Do You Filter Your Peptides Before Use?

[AndyPanda]

I didnt know that the easy touch syringes come apart like the luer lock? I thought I needed those. Also Do the blunt syringes pierce the vial stoppers? I assumed not..

The regular EasyTouch diabetic syringes don’t come apart. I buy their syringe barrels and hypodermics separate.

 

[keangkong]

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

I don't filter. I've never heard of any research suggesting that my risk of infection would be significantly reduced by filtering. Some of the benefits that you claim do not, in my admittedly not scientific brain, make much sense. For instance, maximizing the purity of the product seems unlikely: Anything small enough to pass through the filter no matter how impure will get through. The purity numbers that the testing companies provide can be deceiving, however the testing companies will tell you that they only test for things that are close to the target peptide. They don't test for cyanide, for instance. I'm not suggesting any of the Chinese vendors are likely to give you cyanide; that seems more likely something that an American would do.

 

[keangkong]

Hospira recommends against refrigeration because they want you to throw the bottle out after a single use. Refrigeration extends benzyl alcohol potency from about 28 days to 6weeks or more based in published studies. Syringe filtering grey market stuff is utterly critical to maintain safety from stray particles and bacterial contamination. You can get away with stabbing yourself with dirty needles many times, but eventually you’ll get the crud.

Hospira recommends against refrigeration of pure BAC water because the solution is actually more antibacterial at room temperature; the alchohol is less effective at killing and denaturing when it's colder. That's why you don't need to refrigerate. However, peptides are preserved better when refrigerated than at room temperature.

 

[FlowerFairy]

I didnt know that the easy touch syringes come apart like the luer lock? I thought I needed those. Also Do the blunt syringes pierce the vial stoppers? I assumed not..

You don’t need them for vials- you use the 4mm or 13mm syringes to filter from vials. The blunt tips are to filter glass out of the liquid you draw out of glass ampules.

 

[AndyPanda]

Hospira recommends against refrigeration of pure BAC water because the solution is actually more antibacterial at room temperature; the alchohol is less effective at killing and denaturing when it's colder. That's why you don't need to refrigerate. However, peptides are preserved better when refrigerated than at room temperature.

I think @Augu5+1ne ran away a while ago after a bunch of ad hominem attacks on people here didn’t have the results he wanted.

 

[T.Martino_45]

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

This is fantastic information to have. Actually I have been using all sorts of peptide for 2yrs now and had never known to to do this. I am going to order my 0.22 μm PES syringe filters now. Thank you so much for posting!

 

[keangkong]

I think @Augu5+1ne ran away a while ago after a bunch of ad hominem attacks on people here didn’t have the results he wanted.

I personally like the general tone here. People are willing to openly disagree with others but are rarely outright rude.

 

[randompersonrandom]

Something I'm considering while I think about whether or not I want to filter...I'm on 2.5 mg. I have kits of T-30 which, in theory, COULD last eleven weeks apiece, assuming I'm one of the very lucky ones who can just stay on 2.5 mg indefinitely and not need to move up. I'm willing to do an entire demon-summoning-level ritual of sterile technique if that gives me a reasonable chance of making a vial last that long, but I'm undecided on whether that can be made safe enough to actually do. (I haven't done anything yet, just gathering supplies and reading everything while I use up my stockpile of compounded.)

So my question is, do we have any reason to think that filtering might extend the safe-use window of a vial? I'm not immunocompromised and I'm not deeply worried about infection; but I AM keen on being able to get as much out of a vial as I can, and if filtering is likely to help that, then that seems like something well worth learning how to do.

 

[zpped]

Something I'm considering while I think about whether or not I want to filter...I'm on 2.5 mg. I have kits of T-30 which, in theory, COULD last eleven weeks apiece, assuming I'm one of the very lucky ones who can just stay on 2.5 mg indefinitely and not need to move up. I'm willing to do an entire demon-summoning-level ritual of sterile technique if that gives me a reasonable chance of making a vial last that long, but I'm undecided on whether that can be made safe enough to actually do. (I haven't done anything yet, just gathering supplies and reading everything while I use up my stockpile of compounded.)

So my question is, do we have any reason to think that filtering might extend the safe-use window of a vial? I'm not immunocompromised and I'm not deeply worried about infection; but I AM keen on being able to get as much out of a vial as I can, and if filtering is likely to help that, then that seems like something well worth learning how to do.

I typically filter, but wouldn't hesitate to use tirz for that long either way.

 

[randompersonrandom]

I typically filter, but wouldn't hesitate to use tirz for that long either way.

