So I got a kit of NAD+ but don't really know how to use it. I honestly don't even know if it's buffered or not, or what buffered means. I watched some videos and feel like I'll recon with 5ml but not sure on dosing or timing and a lot of conflicting info on dr.google. Help, please.
how to NAD+
- Thread starter ambot88
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Fostradamus
GLP-1 Apprentice
I followed this and have titrated up to 100mg every other day.
Pokemon999
GLP-1 Novice
🚫No Source Discussion🚫
Im doing 40mg a day at the moment. Did you notice a difference at 100mg?
catsincali
GLP-1 Apprentice
ty for sharing this!!!
leeann82
GLP-1 Apprentice
Saving this. Nad will be on my next order.
SolidChick
GLP-1 Apprentice
I currently do about 50-75 mg a day.
Itsjacob
Recently Joined
🚫No Source Discussion🚫
SolidChick
GLP-1 Apprentice
Yes I started 25mg a day then increased to 50mg per the dosing chart. I didn’t feel anything so I increased to 75mg.
I still don’t feel anything. I’ll be adding motsc soon.
much appreciated!
Loveandpeaceandjoy
GLP-1 Apprentice
RS followed this protocol initially. Currently at 80mg 3x weekly. Has really boosted weight loss. Will add 5AM soon. Tried Mots but allergic.
bonelifts
GLP-1 Apprentice
Currently wife and I dose 40mg 4 on / 1 off that matches our workout routine. It’s included in our preworkout stack Mots-C, 5-Amino, NAD
Calm Logic
GLP-1 Specialist
MOTS-c does seems to be the worst for triggering an unhappy reaction ("your immune system trying to...quarantine the "danger molecules" you just introduced").
Skidude
Illegitimi non carborundum
I did the NAD+ with the full MITO mix: ss-31/MOTSc/NAD+
I did 100 mg MWF
for the term: see below
I did take TMG to replenish Methyl
Practical Recommendations for NAD+ & TMG Supplementation
- Timing and Dosage
Based on current knowledge, TMG’s role as a long-acting methyl donor means it can be taken at any time of the day. There’s no evidence to suggest that TMG must be taken at the same time as NAD+. A typical dosage is 1,000mg of TMG daily, which should be more than enough to support methylation needs. However, for individuals who find daily TMG supplementation burdensome, taking it only on the days they use their NAD+ patch is also a viable option. - Daily vs. Occasional Supplementation
While some patients prefer to take TMG daily, others opt to only take it when using their NAD+ patches. Both methods are acceptable, and individual responses can vary based on personal methylation capacity. - Methylation Testing
Unfortunately, there aren’t yet precise tests to measure methylation efficiency or NAD+ metabolism in a way that would inform personalized TMG dosing. However, genetic testing for MTHFR mutations and measuring homocysteine levels can provide insight into your methylation capacity.
The Takeaway
While the precise timing and the amount of TMG patients should use with NAD+ are not set in stone, our approach is based on what seems to work for most people: either take TMG daily or on the days when you use NAD+ supplements, especially if you have known methylation issues. You can easily find TMG supplements at online retailers or most vitamin and supplement shops.At Sellers website, we’ll continue to monitor the latest research and provide updated guidance as more is understood about the interplay between NAD+ and methyl donors like TMG.
Taking Trimethylglycine (TMG) alongside NAD+ precursors (like NMN or NR) helps replenish methyl groups, which are depleted during the metabolic process of raising NAD+ levels. This combination supports optimal methylation, crucial for DNA repair, detoxification, and preventing high homocysteine, often taken together in a 1:1 ratio.
Key Aspects of Combining TMG with NAD+ Boosters:
- Why Combine Them: NAD+ boosters help energy production but can drain the body’s methyl group supply. TMG is a "methyl donor," restoring these groups to keep methylation pathways open.
- Optimal Ratio & Dosage: Experts, including Dr. David Sinclair, often recommend a 1:1 ratio of TMG to NMN/NR, commonly around
When to Take: TMG can be taken in the morning, often with food to maximize absorption and prevent stomach discomfort.
of TMG daily. - Synergy: While NMN/NR directly raises NAD+, TMG ensures the metabolic process does not cause long-term depletion of methyl groups, supporting, overall, cellular health.
- Benefits: Potential benefits include sustained energy, improved cardiovascular health, and better methylation, with significant improvements often reported after 3-6 months.
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Fostradamus
GLP-1 Apprentice
I'd like to say yes, but with the caveat that I'm not taking NAD on it's own. My energy levels have increased, but I'm also taking reta, so the increase in energy could be down to weight loss alone, or a combination of both NAD and reta. I was taking Wegovy without NAD prior to starting reta and I have much more energy now than when I was on that. Make of that what you will.
