Injectable Buffered NAD500 – Recon, Storage, IM vs SubQ, & Dosage?

[birdwhacker]

It seems like discussion about NAD gets really jumbled between IV from a clinic and IM/subQ, and people talking about oral precursors and confusion about buffered versus unbuffered. Let's cut through all of that:

• Chinese gray NAD, and I would rather die than be supervised by a qualified clinician.

• Buffered injectable NAD, meaning the ph has already been adjusted to be less acidic. Supposedly.

• SubQ and IM injection only, though if you've tried IV and oral too and can make the comparison lets hear it!

I was hoping that you all might be able to clear up some things for me.

I hear that NAD has a short shelf life and loses potency quickly. I assume that this is a myth.

I hear that NAD can gel up easily, sometimes even when using hospira. I assume this mostly applies to un-buffered, but I don't know.

I hear conflicting camps of dosage. Some people say build up blood levels quickly with 50-250mg doses and then do maintainence doses later. Some stay to stay low and slow (25-50) consistently.

Let's hear some experiences if you please 🙂

Edit: Received the NAD from Amino Lair. 3 day shipping to US FLA good prices.

224lb male 6' tall. History of drug abuse so I can usually tell when something is affecting me and I can usually handle higher doses well. I also wasn't supporting my methylation pathways for a long time and would take high dose niacin without precursors.

currently i take methylated b complex, vitamin D calcium potassium magnesium zinc copper TMG and creatine hcl (much better than monohydrate)

Reconned 500mg at room temperature with 3ml hospira. Administered 25mg SubQ. I will probably be doing 50 tomorrow. Noticed some slight tingling followed by a good heady talkative energy and reduced muscle soreness. Went for third run of the day on only 1350 calories. Will update tomorrow.

 

[NBA_MiddleAgedGirl]

It seems like discussion about NAD gets really jumbled between IV from a clinic and IM/subQ, and people talking about oral precursors and confusion about buffered versus unbuffered. Let's cut through all of that:

• Chinese gray NAD, and I would rather die than be supervised by a qualified clinician.

• Buffered injectable NAD, meaning the ph has already been adjusted to be less acidic. Supposedly.

• SubQ and IM injection only, though if you've tried IV and oral too and can make the comparison lets hear it!

I was hoping that you all might be able to clear up some things for me.

I hear that NAD has a short shelf life and loses potency quickly. I assume that this is a myth.

I hear that NAD can gel up easily, sometimes even when using hospira. I assume this mostly applies to un-buffered, but I don't know.

I hear conflicting camps of dosage. Some people say build up blood levels quickly with 50-250mg doses and then do maintainence doses later. Some stay to stay low and slow (25-50) consistently.

Let's hear some experiences if you please 🙂

I'm actually interested in this information as well. I'm new to this world (not Tirz), and I'm interested in adding NAD+. I recall seeing that it has a short shelf life as well. Does that mean the powder has a short life or once reconstituted?

 

[birdwhacker]

I'm actually interested in this information as well. I'm new to this world (not Tirz), and I'm interested in adding NAD+. I recall seeing that it has a short shelf life as well. Does that mean the powder has a short life or once reconstituted?

I'll keep you informed. I'll be starting tomorrow, only on reta otherwise.

What I have heard is that it has a short shelf life once reconned, someone suggested not refrigerating after reconning to help with the gelling issue. I have ten bottles coming so I can play with it a bit

 

[NBA_MiddleAgedGirl]

I'll keep you informed. I'll be starting tomorrow, only on reta otherwise.

What I have heard is that it has a short shelf life once reconned, someone suggested not refrigerating after reconning to help with the gelling issue. I have ten bottles coming so I can play with it a bit

Thank you so much! I hope all goes well. 🙂

 

[nitetrak]

I'm still waiting for my delivery but I will be doing the 14 week mitochondrial protocol starting with NAD+ as soon as it arrives. What I am finding is that the subq people say you don't need to do it IM, and the IM people say it doesn't work as well subq. I tried to research properly and came to the conclusion that IM is for people that want instant results so they can go to the gym. My idea of this may be wrong but that's what is seems to me.

 

[Camlbacker]

I think the NAD schedule on the 14 week mitochondrial program is a good guideline in terms of dosing, starting with a small dose of 25mg to assess tolerance and then titrating up to 50mg and then 100mg over a few weeks. This gives you ample time to get a sense of how NAD jives with your body chemistry. Use plenty of good BAC water, like 5ml for a 500mg vial. NAD is a pretty viscous brew; diluting it makes the whole process easier, from loading the syringe to avoiding injection site reactions. I'm firmly in the IM camp if you're going to 100mg per ml in your dosing. But if you want to try subQ at those smaller doses and see how that works for you, you can have a pretty good idea of your IM vs subQ preference by the time you reach 100mg using that dosing protocol.

