Does anybody on here uses ghk-cu with PBS?

[Asm3489]

I have heard that with BAC the ghk cu looses its cooper properties that are crucial for its benefits. Does anybody know if it actually makes a big diffrence and should i consider bying PBS instead of BAC?

 

[PopTart]

I have heard that with BAC the ghk cu looses its cooper properties that are crucial for its benefits. Does anybody know if it actually makes a big diffrence and should i consider bying PBS instead of BAC?

I haven’t heard that. Can you post a link to the source or a quote of what was stated?

Bac water is the recommended diluent for reconstituting GHK-Cu. You can use sterile PBS but it does not contain a preservative so it cannot be used to store the reconstituted pep.

Mixing GHK-Cu with a diluent containing a strong chelator will cause the GHK to uncouple from the Cu. Bac water is not a chelator.

 

[littledragon25]

I've been using ghk-cu with bac water for about 14 months with no issues. I've had great changes to my skin in that time.

 

[ThatNextGril]

I've been using ghk-cu with bac water for about 14 months with no issues. I've had great changes to my skin in that time.

Oh, interesting! What kind of changes have you noticed? I'm currently only pinning tirz, but I've been doing research on which peptides would aid in improving skin. I have eczema and mild rosacea, so skin health/healing is something I've been interested in for quite some time. Just never considered going down the non topical peptide route.

 

[Rolltide61]

Has anyone gotten oily hair from GHK-cu? I'm in my 60's and I went from brittle unruly gray hair to hair that looks much healthier but is oily by nighttime.

 

[littledragon25]

Oh, interesting! What kind of changes have you noticed? I'm currently only pinning tirz, but I've been doing research on which peptides would aid in improving skin. I have eczema and mild rosacea, so skin health/healing is something I've been interested in for quite some time. Just never considered going down the non topical peptide route.

I also have eczema but my dry, patchy skin is a thing of the past. My pores are noticeably smaller, my skin is much more evenly toned, it's smoother, glowier.

 

[ThatNextGril]

I also have eczema but my dry, patchy skin is a thing of the past. My pores are noticeably smaller, my skin is much more evenly toned, it's smoother, glowier.

That's really good to hear. May I ask what dose you started on and what you're at now? I really want to try it, since most anecdotal reports seem to be positive, but I am hesitant to get locked into daily dosing. The weekly tirz is a no-brainer, but the idea of any more than a few jabs each week is preemtively making my injection sites hurt.

 

[littledragon25]

That's really good to hear. May I ask what dose you started on and what you're at now? I really want to try it, since most anecdotal reports seem to be positive, but I am hesitant to get locked into daily dosing. The weekly tirz is a no-brainer, but the idea of any more than a few jabs each week is preemtively making my injection sites hurt.

Oh you'd quake at my routine; I pin 3 times a day minimum, and up to 6 if everything falls on one day.

I started on 1mg which I diluted a lot to try and avoid the sting. Didn't work and hurt like hell. Then I added KPV and it reduced it a bit. Moved up to 2mg, but I split my dose into am/pm. Then I discovered basic and now I get, in total, 2mg cu, 1mg basic, 800mcg KPV per day, split into 2 doses.

 

[Asm3489]

I haven’t heard that. Can you post a link to the source or a quote of what was stated?

Bac water is the recommended diluent for reconstituting GHK-Cu. You can use sterile PBS but it does not contain a preservative so it cannot be used to store the reconstituted pep.

Mixing GHK-Cu with a diluent containing a strong chelator will cause the GHK to uncouple from the Cu. Bac water is not a chelator.

Free metal ions, such as copper(II), are highly reactive and can easily undergo oxidation, reduction, or precipitation depending on their environment. Phosphate-buffered saline (PBS) helps stabilize metal ions primarily by maintaining a consistent, near-neutral pH (around 7.2–7.4), which minimizes copper precipitation as hydroxides or oxides. The phosphate ions in PBS can also weakly interact with copper, helping to keep it dissolved under physiological conditions. In contrast, bacteriostatic water and normal saline lack buffering capacity and typically have slightly acidic to neutral pH values (about 5–7). Without a stable pH, these solutions are more prone to gradual pH shifts that could promote copper oxidation or precipitation, especially over time or when exposed to air.

 

[PopTart]

Free metal ions, such as copper(II), are highly reactive and can easily undergo oxidation, reduction, or precipitation depending on their environment. Phosphate-buffered saline (PBS) helps stabilize metal ions primarily by maintaining a consistent, near-neutral pH (around 7.2–7.4), which minimizes copper precipitation as hydroxides or oxides. The phosphate ions in PBS can also weakly interact with copper, helping to keep it dissolved under physiological conditions. In contrast, bacteriostatic water and normal saline lack buffering capacity and typically have slightly acidic to neutral pH values (about 5–7). Without a stable pH, these solutions are more prone to gradual pH shifts that could promote copper oxidation or precipitation, especially over time or when exposed to air.

