All right, i searched for freezing tirz, specifically the Jano response but...

[Gr33dyOctopus]

Didn't really see it. But know it exists. Apologize in advance if im posting questions that have been answered, just trying to get a handle on all of this.

Ok, my question is, freezing reconstituted tirz. I have this 30ml of bac water and it would be cool to use the whole thing on ten vials. Calm Logic, or Indolent, or someone else thats been helping me along here mentioned freezing bottles of tirz 10 at a time.

That maybe it was Jano that said we can freeze this shit as long as there isnt to many thaw cycles, that even after... a year or two reconstituted tirz is frozen, when thawed there is barely a poentcy loss. Is this true for others? Does anyone have a link to Jano talking about this subject?

As a bonus question has anyone tried KPV? Thoughts on it?

Thanks in advance fuckers!

 

[Sasquatch]

And why not leave the shit in powder form until it's time to pin?

 

[Grogu]

I previously searched and searched for scientific research on freezing reconstituted peptides, and specifically glp-1 medications, and I haven't been able to find anything specific to freezing glp-1s.

I did find one journal article about glp-1 and glucagon levels in frozen human plasma subjected to multiple freeze-thaw cycles. The authors found, to their surprise, little degradation after 3 freeze/thaws. Here is the article:

Stability of glucagon-like peptide 1 and glucagon in human plasma - PMC

To investigate the stability of glucagon-like peptide 1 (GLP-1) and glucagon in plasma under short- and long-term storage conditions. Pooled human plasma (n=20), to which a dipeptidyl peptidase 4 (DPP4) inhibitor and aprotinin were added, was spiked ...

pmc.ncbi.nlm.nih.gov

I think that this is the article that most people refer to when saying it's okay to freeze tirzepatide. But to me, glp-1 and glucagon levels in human plasma is fundamentally different that reconstituted lyophilized peptides in bac water.

I'm really not sure why there would be a reason to freeze reconstituted lyophilized peptides, I would think it would be better to leave those vials in the freezer until you're ready to reconstitute.

 

[Calm Logic]

It seems crazy but the more I looked into it, the more it seemed fine. I don't have any citations/sources though, just secondhand stuff about what Jano said.

People do it without any obvious issues, and something like tirz is not fragile compared to HGH or something like that. HGH is so fragile that it can easily foam if you don't use a vent needle during recon.

One reason for some is convenience:

My current routine is to reconstitute the whole kit at once, then freeze all [big pharma advises not to freeze because of the mechanisms in their autoinjectors], keeping active-use vial in fridge (or even freezer, with quick thaw before each dosing). Then freezing the leftover bac for future use. Your comfort level might vary, and a downside to this is if you get into more elaborate protocols in the future (e.g. a smaller dose, but every 5 days) so carefully label or otherwise keep track of details like actual mg and bac volume in the vials.

I know there are theoretical concerns. But we can die on the the way to the mailbox.

 

[swimmer]

As a bonus question has anyone tried KPV? Thoughts on it?

Thanks in advance fuckers!

I like it as an antiflamitory its good at knocking back the aches and pains. For me its comparable to ibprofin. It is also somewhat effective at relieving migraine headaches.

 

[Calm Logic]

I would also look into ARA 290:

Feature	KPV (Lysine-Proline-Valine)	ARA 290 (Cibinetide)
Mechanism of Action	Inhibits the intracellular NF-κB inflammatory signaling pathway.	Selectively activates the innate repair receptor (IRR) on the cell surface to promote tissue repair and block inflammation.
Efficacy in Trials	Demonstrated efficacy primarily in animal/lab models for inflammation reduction and wound healing.	Proven efficacy in human Phase II clinical trials for reducing neuropathic pain symptoms and promoting nerve fiber regeneration.
Regulatory Status	Investigational (research-grade); no human safety/efficacy data to recommend clinical use.	Investigational drug in human trials (Phase II/III completed/ongoing); not FDA-approved for general use but has Orphan Drug Status for specific conditions.
Administration	Varied in research: topical, oral (in specific formulations), or injections.	Subcutaneous injection (typically 4 mg daily in trials).

Potential Applications in Specific Pain Conditions

Condition	KPV Potential	ARA 290 Potential
Back Pain	Theoretical benefit due to general anti-inflammatory properties, but no specific studies exist.	Potential for neuropathic low back pain if nerve damage is involved; studies are ongoing regarding adjunctive treatments, but direct ARA 290 studies for general back pain are limited.
Knee Pain (Osteoarthritis)	General anti-inflammatory properties might help reduce joint inflammation, but no specific research or clinical data available.	Less specific focus in research; mechanism focuses more on nerve and organ repair than large joint mechanics, but the innate repair receptor activation could have protective benefits for joint tissues.
Sciatica	No specific research. Sciatica involves nerve root inflammation/compression.	High potential. Preclinical studies in rats with sciatic nerve injury showed long-lasting relief from pain symptoms (allodynia) and suppression of spinal cord inflammation.
Carpal Tunnel	No specific research.	High potential. The mechanism promotes nerve regeneration and reduced neuropathic symptoms demonstrated in human trials for small fiber neuropathy, which is directly relevant to nerve compression syndromes like carpal tunnel.

 

[byefatlicia]

But we can die on the the way to the mailbox

I was more likely to die on the way to the mailbox before finding glp1s 📫