Endotoxins and Sterility?

[Skydweller007]

So I’ve noticed that most peptide vendors test for purity and volume. But most don’t check for endotoxins and sterility? How often are those a problem?

 

[peptideusername]

How often are those a problem?

Basically never.

I have seen a number of endotoxin tests and none of them have ever come close to the USP/FDA limit of 5 EU/mL for parenteral injections. I'm not saying it's impossible, but I am saying it's fairly improbable.
And sterility testing is almost moot when you consider filtering your peptides. Why waste money on a sterility test when you can just spend that money on filters and sterilize your peps yourself?

 

[bbrewer]

Basically never.

I have seen a number of endotoxin tests and none of them have ever come close to the USP/FDA limit of 5 EU/mL for parenteral injections. I'm not saying it's impossible, but I am saying it's fairly improbable.
And sterility testing is almost moot when you consider filtering your peptides. Why waste money on a sterility test when you can just spend that money on filters and sterilize your peps yourself?

So true, I have never seen an endotoxin test of concern. And just because one vial is sterile, does not mean the others are. I point this out in almost every group testing group I have been in. But then we vote and the majority wants to test for both plus heavy metals. Like clockwork someone says "It's only a little extra per vial to be safe and look how much money you are already saving."

 

[frankpeptid]

Basically never.

I have seen a number of endotoxin tests and none of them have ever come close to the USP/FDA limit of 5 EU/mL for parenteral injections. I'm not saying it's impossible, but I am saying it's fairly improbable.
And sterility testing is almost moot when you consider filtering your peptides. Why waste money on a sterility test when you can just spend that money on filters and sterilize your peps yourself?

I don't think syringe filters are able to sterilize everything

 

[peptideusername]

I don't think syringe filters are able to sterilize everything

Feel free to think whatever you want.
But the pore size of the filters I have are smaller than bacteria so it will physically filter bacteria out of the solution. I'm sure you're technically correct, which is the best kind of correct.

My point remains the same: use the money to filter every vial instead of spending that money on testing only one vial.