I’m sorry but you’re incorrect as it relates to the copper in GHK-Cu. The copper is not a free ion.

 

[Asm3489]

I’m sorry but you’re incorrect as it relates to the copper in GHK-Cu. The copper is not a free ion.

You are right. I didnt know that. Thanks for letting me know!

 

[Anon7482]

Looks like the biggest reasons to use PBS over BAC for GHK-CU are that all the literature about injecting it uses PBS and it doesn't sting like BAC. Downside is you've gotta put everything into new vials.

 

[PablinhoDoFavela]

lol saw that same theory in org

Looks like the biggest reasons to use PBS over BAC for GHK-CU are that all the literature about injecting it uses PBS and it doesn't sting like BAC. Downside is you've gotta put everything into new vials.

50mg vial + 3 ml PBS at 4.5-5 mg injections a day is gonna last 10-12 days, would degradation happen during that timeline?

 

[PopTart]

lol saw that same theory in org

50mg vial + 3 ml PBS at 4.5-5 mg injections a day is gonna last 10-12 days, would degradation happen during that timeline?

Degradation is not the primary issue with PBS but whether or not it is formulated with a preservative and labeled for multi-use dosing. If the OP cannot tolerate benzyl alcohol and intends to use the reconstituted solution over multiple days, then they need to find a product that includes a preservative. At minimum, the PBS needs to be USP sterile (for single-dose injection) as non-sterile is unsafe for injection.

 

[PablinhoDoFavela]

Degradation is not the primary issue with PBS but whether or not it is formulated with a preservative and labeled for multi-use dosing. If the OP cannot tolerate benzyl alcohol and intends to use the reconstituted

solution over multiple days, then they need to find a product that includes a preservative. At minimum, the PBS needs to be USP sterile (for single-dose injection) as non-sterile is unsafe for injection.

I used this one to run a 2 month cycle

https://tribioscience.com/product/life-sciences/common-reagents/1x-dulbeccos-phosphate-buffered-saline-dpbs-without-calcium-and-magnesium-sterile-for-cell-culture/

Each 50mg vial would last me 11 days = 11 injections
Didn’t see any sign of degradation during that time nor I got an infection

 

[Anon7482]

lol saw that same theory in org

50mg vial + 3 ml PBS at 4.5-5 mg injections a day is gonna last 10-12 days, would degradation happen during that timeline?

Same lmao

From what I remember it becomes less effective after five days then loses effect after seven, but that was in BAC water. Maybe PBS keeps it from degrading for longer, but I feel like you shouldn't keep it for more than a week after recon either way.
Personally I'd rather be safe(r) than sorry and recon no more than five days worth at a time, but you could put it all into single use vials if you wanna be safe or just go crazy and keep it till it's cloudy.

I used this one to run a 2 month cycle

https://tribioscience.com/product/life-sciences/common-reagents/1x-dulbeccos-phosphate-buffered-saline-dpbs-without-calcium-and-magnesium-sterile-for-cell-culture/

Each 50mg vial would last me 11 days = 11 injections
Didn’t see any sign of degradation during that time nor I got an infection

Nice, hope it keeps working out. Putting everything into new vials seems like a PITA, but I'd personally like to see more reports before I keep it for that long myself.

Any sting when you inject?

 

[randompersonrandom]

Same lmao

From what I remember it becomes less effective after five days then loses effect after seven, but that was in BAC water. Maybe PBS keeps it from degrading for longer, but I feel like you shouldn't keep it for more than a week after recon either way.
Personally I'd rather be safe(r) than sorry and recon no more than five days worth at a time, but you could put it all into single use vials if you wanna be safe or just go crazy and keep it till it's cloudy.

Nice, hope it keeps working out. Putting everything into new vials seems like a PITA, but I'd personally like to see more reports before I keep it for that long myself.

Any sting when you inject?

It better not degrade that fast; GHK-Cu is a main component of KLOW and my vials of that run around 30 days each.

 

[PablinhoDoFavela]

Same lmao

From what I remember it becomes less effective after five days then loses effect after seven, but that was in BAC water. Maybe PBS keeps it from degrading for longer, but I feel like you shouldn't keep it for more than a week after recon either way.
Personally I'd rather be safe(r) than sorry and recon no more than five days worth at a time, but you could put it all into single use vials if you wanna be safe or just go crazy and keep it till it's cloudy.

Nice, hope it keeps working out. Putting everything into new vials seems like a PITA, but I'd personally like to see more reports before I keep it for that long myself.

Any sting when you inject?

sting, yep, it really depends on technique too, if i pinch n hold my stomach skin a certain way and inject at a straight angel im good. where did u get that info ghk cu degrades after 5 days

idk bout reports, ghk cu's whole reputation is based off a redit thread with pictures of an old lady's timeline progress while on the peptide, not surprised if one day she confesses to have gotten fillers.