Thank you for answering that. It's been a REALLY complicated time working out what I'm comfortable with, and since I'm already sort of beyond using the regular authoritative sources (like the FDA) just by going this route, there seem to be so many unmade calls to make about what's reasonably safe-ish.

 

[zpped]

Thank you for answering that. It's been a REALLY complicated time working out what I'm comfortable with, and since I'm already sort of beyond using the regular authoritative sources (like the FDA) just by going this route, there seem to be so many unmade calls to make about what's reasonably safe-ish.

to be fair, you also don't really have any idea how my risk tolerance lines up with your risk tolerance. or what information I used to make that decision.

But if you polled most people that have been in this world for a year+ I think you'll find it's a common opinion.

 

[randompersonrandom]

to be fair, you also don't really have any idea how my risk tolerance lines up with your risk tolerance. or what information I used to make that decision.

But if you polled most people that have been in this world for a year+ I think you'll find it's a common opinion.

oh yeah--"Thank you for answering" means "thank you for that data point, it's one more than I had before from someone in this arena." But saying it that second way sounds sarcastic. 😆

 

[pavlovs]

oh yeah--"Thank you for answering" means "thank you for that data point, it's one more than I had before from someone in this arena." But saying it that second way sounds sarcastic. 😆

I'm fairly risk averse, a lot of my posts show that. However, I've gone up to ten weeks on a vial and felt safe. I watched it for cloudiness or other signs of change, and I do filter.

A couple of extra things I do that you don't have to do:

I use the alcohol wipe on the bottle again after I take the injection. Each vial is stored in its own pill bottle that I also clean with an alcohol wipe before putting a fresh vial in. To keep the temperature near constant, the pill bottles are stored in an insulated lunch box in the refrigerator. I mixed up a vial for a friend back in May, he managed to be one of the lucky ones who was able to stay at 2.5 the whole time and used the same vial for several months.

I don't push it past 2 months myself, but I am 54, have diabetes and an autoimmune disease. Also, this stuff is so cheap that erring on the side of caution isn't really very costly.

Good luck!

 

[chmuse]

I'm fairly risk averse, a lot of my posts show that. However, I've gone up to ten weeks on a vial and felt safe. I watched it for cloudiness or other signs of change, and I do filter.

A couple of extra things I do that you don't have to do:

I use the alcohol wipe on the bottle again after I take the injection. Each vial is stored in its own pill bottle that I also clean with an alcohol wipe before putting a fresh vial in. To keep the temperature near constant, the pill bottles are stored in an insulated lunch box in the refrigerator. I mixed up a vial for a friend back in May, he managed to be one of the lucky ones who was able to stay at 2.5 the whole time and used the same vial for several months.

I don't push it past 2 months myself, but I am 54, have diabetes and an autoimmune disease. Also, this stuff is so cheap that erring on the side of caution isn't really very costly.

Good luck!

That's my usual response... We're getting a damn bargain. I'm pretty cavalier with my safety practices- I'll recon on the couch on a old rolling tray I just wiped down with rubbing alcohol, and I'll keep using until it's gone. But if you are worried- I mean, really it's cheap. A t30 goes for around 110-130 now. Even on max dose- 15- that's twenty weeks. Five months. I'd throw some out of I was worried about my immune system without a second thought.

 

[Incognitodoberman]

Hospira recommends against refrigeration of pure BAC water because the solution is actually more antibacterial at room temperature; the alchohol is less effective at killing and denaturing when it's colder. That's why you don't need to refrigerate. However, peptides are preserved better when refrigerated than at room temperature.

I didn't know this and have been storing mine in the fridge with my pen. I appreciate you sharing!

 

[randompersonrandom]

I'm fairly risk averse, a lot of my posts show that. However, I've gone up to ten weeks on a vial and felt safe. I watched it for cloudiness or other signs of change, and I do filter.

A couple of extra things I do that you don't have to do:

I use the alcohol wipe on the bottle again after I take the injection. Each vial is stored in its own pill bottle that I also clean with an alcohol wipe before putting a fresh vial in. To keep the temperature near constant, the pill bottles are stored in an insulated lunch box in the refrigerator. I mixed up a vial for a friend back in May, he managed to be one of the lucky ones who was able to stay at 2.5 the whole time and used the same vial for several months.

I don't push it past 2 months myself, but I am 54, have diabetes and an autoimmune disease. Also, this stuff is so cheap that erring on the side of caution isn't really very costly.

Good luck!

Thank you for that, and @chmuse too, for your thoughts. It's a good point about changing my point of view re: how valuable and precious every last drop is, now that I'm in a place where a vial, when reconstituted, ended up costing like five percent of what it would have if I were getting it the usual way.