Endotoxin levels

[Bigdog0628]

I'm having a hard time understanding Endotoxin levels. What is a safe level or what is the threshold of what I should be reading on a test?

 

[cheesecake]

from a different thread:

Endotoxin Warning

I want to discuss that blaring banner at the top of the forum...but it isn't a discussion thread. How many here regularly test for endotoxins? I admit, I do not. I haven't had an issue (yet) but this does concern me that I am potentially missing a safety signal. For those who have been...

glp1forum.com

Reference: ~5 EU/kg/hour (≈350 EU/hour for a 70 kg person, IV setting)

Key differences:

• That limit assumes slow IV exposure
• A subcutaneous injection is still a bolus relative to that standard, just absorbed more gradually.
• So:
• ~100–150 EU → near or below conservative limits
• ~250–450 EU → approaching or exceeding what’s typically allowed in controlled settings (especially as a bolus-equivalent exposure)

Step 3: What symptoms are likely at these levels?​

~100–150 EU per injection​

• Many people: no obvious symptoms
• Some:
  • Mild fatigue
  • Slight headache
  • Subtle heart rate increase

~200–300 EU per injection​

• Noticeable in a subset of users:
  • Mild “off” feeling within a few hours
  • Occasional chills or temperature sensitivity
  • Heart rate bump (e.g., +10–20 bpm)

~400+ EU per injection​

• More likely to produce a clear pyrogenic response:
  • Chills / mild fever
  • Headache
  • Fatigue
  • Elevated heart rate
  • Possible lightheadedness (mild BP drop)

 

[Grogu]

From Sufa Leeway over at FPPG on TG.

Thoughts on Endotoxin test results

docs.google.com

 

[Bigdog0628]

From Sufa Leeway over at FPPG on TG.

Thoughts on Endotoxin test results

docs.google.com

Thank you this helps a lot

 

[Grogu]

Thank you this helps a lot

Glad you found it helpful. It’s very conservative advice, but solid in my opinion. It provides a framework to consider your own risk tolerance.

 

[Bigdog0628]

Glad you found it helpful. It’s very conservative advice, but solid in my opinion. It provides a framework to consider your own risk tolerance.

Yes I was just under the impression purity and quantity were the biggest factors so thanks

 

[Calm Logic]

Last summer, I thought it would be cool to get T10, so I could recon the 10 mg of tirz with sterile water for immediate injection of basically the whole thing. With my endo and sterility concerns now, that would be a little crazy.

And there can be intra-batch variability with endos, as with sterility and everything else.

NAD+ is the leader of the pack for endos, at least lately. Most NAD+ still tests fine but some are crazy high. (So another reason to avoid large doses of NAD+, to avoid IM use of NAD+, and/or to get endo results for NAD+. Using oral precursors can also lower the amount of NAD+ needed for injection, as can the supplement apigenin.)

 

[Bigdog0628]

Last summer, I thought it would be cool to get T10, so I could recon the 10 mg of tirz with sterile water for immediate injection of basically the whole thing. With my endo and sterility concerns now, that would be a little crazy.

And there can be intra-batch variability with endos, as with sterility and everything else.

NAD+ is the leader of the pack for endos, at least lately. Most NAD+ still tests fine but some are crazy high. (So another reason to avoid large doses of NAD+, to avoid IM use of NAD+, and/or to get endo results for NAD+. Using oral precursors can also lower the amount of NAD+ needed for injection, as can the supplement apigenin.)

So if you are ordering t10 and t20 and t30 would you have to test all them or shouldn’t they all come from same batches?

 

[BNLFL]

So if you are ordering t10 and t20 and t30 would you have to test all them or shouldn’t they all come from same batches?

That'll all be different batches from what I've seen.

 

[HouseCat]

from a different thread:

Endotoxin Warning

I want to discuss that blaring banner at the top of the forum...but it isn't a discussion thread. How many here regularly test for endotoxins? I admit, I do not. I haven't had an issue (yet) but this does concern me that I am potentially missing a safety signal. For those who have been...

glp1forum.com

Reference: ~5 EU/kg/hour (≈350 EU/hour for a 70 kg person, IV setting)

Key differences:

• That limit assumes slow IV exposure
• A subcutaneous injection is still a bolus relative to that standard, just absorbed more gradually.
• So:
• ~100–150 EU → near or below conservative limits
• ~250–450 EU → approaching or exceeding what’s typically allowed in controlled settings (especially as a bolus-equivalent exposure)

Step 3: What symptoms are likely at these levels?​

~100–150 EU per injection​

• Many people: no obvious symptoms
• Some:
  • Mild fatigue
  • Slight headache
  • Subtle heart rate increase

~200–300 EU per injection​

• Noticeable in a subset of users:
  • Mild “off” feeling within a few hours
  • Occasional chills or temperature sensitivity
  • Heart rate bump (e.g., +10–20 bpm)

~400+ EU per injection​

• More likely to produce a clear pyrogenic response:
  • Chills / mild fever
  • Headache
  • Fatigue
  • Elevated heart rate
  • Possible lightheadedness (mild BP drop)

Thanks so much for this info-just what I was looking for!