A few dumb beginner questions on procedures for sema storage and reconstitution

[Morbius]

I have used Rx semaglutide before but I am giving the world of gray a try due to costs. I've done a lot of reading here but I want to confirm a few things. Thanks if you can give me some pointers.

Bacteriostatic water vials
These should be kept in the dark at room temperature, correct?

Dry peptide vials
These should be kept in the freezer, correct?

Reconstituted peptide vials
These should be kept in the refrigerator, correct?

Reconstitution volume
The guidance I see is all over the place, but 1:1 seems like the most common advice.

I have 5 mg vials and I am starting at 0.25 mg dose, titrating up over the months. It seems like 5 mL of water for 5 mg will get me up to 1 mg doses with a 1 cc insulin syringe. But say I am at the top dose of 2.5 mg and using 10 mg vials... Do I reconstitute a 10 mg vial with 10 mL and draw 2.5 mL into a 3 mL syringe? Or do I use 4 mL water so the vial provides 4x 2.5 mg doses of 1 mL each?

I assume there is a maximum and minimum concentration that we want to observe, whatever dose we are taking.

It seems like there is a maximum practical syringe volume we'd want to use, too. I've been assuming that is about 1 mL, the biggest style insulin syringe.

Syringe filters??
I have seen a few references here to the use of syringe filters for added safety, but I have been unable to find a detailed discussion of the topic. I am not even sure it applies to reconstituted peptides like sema. If someone can explain this or point me in the right direction to read more, I would be grateful.

 

[chmuse]

1. That's what I do
2. Yes, preferably in an area that won't have temp fluctuations- I have mine in a ziplock, in a bigger ziplock with ice packets, to keep the temp more stable. If you're up for it, get a deep freeze.
3. Whatever your little heart desires as long as the mg per dose is right. Just make sure it fully dissolves. Rx is usually .5ml/dose regardless of mg of active ingredient, iirc.

Most vials only hold 3-4ml of liquid.

Filtering is personal choice, some do it some don't. It certainly doesn't HURT.

 

[Morbius]

TYVM @chmuse !

You are right, the vials are awfully small. I asked the vendor what the maximum volume was.

I have a deep freezer in the garage so I was going to put the vials in there... inside of a small cooler with ice packs inside, too. That should keep it pretty stable.

 

[chmuse]

TYVM @chmuse !

You are right, the vials are awfully small. I asked the vendor what the maximum volume was.

I have a deep freezer in the garage so I was going to put the vials in there... inside of a small cooler with ice packs inside, too. That should keep it pretty stable.

Don't be tempted by the better price for like, 10-20 mg sema kit if your dose is only .25. it's a better price per dose, but you shouldn't use one vial for that long. Get the 2mg kit (if you can find one) or the 5mg kit.

 

[Morbius]

Yeah I have a 5 mg kit now and it priced out that even if I wasted a little on the way up, it was a better deal than a 2 mg kit.

Edit to add: The vendor said it was a 3 mL vial so no more than 2.5 mL to reconstitute. I'll get smaller syringes so it's easier to draw up.

 

[tul9033]

Yeah I have a 5 mg kit now and it priced out that even if I wasted a little on the way up, it was a better deal than a 2 mg kit.

Edit to add: The vendor said it was a 3 mL vial so no more than 2.5 mL to reconstitute. I'll get smaller syringes so it's easier to draw up.

The vials will hold at least 3ml and I've put almost 4ml to the very top.
The BAC volume is totally up to you. For a 30mg Tirz vial I use 3ml BAC so each 10 units give me 1mg of product. I try to work my recon volume to be as close to my dosing targets.

 

[ftv10hb]

I had some of the same questions, just starting on my own as well, wanted to piggyback off this with a few related questions:

Bacteriostatic water:

It seems the preferred method is at room temperature, I didn't know this ahead of time and have an unused vial in the refrigerator, this is still OK as well tho? And if so for how long?

Dry peptide vials:

I have also seen some say a dark place at room temperature is OK for these, is that correct and if so for how long? I am not looking for storage of more than 1 year currently

Reconstituted peptide vials:

It looks like refrigeration for about one month is the consensus, is that generally correct? What is the longest that could be stretched if necessary and are there signs I should look for to know when to discontinue use and throw it out?

Appreciate any guidance given!

 

[skeptick]

I had some of the same questions, just starting on my own as well, wanted to piggyback off this with a few related questions:

Bacteriostatic water:

It seems the preferred method is at room temperature, I didn't know this ahead of time and have an unused vial in the refrigerator, this is still OK as well tho? And if so for how long?

Dry peptide vials:

I have also seen some say a dark place at room temperature is OK for these, is that correct and if so for how long? I am not looking for storage of more than 1 year currently

Reconstituted peptide vials:

It looks like refrigeration for about one month is the consensus, is that generally correct? What is the longest that could be stretched if necessary and are there signs I should look for to know when to discontinue use and throw it out?

Appreciate any guidance given!

BAC : room temp/dark per Hospira...dry peptides: just about forever in freezer. Practice 1st in 1st out. Reconstituted peptides: General consensus seems to be 30 days, some go much longer with sterile habits being more important that time...