Beginner Reconstitution Questions

[ftv10hb]

Searched some for this but couldn't find what I was looking for, any help appreciated!

I am going to be reconstituting myself for the first time, and wanted to make sure I had the proper procedures down before I do anything I can't take back lol.

1. The bacteriostatic water and dry peptides should both be at room temperature to reconstitute? If the water had been in the fridge or the peptides in the freezer how long should they be left out to bring back to room temp?

2. I am working with small 3ml vials, the person who did this for me before just used the small 30ml insulin needles I inject with to reconstitute the vials, is this correct or do I need a different needle size or type for reconstitution?

3. Am I drawing and injecting the water into the peptide in basically the same way that I draw the solution and inject it into the body? By this I mean I draw air into the needle, put it into the water and draw that much water into the needle, and then inject that into the peptide vial and swirl it around to mix, or do I have that wrong?

Thanks in advance for any advice, tips, or corrections

 

[redbullwings]

Hi, you’re on the right track

1.) Temperature: Let both get close to room temperature. Bacteriostatic water: 5–10 mins out of the fridge. Peptide: 20–30 mins if it was frozen. This prevents clumping and helps it dissolve evenly.

2.) Needle choice: Insulin syringes are fine for small vials. Inject slowly to avoid foam or splashing. For very thick peptides, some ppl use a larger needle just for reconstitution, then switch back to the insulin syringe for injection. But you should be okay.

3.) Adding water: You’re correct. Draw the exact amount of Bac water you need. Slowly inject it down the side of the peptide vial, not directly onto the powder.

4.) General tips:

• Always swab the tops of all vials with alcohol wipes before piercing to prevent contamination.
• Keep the vial upright while drawing solution for injection.
• Label vials with date after reconstitution and store in the fridge if not used immediately.

Good luck. The first time can be daunting but hopefully you should be okay 🙂

 

[aquis]

I've never kept my bac water in the fridge. I store it in a drawer so it's always at room temp.

 

[ftv10hb]

Hi, you’re on the right track

1.) Temperature: Let both get close to room temperature. Bacteriostatic water: 5–10 mins out of the fridge. Peptide: 20–30 mins if it was frozen. This prevents clumping and helps it dissolve evenly.

2.) Needle choice: Insulin syringes are fine for small vials. Inject slowly to avoid foam or splashing. For very thick peptides, some ppl use a larger needle just for reconstitution, then switch back to the insulin syringe for injection. But you should be okay.

3.) Adding water: You’re correct. Draw the exact amount of Bac water you need. Slowly inject it down the side of the peptide vial, not directly onto the powder.

4.) General tips:

• Always swab the tops of all vials with alcohol wipes before piercing to prevent contamination.
• Keep the vial upright while drawing solution for injection.
• Label vials with date after reconstitution and store in the fridge if not used immediately.

Good luck. The first time can be daunting but hopefully you should be okay 🙂

Thank you! A dumb question but I have to ask it lol: when you say keep the vials upright while drawing, do you mean like flat on a surface, and I have the needle above it and draw upwards? Or am I holding the vials straight up and down above the needle, and drawing downwards? The second way is the way I have been doing it so far lol

 

[miss-sanne]

Thank you! A dumb question but I have to ask it lol: when you say keep the vials upright while drawing, do you mean like flat on a surface, and I have the needle above it and draw upwards? Or am I holding the vials straight up and down above the needle, and drawing downwards? The second way is the way I have been doing it so far lol

I draw upwards, the vial stands up, I hold it in my hand ,and put the needle above the vial. does it make sense ;-) - I mean you can do both up and down, I just prefer the first.

 

[ftv10hb]

I've never kept my bac water in the fridge. I store it in a drawer so it's always at room temp.

Yea that is my intention going forward but when I was given my first vials of peptides someone reconstituted for me and some additional bac water they were all in the same plastic holder and so I just put them all in the fridge because I didn't know any better lol. So I have one vial of bac water and one vial of unreconstituted peptide in the fridge together right now

 

[redbullwings]

Yea that is my intention going forward but when I was given my first vials of peptides someone reconstituted for me and some additional bac water they were all in the same plastic holder and so I just put them all in the fridge because I didn't know any better lol. So I have one vial of bac water and one vial of unreconstituted peptide in the fridge together right now

It varies, some people store bac water in a drawer and others like me in the fridge. I guess personal preference

 

[ftv10hb]

I draw upwards, the vial stands up, I hold it in my hand ,and put the needle above the vial. does it make sense ;-) - I mean you can do both up and down, I just prefer the first.

That does make sense and sounds easier than my way of doing it lol, especially if it is important to keep the vials straight up and down while drawing

 

[Chili777]

I've never kept my bac water in the fridge. I store it in a drawer so it's always at room temp.

Particularly, Hospira BAC. Pfizer says to store at room temp always. If it's not Hospira, it's kind of an open question once the vial is punctured. People store it both ways.

 

[skeptick]

I draw upwards, the vial stands up, I hold it in my hand ,and put the needle above the vial. does it make sense ;-) - I mean you can do both up and down, I just prefer the first.

What needle are you using to reach down into your vial where at some point you need to reach to bottom? How to suck out last little dregs from vial?

 

[miss-sanne]

What needle are you using to reach down into your vial where at some point you need to reach to bottom? How to suck out last little dregs from vial?

You turn it over, and look inside, you place the tip of the needle just above the membrane.
Otherwise you buy a needle who are long enough to reach the botton. 🙂

 

[miss-sanne]

That does make sense and sounds easier than my way of doing it lol, especially if it is important to keep the vials straight up and down while drawing

it's not important at all, you decide, what's easier for you. You can tilt it a bit, if it's easier for you. 🙂

 

[latviantower]

I just find it easier to invert the vial and barely puncture the septum (rubber membrane), speeds up drawing the liquid. If you’re down to the last bit and want to easily get every drop, I’ll inject a full syringe of air so I’m not fighting a partial vacuum.

I’ve seen lots of resources here, including peptideresearchinstitute.org, that advocate placing both peptide and BAC in the fridge one hour before reconstituting.

I keep my Hospira at room temperature and pop into fridge an hour before. Once punctured, I do keep it in the fridge. Just easier to keep everything in the same place. Really don’t believe it makes any difference.