-test?

My understanding is BAC doesn't sterilize (not concentrated enough), but prevents microbial growth.

This CDC article seems to indicate it won't work:

Chemical Disinfectants

Chemical disinfectant recommendations for Guideline for Disinfection and Sterilization in Healthcare

www.cdc.gov

 

[neo6488]

The T60 Batch from the January sale (NXP-2P6001152026-01)test results were released publicly by a 3rd party (Lawless) a few days ago, and they all came back Aces. Mass and purity, Endo, Sterility, Heavy metals, Illicit substances. Wish all testing were this thorough, but I get the cost.
These were my first multi-kit purchases, and I was pretty nervous. Picked up four on the March 28th pre-sale and joined the testing group. I guess this is my new Hobby.

Im currently in a testing group for the next t30 release and we are north of 100 people. Full testing should be like $12 a person. This may be the new normal with Nexaph.

 

[Calm Logic]

My understanding is BAC doesn't sterilize (not concentrated enough), but prevents microbial growth.

Right, the opposite of using a petri dish:

another Bacteriostatic Sterile Water thread

maybe I should add to prior bac water threads, but I noticed they veered off into other directions so I decided to start another. Firstly, where are the best places to buy bacteriostatic sterile water (bac water)? Amscomedical.com, peptidetest.com, or hcgsupplies.com, or private sellers...

glp1forum.com

This study is older, but it deliberatedly innoculated vials with bacteria, yet despite this, of 928 samples, only 0.4% of samples demonstrated microbial growth seen, showing the efficacy of 0.9% benzyl alcohol: https://pubmed.ncbi.nlm.nih.gov/6800255/

 

[Grogu]

Im currently in a testing group for the next t30 release and we are north of 100 people. Full testing should be like $12 a person. This may be the new normal with Nexaph.

In the past, large test groups for Nexaph's popular products were typically, so nothing new here. I've seen groups in the 100 people range before. It's definitely more difficult organzing larger groups for their other products (trust me I've tried), but if the presale involves T30, T60, R24 or R48 you're in good shape. There is testing for other products, just much smaller groups.

The issue is that even with these inexpensive test groups, what do you do with the results. My understanding is that Cain isn't doing anything about the tainted T30 batch. Keep in mind that many many many prior 3p test groups tested for sterility and none failed recently except for this batch. So, it's great that this test group will test everything under the sun, but if there is no recourse beyond mass/purity then why pay inflated prices? Might was well chase the lowest price and filter.

In my post history here on glp-1, I've written very positively about Nexaph, especially the ease of order, US Warehouse, use of credit cards, etc. But I hadn't gone down the rabbit hole enough at that point. I thought Nexaph's presale prices weren't terrible, but not so much anymore.

 

[neo6488]

In the past, large test groups for Nexaph's popular products were typically, so nothing new here. I've seen groups in the 100 people range before. It's definitely more difficult organzing larger groups for their other products (trust me I've tried), but if the presale involves T30, T60, R24 or R48 you're in good shape. There is testing for other products, just much smaller groups.

The issue is that even with these inexpensive test groups, what do you do with the results. My understanding is that Cain isn't doing anything about the tainted T30 batch. Keep in mind that many many many prior 3p test groups tested for sterility and none failed recently except for this batch. So, it's great that this test group will test everything under the sun, but if there is no recourse beyond mass/purity then why pay inflated prices? Might was well chase the lowest price and filter.

In my post history here on glp-1, I've written very positively about Nexaph, especially the ease of order, US Warehouse, use of credit cards, etc. But I hadn't gone down the rabbit hole enough at that point. I thought Nexaph's presale prices weren't terrible, but not so much anymore.

I just don't get the math. As an individual researcher it just feels so much more difficult to branch out into other gray. I mean even at inflated prices from nexaph I can get enough for like $400 a year. If I did my own testing it would be multiple years to break even.
That's not even discussing if they truly fucked over the community there is actual recourse with paying via credit cards.
Now if you are buying for multiple people the math changes quickly, but taking significant more risk for a modest savings doesn't seem worth it to me.

 

[Grogu]

I just don't get the math. As an individual researcher it just feels so much more difficult to branch out into other gray. I mean even at inflated prices from nexaph I can get enough for like $400 a year. If I did my own testing it would be multiple years to break even.
That's not even discussing if they truly fucked over the community there is actual recourse with paying via credit cards.
Now if you are buying for multiple people the math changes quickly, but taking significant more risk for a modest savings doesn't seem worth it to me.

The Nexaph test groups are not the only game in town, so you don't have to do the research on your own. A lot of people are involved with group buys that include testing.

Keep in mind, even the Nexaph shills that populate TG don't get all their peptides from Nexaph and are involved in group buys. We may even have a future Nexaph shill auditioning in this thread.

 

[CandyCap]

I just don't get the math. As an individual researcher it just feels so much more difficult to branch out into other gray. I mean even at inflated prices from nexaph I can get enough for like $400 a year. If I did my own testing it would be multiple years to break even.
That's not even discussing if they truly fucked over the community there is actual recourse with paying via credit cards.
Now if you are buying for multiple people the math changes quickly, but taking significant more risk for a modest savings doesn't seem worth it to me.

Yet, here I sit unable to use a very expensive kit, despite being vendor tested. It tested with 3 different varieties of Staph in it. With no recourse.

 

[zpped]

That's not even discussing if they truly fucked over the community there is actual recourse with paying via credit cards.

Good luck with that. You have no recourse.

Now if you are buying for multiple people the math changes quickly, but taking significant more risk for a modest savings doesn't seem worth it to me.

What additional risk are you taking?

 

[BNLFL]

Good luck with that. You have no recourse.

Dispute the purchase/charge? Good luck with that. Especially with what you're buying.