Peptide Myths Busted: Janoshik Founder Sets the Record Straight

[iamiPAC]

Discover how Janoshik founder Peter Magic breaks new ground in peptide testing with formulations that eliminate the need for backwater and maintain their effectiveness for years to come. This interview dives into how lyophilized (freeze‑dried) peptides can stay stable long-term—without extra additives—when stored correctly. Learn why proper storage techniques matter, how to handle your peptides, and what this means for reliability and convenience.

 

[desinr-gal]

Discover how Janoshik founder Peter Magic breaks new ground in peptide testing with formulations that eliminate the need for backwater and maintain their effectiveness for years to come. This interview dives into how lyophilized (freeze‑dried) peptides can stay stable long-term—without extra additives—when stored correctly. Learn why proper storage techniques matter, how to handle your peptides, and what this means for reliability and convenience.

Food for thought! Thank you

 

[byefatlicia]

Discover how Janoshik founder Peter Magic breaks new ground in peptide testing with formulations that eliminate the need for backwater and maintain their effectiveness for years to come. This interview dives into how lyophilized (freeze‑dried) peptides can stay stable long-term—without extra additives—when stored correctly. Learn why proper storage techniques matter, how to handle your peptides, and what this means for reliability and convenience.

Fantastic!!! Thanks for posting

 

[IshimaruKenta]

Man, I got roasted in the STG TG channel for mentioning something he said in this podcast. I only stated that he said it, but they got so up in arms about it. 🤷

 

[byefatlicia]

Man, I got roasted in the STG TG channel for mentioning something he said in this podcast. I only stated that he said it, but rhey got so up in arms about it. 🤷

I'm absolutely sure that there are allot of people who wouldn't agree with him on a few things. Since I am learning from many on here, I want to hear all the feedback.

 

[byefatlicia]

I thought it was Mike from Breaking Bad giving the interview🤣

 

[IshimaruKenta]

I'm absolutely sure that there are allot of people who wouldn't agree with him on a few things. Since I am learning from many on here, I want to hear all the feedback.

I didn't even say that what he said was the only way. I just said that he said it was ok. Yet they ripped into me, deleting my posts, etc. I kinda trust him a bit more than a random person in an internet chat. 🤷‍♂️ But whatever, people will think what they want.

 

[byefatlicia]

I didn't even say that what he said was the only way. I just said that he said it was ok. Yet they ripped into me, deleting my posts, etc.

Kind of defeats the whole "forum" concept. That's not a conversation at that point.
I have respect for him and what he does and his understanding. But I also know I will always use Bac because of the ones who taught me that it's best.

 

[Sun_Seeker]

That's STG for ya.

 

[Peotidethrowaway]

That's STG for ya.

The 17 year olds teaching the 16 year olds how to drive.

 

[BLASIUS]

Very interesting.
I was surprised by the fact that in Europe they don't use bacteriostatic water and instead use physiological water.
Has somebody awareness of that and already used it?

 

[gobytrain]

Man, I got roasted in the STG TG channel for mentioning something he said in this podcast. I only stated that he said it, but they got so up in arms about it. 🤷

Lol, You're misrepresenting what you said in STG for clout here, and you know it.

 

[Morbius]

I ain't listening to the whole thing so I asked an AI to summarize just the technical information. If you listened to the whole thing, did it get it right?

---

Based on the interview transcript, here is a summary of the technical discussions regarding peptide testing and storage:

Peptide Testing

• Janosik, the testing company, tests for three main parameters: purity, identity, and endotoxins.
• The primary method for determining a peptide's identity is mass spectrometry. This technique measures the mass of the peptide and compares it to the expected mass to confirm its composition.
• Purity is tested using high-performance liquid chromatography (HPLC). The purity percentage is determined by dividing the area of the main peptide peak by the total area of all peaks in the chromatogram.
• For endotoxin testing, the company uses the Limulus amebocyte lysate (LAL) method. They test every batch for endotoxins.

Peptide Storage and Handling

• Lyophilized (freeze-dried) peptides should be stored in the freezer at -20°C. Peter Magic states that this is the only correct way to store them for long-term stability.
• When a peptide is reconstituted with a solvent, its shelf life is significantly reduced.
• The interview discusses the difference between using sterile water and bacteriostatic water for reconstitution.
  
  • Sterile water: Once a vial of sterile water is opened, it is no longer sterile and can be a medium for bacterial growth. This means peptides reconstituted with sterile water should be used immediately.
  • Bacteriostatic water: This type of water contains 0.9% benzyl alcohol, which inhibits bacterial growth. This allows the reconstituted peptide solution to remain stable and sterile for approximately 28 days. The presence of benzyl alcohol is not a factor for stability in most peptides.

 

[Airborne Daddy]

Discover how Janoshik founder Peter Magic breaks new ground in peptide testing with formulations that eliminate the need for backwater and maintain their effectiveness for years to come. This interview dives into how lyophilized (freeze‑dried) peptides can stay stable long-term—without extra additives—when stored correctly. Learn why proper storage techniques matter, how to handle your peptides, and what this means for reliability and convenience.

Peter magic sounds like a porn name.... magic peter.

 

[IshimaruKenta]

Lol, You're misrepresenting what you said in STG for clout here, and you know it.

I did not. I just posted what he said. I never advocated for it. I did say that I trusted him more than internet people. That's all. They deleted some of my messaged. What "clout" would I get here? 🤷‍♂️

Later in the day, there was another guy who posted it and got really defensive. I didn't want to start an argument, I was just stating what he said in the video.

 

[IshimaruKenta]

I ain't listening to the whole thing so I asked an AI to summarize just the technical information. If you listened to the whole thing, did it get it right?