Neptide
GLP-1 Apprentice
Research reveals more energy when using NAD+ with Tirz. NAD+ was a game changer after feeling drained on Tirz.
Foggy-Hollow
GLP-1 Enthusiast
Electrolytes were a game changer for my RS with tirz. Went from napping most of Saturday to functional. (Friday PM shot)
I never increased NAD, I dose 25-50mg three times a week, sometimes less, not on tirz ATM. It didn’t cure the Adhedonia but brought up my energy floor so I’ve kept it.
If you are under 40, your NAD+ recycle and creation systems may not be as depleted. You may not feel as boosted as others if things the case.
Don’t know until you try.
It’s spicy even buffered. I recon 2.5 mL in 250 mg vials then take my time pushing the plunger. Some days it’s not bad at all and others I have to pause 2-3-4 times.
1u= 1mg, keeps the math easy.
Tirz is very robust, my NAD+ notably loses effectiveness after about 4 weeks. I may never buy 500mg vials for this reason.
I never increased NAD, I dose 25-50mg three times a week, sometimes less, not on tirz ATM. It didn’t cure the Adhedonia but brought up my energy floor so I’ve kept it.
If you are under 40, your NAD+ recycle and creation systems may not be as depleted. You may not feel as boosted as others if things the case.
Don’t know until you try.
It’s spicy even buffered. I recon 2.5 mL in 250 mg vials then take my time pushing the plunger. Some days it’s not bad at all and others I have to pause 2-3-4 times.
1u= 1mg, keeps the math easy.
Tirz is very robust, my NAD+ notably loses effectiveness after about 4 weeks. I may never buy 500mg vials for this reason.
How are the effects at that level? I’m about to start the same myself this week
I'm not sure anyone answered your buffering question. A while back NAD+ was reconstituting so acidic that you were basically injecting an acid, which cause serious injection site reactions, if not worse. "Buffering" was supposed to be a more stable and useable PH.
In my experience, even the ones that claimed to be buffered were still acidic. There are big threads on what is the optimum PH. Here is what Gemini AI says:
"While NAD+ is most stable at lower pH levels, the human body's physiological pH is approximately 7.4.
Unfortunately, I have had to test and adjust all of the batches of NAD+ that I have received, even if they claim to be buffered. Buy some PH strips off of Amazon. They are cheap. Buy some injectible Sodium Bicarbonate (for god's sake, not baking soda). And then, it is just trial and error. Different people have different methods. If I'm looking to end up with 5ml in a 500mg vial, I will add 3ml of BAC, and then slowly add sodium bicarbonate and keep testing for PH until it gets in the optimum range. Then, having kept track of how much SB I added, I add the rest of the BAC water and do one final test for PH. I can get it in the range, but the process is not precise. Still, better than injecting acid.
In my experience, even the ones that claimed to be buffered were still acidic. There are big threads on what is the optimum PH. Here is what Gemini AI says:
"While NAD+ is most stable at lower pH levels, the human body's physiological pH is approximately 7.4.
- Irritation Threshold: Injections with a pH below 3.0 or above 9.0 typically cause significant pain, burning, and tissue irritation at the site of administration.
- Injection Site Comfort: A pH of 5.0 to 6.0 is generally tolerable for subcutaneous or intramuscular injections, providing a balance that preserves the potency of the NAD+ for several weeks (when refrigerated) without causing severe discomfort."
Unfortunately, I have had to test and adjust all of the batches of NAD+ that I have received, even if they claim to be buffered. Buy some PH strips off of Amazon. They are cheap. Buy some injectible Sodium Bicarbonate (for god's sake, not baking soda). And then, it is just trial and error. Different people have different methods. If I'm looking to end up with 5ml in a 500mg vial, I will add 3ml of BAC, and then slowly add sodium bicarbonate and keep testing for PH until it gets in the optimum range. Then, having kept track of how much SB I added, I add the rest of the BAC water and do one final test for PH. I can get it in the range, but the process is not precise. Still, better than injecting acid.
Calm Logic
GLP-1 Specialist
I just realized NAD+ has protective skin/collagen benefits, but more on the defensive side compared to proactive GHK-Cu.
For topical use, NAD+ is sold as a serum, as is its precursor NMN. But to be effective, these topical serums require application using something like a dermapen or dermaroller (as discussed in the attached info).
For the dedicated, topical NMN serum is currently in a GB by PWGrove ($85/kit + fees):
For topical use, NAD+ is sold as a serum, as is its precursor NMN. But to be effective, these topical serums require application using something like a dermapen or dermaroller (as discussed in the attached info).
For the dedicated, topical NMN serum is currently in a GB by PWGrove ($85/kit + fees):
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tendency
GLP-1 Enthusiast
Personally I've never had any issues injection wise with any of the buffered NAD+ I've tried from several vendors. 0 ISR from all of them.