 

[Rink123]

I think the NAD schedule on the 14 week mitochondrial program is a good guideline in terms of dosing, starting with a small dose of 25mg to assess tolerance and then titrating up to 50mg and then 100mg over a few weeks. This gives you ample time to get a sense of how NAD jives with your body chemistry. Use plenty of good BAC water, like 5ml for a 500mg vial. NAD is a pretty viscous brew; diluting it makes the whole process easier, from loading the syringe to avoiding injection site reactions. I'm firmly in the IM camp if you're going to 100mg per ml in your dosing. But if you want to try subQ at those smaller doses and see how that works for you, you can have a pretty good idea of your IM vs subQ preference by the time you reach 100mg using that dosing protocol.

Do you take anything else IM? Curious on your perspective/background.

 

[dailychemicals]

I’ve recently gotten interested in NAD+ and have been using it for about three weeks now.

My first 500 mg vial came from a Swiss supplier. I reconstituted it with 3 ml of bacteriostatic water and started with a 50 mg subcutaneous dose. The first thing I noticed was how strong the stinging sensation was 😅. However, I also slept noticeably better afterward, which made me curious to learn more.

After some research, I realized the product was likely unbuffered, which explained the discomfort.

Thanks to a helpful post here on the forum, I learned how to adjust the pH. That made a significant difference.

What worked for me was adding about 2 ml of bacteriostatic water to the vial, followed by roughly 0.7 ml of sodium bicarbonate solution (keeping the pH under around 6). The solution has remained stable in the fridge for the past three weeks.

Currently, I’m taking around 100 mg two to three times per week, and the injections are now much much more comfortable.

On days when I also use GHK-Cu, I sometimes combine both into a single 1 ml syringe to avoid doing two separate injections.

 

[birdwhacker]

I'm actually interested in this information as well.

See my edit. It reconned perfectly with hospira yesterday and spent the night in the fridge.

The injections are fairly painful and sore for a time afterwards. I'm not sure if it needs more sodium bicarb as someone else said above.

Yesterday I did 25mg (15 units) around 5:45pm. Noticed a small boost of energy and went for a run. I had a slightly harder time than usual getting to sleep. Also had itching in the evening and had to double up my daily Allegra/fexofenadine.

This morning, the next day, I went for 50mg followed by another 50mg on the other side. The first 50mg I was able to stay on my feet forx but after the second 50mg I had a little trouble making my protein shake and laid down for an hour. Felt similar to the weakness following a nicotinic acid flush if you've done that.

It's two or three hours later and I guess I'm less drowsy than I normally would be. Hard to quantify an effect, but I do feel there is one.

I'm scared to try IM because of the ph.

Edit: Feels like my glycogen is depleted. Getting head rushes when I stand up and feeling peckish.

 

[Rink123]

Might have to start a conversation if I can’t get a response here, but for anybody that has gotten 1,000mg NAD+ kit/vials how much bac did you use?? I’m saw 5ml for 500mg earlier here, that sounds excessive.

 

[Such1943]

Might have to start a conversation if I can’t get a response here, but for anybody that has gotten 1,000mg NAD+ kit/vials how much bac did you use?? I’m saw 5ml for 500mg earlier here, that sounds excessive.

I have 1k kits, never used them before thou. I was going to start at 5ml BAC and go by feels for the next vial. I ran 500s /w 2.5 ml BAC with no issues.

 

[desinr-gal]

I think the NAD schedule on the 14 week mitochondrial program is a good guideline in terms of dosing, starting with a small dose of 25mg to assess tolerance and then titrating up to 50mg and then 100mg over a few weeks. This gives you ample time to get a sense of how NAD jives with your body chemistry. Use plenty of good BAC water, like 5ml for a 500mg vial. NAD is a pretty viscous brew; diluting it makes the whole process easier, from loading the syringe to avoiding injection site reactions. I'm firmly in the IM camp if you're going to 100mg per ml in your dosing. But if you want to try subQ at those smaller doses and see how that works for you, you can have a pretty good idea of your IM vs subQ preference by the time you reach 100mg using that dosing protocol.

Given the viscosity does it require a bigger needle for pins?

 

[Slogger]

Might have to start a conversation if I can’t get a response here, but for anybody that has gotten 1,000mg NAD+ kit/vials how much bac did you use?? I’m saw 5ml for 500mg earlier here, that sounds excessive.

I use 5 BAC in my 1,000 nad. It cuts the sting - not all the way, I have to take a long time injecting, but I don’t want to add anymore as I’d have to inject way more.

 

[Rink123]

I use 5 BAC in my 1,000 nad. It cuts the sting - not all the way, I have to take a long time injecting, but I don’t want to add anymore as I’d have to inject way more.

Ever used a pen? I imagine I should opt for my savvio as opposed to my auto convi/gansulin here.