---

Based on the interview transcript, here is a summary of the technical discussions regarding peptide testing and storage:

Peptide Testing

• Janosik, the testing company, tests for three main parameters: purity, identity, and endotoxins.
• The primary method for determining a peptide's identity is mass spectrometry. This technique measures the mass of the peptide and compares it to the expected mass to confirm its composition.
• Purity is tested using high-performance liquid chromatography (HPLC). The purity percentage is determined by dividing the area of the main peptide peak by the total area of all peaks in the chromatogram.
• For endotoxin testing, the company uses the Limulus amebocyte lysate (LAL) method. They test every batch for endotoxins.

Peptide Storage and Handling

• Lyophilized (freeze-dried) peptides should be stored in the freezer at -20°C. Peter Magic states that this is the only correct way to store them for long-term stability.
• When a peptide is reconstituted with a solvent, its shelf life is significantly reduced.
• The interview discusses the difference between using sterile water and bacteriostatic water for reconstitution.
  • Sterile water: Once a vial of sterile water is opened, it is no longer sterile and can be a medium for bacterial growth. This means peptides reconstituted with sterile water should be used immediately.
  • Bacteriostatic water: This type of water contains 0.9% benzyl alcohol, which inhibits bacterial growth. This allows the reconstituted peptide solution to remain stable and sterile for approximately 28 days. The presence of benzyl alcohol is not a factor for stability in most peptides.

That AI summary is 100% wrong lol. He said specifically that testing for encotoxins is a waste of money and extremely rare. He also said you could hold the powder in a regular freezer. I don't know what video that AI was referencing, but that's not what he discussed.

Not saying anything about you posting it, just that he didn't talk about that.

 

[Airborne Daddy]

That AI summary is 100% wrong lol. He said specifically that testing for encotoxins is a waste of money and extremely rare. He also said you could hold the powder in a regular freezer. I don't know what video that AI was referencing, but that's not what he discussed.

Not saying anything about you posting it, just that he didn't talk about that.

-20c is a regular freezer isn't it?

 

[MsGizmo]

-20c is a regular freezer isn't it?

-20c = -4f .. so yeah that's real close to the typical freezer.

 

[Trendset_her77]

Discover how Janoshik founder Peter Magic breaks new ground in peptide testing with formulations that eliminate the need for backwater and maintain their effectiveness for years to come. This interview dives into how lyophilized (freeze‑dried) peptides can stay stable long-term—without extra additives—when stored correctly. Learn why proper storage techniques matter, how to handle your peptides, and what this means for reliability and convenience.

Awesome interview! I really enjoyed this. He answered many questions that I was wondering about.

 

[joseblo]

I was intrigued based on the discussion of the LLM summary so I generated one based on the auto-transcript. Seems to do a good job from my 2x listen.

For peptide testing, the most important assays are LC-MS for identification and purity, sterility testing, and endotoxin checks. Heavy metal screening is technically possible, but adds little value as contamination has never been a real-world issue. Failures are relatively uncommon: about five percent of peptides test as incorrect or degraded, sterility failures occur in roughly three to five percent of cases, and endotoxin failures are rarer still.​

​

On storage and handling, properly lyophilized peptides are remarkably stable, lasting years in a refrigerator and over a decade in a freezer. Even after long periods at room temperature, degradation is usually minimal (2-3%). Once reconstituted, peptides are best used within about four weeks under refrigeration; after that point, microbial growth is a greater concern than chemical breakdown. Larger vials should be avoided if they cannot be used within that window. Shaking or injecting bacteriostatic water directly into the vial does not damage the peptide, despite persistent myths. In Europe, simple sterile water or saline is preferred to bacteriostatic water, which is often unreliable from cheap sources and not inherently necessary for peptide preservation, only for sterility.
​

I had it remove the annoying bullet points.

 

[desinr-gal]

I ain't listening to the whole thing so I asked an AI to summarize just the technical information. If you listened to the whole thing, did it get it right?

---

Based on the interview transcript, here is a summary of the technical discussions regarding peptide testing and storage:

Peptide Testing

• Janosik, the testing company, tests for three main parameters: purity, identity, and endotoxins.
• The primary method for determining a peptide's identity is mass spectrometry. This technique measures the mass of the peptide and compares it to the expected mass to confirm its composition.
• Purity is tested using high-performance liquid chromatography (HPLC). The purity percentage is determined by dividing the area of the main peptide peak by the total area of all peaks in the chromatogram.
• For endotoxin testing, the company uses the Limulus amebocyte lysate (LAL) method. They test every batch for endotoxins.

Peptide Storage and Handling

• Lyophilized (freeze-dried) peptides should be stored in the freezer at -20°C. Peter Magic states that this is the only correct way to store them for long-term stability.
• When a peptide is reconstituted with a solvent, its shelf life is significantly reduced.
• The interview discusses the difference between using sterile water and bacteriostatic water for reconstitution.
  • Sterile water: Once a vial of sterile water is opened, it is no longer sterile and can be a medium for bacterial growth. This means peptides reconstituted with sterile water should be used immediately.
  • Bacteriostatic water: This type of water contains 0.9% benzyl alcohol, which inhibits bacterial growth. This allows the reconstituted peptide solution to remain stable and sterile for approximately 28 days. The presence of benzyl alcohol is not a factor for stability in most peptides.

Much of this is wrong.. I thought his statements were very different than above: He actually said you can store lyphollized peptides at room temp for years. In a fridge for a decade and in the freezer for many decades.
He said you dont need to use Bac water, and that in his country they do not; they use sterile water or sterile saline. He said any sterile water from a pharmacy is fine. He even seemed to dislike the Bac water.
Any reconstituted vial is only safe for 4 wks. after that throw it out because things can and do grow inside.
There were many surprises in this interview.