Calm Logic
GLP-1 Specialist
But from your old posts, it wasn't much help either subq? So you took by IM instead? Do you prefer oral supplements now?
Just curious since I just started NAD+ today. It stinged somewhat going in. Even though it was a starting dose of 25 mg (and sold as buffered), it gave me an uncomfortable ISR for an hour or so.
I had diluted the 500-mg vial of NAD+ with 3 mL Hospira, but I will add more Hospira (another 3 mL) and am considering IM:
I resorted to IM for gluta to avoid ISRs, and a clinic here seems to do gluta and NAD+ by IM (in addition to IV).
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tendency
GLP-1 Enthusiast
IM does seem to work better for me than subq. Also, the longer I've been on it the more benefits I'm noticing. I take 50mg daily.
Never made any sense, based upon what I know regarding NAD, to take it once or twice per week or dose NAD+ per the mitochondrial rejuvenation protocol you see on here often. The folks that research the NMN/NR precursors are pretty adamant they need to be taken daily and for multiple months to see the full benefits. I don't see why NAD+ injections would be any different.
The NMN/NR orals never did much for me either for whatever reason. I've always diluted my 500mg NAD with 3ml of hospira as well.
tendency
GLP-1 Enthusiast
Actually I was mistaken; I typically recon 500mg of NAD+ with 5ml of hospira, so, maybe that's helping the ISR.
NAD+ dosing seems to be all over the place - not knowing your age or history it's difficult to say so definitely follow a conservative approach titrating up as posted here.
As for myself, I started from 100mg daily and am still there, but I am old and have been experimenting with the precursors for many years before NAD+ injections became a thing. I still have nicotinamide ampoules ready to go as I prefer it over NAD+ sometimes. Just 10mg gives me an instant boost, which sadly doesn't last very long.
In terms of being NAD being buffered.. I've found that even the "buffered" ones sting a lot and I presume this is just label appeal and most of them are unbuffered. I'm too lazy to test the pH and have sodium chloride and sodium bicarb solutions on hand, which I no longer use as I've become used to the sting.
Unfortunately despite being old and low on energy I have not seen much benefit from NAD. I am wondering if I should go up even higher or switch to oral. Maybe I need mots. But for the time being I am pinning away as I have a lot to go through.
As for myself, I started from 100mg daily and am still there, but I am old and have been experimenting with the precursors for many years before NAD+ injections became a thing. I still have nicotinamide ampoules ready to go as I prefer it over NAD+ sometimes. Just 10mg gives me an instant boost, which sadly doesn't last very long.
In terms of being NAD being buffered.. I've found that even the "buffered" ones sting a lot and I presume this is just label appeal and most of them are unbuffered. I'm too lazy to test the pH and have sodium chloride and sodium bicarb solutions on hand, which I no longer use as I've become used to the sting.
Unfortunately despite being old and low on energy I have not seen much benefit from NAD. I am wondering if I should go up even higher or switch to oral. Maybe I need mots. But for the time being I am pinning away as I have a lot to go through.
domin8brix
GLP-1 Apprentice
I tried NAD for like 4ish months and felt ZERO effects. I would feel that weird warm "rush" after the injection, but that was it. No energy, no added weight loss, no nothin! I am wondering if I was not dosing it right, since it seems like it should be going UP vs a front load and then going "down", so I might mix up a fresh vial and try again using protocols I've seen around here.
PAPoots
GLP-1 Enthusiast
I did the NAD+, MOTS-c, and SS-31 protocol that is floating around in various thread. There is probably a copy of it in this thread somewhere. But I got the best benefits from MOTS-c and NAD+ towards the end of the protocol. It felt like taking pre-workout, but without the fast heart rate caffeine causes. Post Mots-c the Nad+ still hit decent on its own for about 2 months, but the effect has been dwindling. So, I might run the protocol again in another month or so. Possibly after running an Epitalon protocol.
PAPoots
GLP-1 Enthusiast
So, I was looking at Amazon Haul last night and there are a ton of various NAD+ oral supplements available. Usually mixed with other longevity supps, like CoQ10, Resveratrol and a few others. Has anyone taken these? And does the NAD+ work anywhere as near as well as taking it SubQ?
Calm Logic
GLP-1 Specialist
Lots of people feel the same way with most of the non-GLP peptides.
The biggest problem with the non-GLPs is they distract from hoarding GLPs, haha.
Tcah2456
GLP-1 Novice
🚫No Source Discussion🚫
Great info I found 15mg motc a week was great on its own so this must be dynamite from reading the potential synergistic effects, could you tell me how you got on with the protocol any sides/ benefits?
Thank you
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