 

[NBA_MiddleAgedGirl]

See my edit. It reconned perfectly with hospira yesterday and spent the night in the fridge.

The injections are fairly painful and sore for a time afterwards. I'm not sure if it needs more sodium bicarb as someone else said above.

Yesterday I did 25mg (15 units) around 5:45pm. Noticed a small boost of energy and went for a run. I had a slightly harder time than usual getting to sleep. Also had itching in the evening and had to double up my daily Allegra/fexofenadine.

This morning, the next day, I went for 50mg followed by another 50mg on the other side. The first 50mg I was able to stay on my feet forx but after the second 50mg I had a little trouble making my protein shake and laid down for an hour. Felt similar to the weakness following a nicotinic acid flush if you've done that.

It's two or three hours later and I guess I'm less drowsy than I normally would be. Hard to quantify an effect, but I do feel there is one.

I'm scared to try IM because of the ph.

Edit: Feels like my glycogen is depleted. Getting head rushes when I stand up and feeling peckish.

Thank you for the follow-up! This is extremely helpful. I already deal with fatigue, don't want to end up more tired than I already am.

 

[birdwhacker]

Thank you for the follow-up! This is extremely helpful. I already deal with fatigue, don't want to end up more tired than I already am.

I hear ya! I'm gonna stick with it, so we'll see how my rest is tonight. I've been doing a LOT of cardio and not eating much food at all, so I might have just been low on carbs. Just had a big bowl of cereal that put me at 2000+ for the day.

I'm hoping for either extra energy or better sleep! And honestly I'd be happy to trade better sleep for less energy xD

 

[NBA_MiddleAgedGirl]

I hear ya! I'm gonna stick with it, so we'll see how my rest is tonight. I've been doing a LOT of cardio and not eating much food at all, so I might have just been low on carbs. Just had a big bowl of cereal that put me at 2000+ for the day.

I'm hoping for either extra energy or better sleep! And honestly I'd be happy to trade better sleep for less energy xD

Oh, yeah! HAHAHA lack of calories and lots of cardio will definitely make you sluggish. I'll just follow along and see what happens with your journey. I thought it was promising when I saw your energy buzz from yesterday's review. Good luck. And thank you!

 

[Slogger]

Ever used a pen? I imagine I should opt for my savvio as opposed to my auto convi/gansulin here.

Yes, tried a pen but it was garbage and I never could get the correct dose so I stopped using it. I haven’t made the commitment to buy a more efficient/accurate one yet.

 

[birdwhacker]

Final update:

No issues reconstituting or storing. I haven't been refrigerating it.

Was doing 50mg every other day, now 50mg a day, for a week or so now.

No improvement to baseline energy at all. In-fact, I feel a little bit tired after injecting but I find that if I power through and work out during this window I feel fine.

Today, I did combine it with 3mg of MotsC (first time trying it) and I feel like they synergized pretty well and I was able to get a lot more out of my cardio than usual. Might be worth it to stack with SS31 & MotsC

Sleep quality may have improved marginally. I think I wake up less and sleep deeper.

Overall not worth it, but I'm gonna continue through the kid. Oral NMN/NR with TMG and niacin flushes every now and then seem to be the way to go. I suspect that people who get a lot out of this stuff are overmethylators or haven't supplemented to boost their natural NAD levels.

 

[FartfulCodger]

Now I'm worried about the 1000mg vials I have on the way. Sounds like there's no way I'll be able to work my way through the first vial on a starter dose within a brief shelf life. Guess my wife and pets are the lucky benefactors.

 

[birdwhacker]

Now I'm worried about the 1000mg vials I have on the way. Sounds like there's no way I'll be able to work my way through the first vial on a starter dose within a brief shelf life. Guess my wife and pets are the lucky benefactors.

Lol you'll be fine man. Read the thread. It's probably just an old wives tale.

The better question is how are you going to get the big doses that are called for, because that shit is spicy. Reconstituting 500mg vials with 3ml burns quite a bit with the stuff I got. 150mg would be 90 units.

And the even better question, will it do any good for you 😛

Seems I bought it just as it went out of fashion.

 

[ftv10hb]

Final update:

No issues reconstituting or storing. I haven't been refrigerating it.

Was doing 50mg every other day, now 50mg a day, for a week or so now.

No improvement to baseline energy at all. In-fact, I feel a little bit tired after injecting but I find that if I power through and work out during this window I feel fine.

Today, I did combine it with 3mg of MotsC (first time trying it) and I feel like they synergized pretty well and I was able to get a lot more out of my cardio than usual. Might be worth it to stack with SS31 & MotsC

Sleep quality may have improved marginally. I think I wake up less and sleep deeper.

Overall not worth it, but I'm gonna continue through the kid. Oral NMN/NR with TMG and niacin flushes every now and then seem to be the way to go. I suspect that people who get a lot out of this stuff are overmethylators or haven't supplemented to boost their natural NAD levels.

NAD+ is one of the most sensitive ones out there after reconstitution. It comes in the amber vials because of how sensitive to light it is, and begins to degrade immediately at room temperature. If you haven’t been refrigerating it, it lost its potency a long time ago. I keep mine in the dark in the fridge, 100mg doses 3x/week, definitely notice the clean energy and general feeling of being capable/productive throughout the day. Pin in the AM only, no effect on sleep either way that I can tell.

Other factor with efficacy of NAD+ is age. We basically have a pool of NAD+ in our bodies that begins to leak and diminish with age. Dosing can replenish that pool but not exceed it. 40+ year olds that have a time or lifestyle-diminished pool will see a more noticeable difference when they supplement NAD+, the younger you are from that the higher your NAD+ levels are naturally and so the less noticeable the difference will be when you supplement.

 

[birdwhacker]

NAD+ is one of the most sensitive ones out there after reconstitution. It comes in the amber vials because of how sensitive to light it is, and begins to degrade immediately at room temperature. If you haven’t been refrigerating it, it lost its potency a long time ago.

Where did you learn this? Mine didn't come in amber vials. By what mechanism does it lose its potency? I can feel it pretty distinctly within minutes of the injection, certainly doesn't feel weaker after a week in the cabinet.

Otherwise, I'm glad that it works for you. But I'll stick to my guns and say it doesn't seem worth the trouble given the price and availability of oral precursors.

 

[ftv10hb]

Where did you learn this? Mine didn't come in amber vials. By what mechanism does it lose its potency? I can feel it pretty distinctly within minutes of the injection, certainly doesn't feel weaker after a week in the cabinet.

Otherwise, I'm glad that it works for you. But I'll stick to my guns and say it doesn't seem worth the trouble given the price and availability of oral precursors.

If it didn’t come in amber vials you honestly should have a word with your vendor, i have never heard of it NOT coming in amber vials and the light exposure would have killed it off long before the room temperature did. Results vary for sure and wouldn’t argue with your decision to continue or not one way or the other, but if someone sold you NAD+ in clear vials and didn’t tell you to wrap the vials in foil or something you have legitimate cause to be upset with them because that is definitely standard.

 

[ftv10hb]

Where did you learn this? Mine didn't come in amber vials. By what mechanism does it lose its potency? I can feel it pretty distinctly within minutes of the injection, certainly doesn't feel weaker after a week in the cabinet.

Otherwise, I'm glad that it works for you. But I'll stick to my guns and say it doesn't seem worth the trouble given the price and availability of oral precursors.

If it was largely in the cabinet in constant dark that will have helped, but between the clear vials and the room temperature you definitely lost significant potency. If you’re going to continue anyway my suggestion would be to wrap the vials in foil after reconstitution and keep in the fridge, and see if you notice a difference over the next vial or not, maybe that will help some

 

[birdwhacker]

If you’re going to continue anyway my suggestion would be to wrap the vials in foil after reconstitution and keep in the fridge

I'm gonna be honest, I don't believe a word of this. Nothing against you at all lol, in this business everyone has their ritual they swear by. But thank you regardless, I'll file this away for later and I may try the foil thing just for the hell of it.

Is your NAD spicy? I don't have test strips or anything, it was advertised as buffered, but it burns a fair bit. I understand that the ph shouldn't go above 6, but I don't have a reference for how that should feel under the skin.

Have you ever tried IM?

 

[ftv10hb]

No offense taken, I think if you ask around more or look into it further you will come across the same info tho. Mine is also not spicy at all, and I only do subQ, nothing against IM, just personally was a leap for me to inject myself at all when I started and IM is another step further that I’m just not motivated to take yet lol.

 

[birdwhacker]

No offense taken, I think if you ask around more or look into it further you will come across the same info tho.

Ah, mine is definitely unbuffered then. Figures. Good thing I'm young and my skin can handle it lol.

I have no doubts that I can come across the info, but I do have doubts that it's true in a meaningful sense.

I just blasted 300mg out of curiosity, on top of the 50mg I had earlier in the day, and I can assure you there's been no loss of potency. I can definitely feel it "working," lol, in this case making me feel a tad uneasy.

 

[fremt52958]

I just received my NAD 1000, from a great place. Definitely an amber vial. I have read several dosing recommendations about doing 3ml BAC to the 1000mg and starting at 15units aka 50mg.

Thoughts?

 

[birdwhacker]

I just received my NAD 1000, from a great place. Definitely an amber vial. I have read several dosing recommendations about doing 3ml BAC to the 1000mg and starting at 15units aka 50mg.

Thoughts?

That's what I did, pretty much. 50mg might be a little strong so plan to sit or lay down right after you inject. Make sure you support your methylation pathways afterwards with some tmg/methyl B.