Filtering and contamination.

[Dos-Dox]

So far I've only tested Mix's T30 orange cap. I have JEEP's as well, but I haven't used it yet. Trying to find non-orange cap (and now black cap) is tough.

J5 has clear cap/ copper crimp (ZJC) and clear cap/red crimp (TCI) T30. He’s also got T15, T20, T40, and higher. You aren’t going to get rock bottom China wholesale prices, but you’ll get decent prices IMO for something that’s well tested and good customer service for domestic product.

Edit to add: it looks like Aavant’s T-30 is pink cap/black crimp.

 

[desinr-gal]

I might have spoken too soon. Started feeling symptoms again (no mucus yet luckily). I think I'm just going to toss the rest of the Mix T30. Despite filtering, it didn't seem to get rid of what I have. I bought 4 kits of Mix T30 and I'm pretty pissed that all the vials I've tried so far, are bad. Now I'm debating whether to recon the T30 from JEEP I have. I'm honestly not excited about any orange cap T30. Has anyone seen a sterility test for the black cap T30?

And since no vendor guarantees sterility, I'm pretty disappointed that I wasted all that money on this.

Since you have a lot of those vials, maybe some group test you could send to would give results definitively

 

[IshimaruKenta]

Sorry to hear you have had a bad time. It is never fun having GI issues. I've had a couple of bad episodes of stomach pain when I was taking Mounjaro so I know how awful it can feel.

I've been using JEEPs orange cap T30 for about a month and have had no problems. It has been circulating since June 25, so I think if there was some issue with it causing widespread problems we would probably have heard of it by now.

I have seen some COAs of T30 orange as high as 36mg (Aurora). If you presume that it's all from the same place then could it be that your injected dose is higher than you think it is? Your symptoms and their onset match what you might see with a dose related side effect rather than something like dysentery.

Shigella - the bacteria that causes dysentery would be extremely unlikely in a a lyophilised vial and it would also need to be consumed orally to infect the gut epithelia where the infection takes. You cannot get dysentery by injecting shigella via the sub cutaneous route.

Peter Magic in his PepTok interview says that the most common failed test is sterility for anything tested at 3-5%, but filtering would almost negate any of that risk. There may be some endotoxin risk, but I'm not sure even that would explain your symptoms. They fit the picture of a dose related side effect much closer. I would not be so quick to throw all your T30 away! Maybe try a lower dose filtered with Hospira and see how that goes. I'd even be tempted to prepare a single dose with sterile water for injections so you have removed any other factors and inject half of what you usually take and see how you feel. I think there is hope for your T30 yet!

That's an interesting wrinkle. I'll admit that I no longer dose based on the COA, since I've been on it for a while. I'm currently on 15mg of Tirz, but even with overfill may be around 17 or 18mg. I'm currently doing 50 units. I'm still on the train of the vials being compromised due to them failing sterility, but I'll try your suggestion of lowering the dose, maybe 35/40 units and see how that goes.

 

[Julep784]

Sorry to hear you have had a bad time. It is never fun having GI issues. I've had a couple of bad episodes of stomach pain when I was taking Mounjaro so I know how awful it can feel.

I've been using JEEPs orange cap T30 for about a month and have had no problems. It has been circulating since June 25, so I think if there was some issue with it causing widespread problems we would probably have heard of it by now.

I have seen some COAs of T30 orange as high as 36mg (Aurora). If you presume that it's all from the same place then could it be that your injected dose is higher than you think it is? Your symptoms and their onset match what you might see with a dose related side effect rather than something like dysentery.

Shigella - the bacteria that causes dysentery would be extremely unlikely in a a lyophilised vial and it would also need to be consumed orally to infect the gut epithelia where the infection takes. You cannot get dysentery by injecting shigella via the sub cutaneous route.

Peter Magic in his PepTok interview says that the most common failed test is sterility for anything tested at 3-5%, but filtering would almost negate any of that risk. There may be some endotoxin risk, but I'm not sure even that would explain your symptoms. They fit the picture of a dose related side effect much closer. I would not be so quick to throw all your T30 away! Maybe try a lower dose filtered with Hospira and see how that goes. I'd even be tempted to prepare a single dose with sterile water for injections so you have removed any other factors and inject half of what you usually take and see how you feel. I think there is hope for your T30 yet!

I ordered T40 from Jeep - orange caps. It’s been not effective at all. I’m wondering if they sent me T30 by accident. Trying to figure why it’s not working so well.

 

[IshimaruKenta]

I'll also add this, this entire time, my nasal mucus has been yellow, which indicates an infection, and I don't have a cold or flu. Not discounting what @GalloB73 said, just adding more evidence that I'm pretty certain it's bacterial.

 

[lessthanhalf]

I am in no way disputing your experiences. I just cannot conceive of a plausible physical mechanism by which a subcutaneous injection containing a bacterial gut pathogen, is going to make it to your gut . There are just way too many tissue boundaries in the way. It would be much easier to believe it could cause a severe systemic infection, that would definitely put you in hospital with septicaemia, than it could get to the gut without that intermediate step. My logic say that it is so implausible that it is not a good explanation, and any other explanation such as food exposure makes more sense.
Filtering is still a good idea.

 

[joseblo]

I am in no way disputing your experiences. I just cannot conceive of a plausible physical mechanism by which a subcutaneous injection containing a bacterial gut pathogen, is going to make it to your gut . There are just way too many tissue boundaries in the way. It would be much easier to believe it could cause a severe systemic infection, that would definitely put you in hospital with septicaemia, than it could get to the gut without that intermediate step. My logic say that it is so implausible that it is not a good explanation, and any other explanation such as food exposure makes more sense.
Filtering is still a good idea.

I got the same worry. The one thing I can think of is systemic immune response. Usually, immune response ISRs are localized, but blood poisoning / sepsis is real, YO!

 

[CNCCurrency]

I got the same worry. The one thing I can think of is systemic immune response. Usually, immune response ISRs are localized, but blood poisoning / sepsis is real, YO!

Plus add a sinus infection. Make that make sense

 

[DjJoshua]

If you don't want to spring for a laminar flow hood, there are also collapsable Still Air Boxes used for Mycology (Mushroom growing) for $30, which can be sprayed down. Best of all, use both!

WOW I just got one off of AMAZON!! they look so slick! (collapsable Still Air Boxes) I think it was $30 bucks. Thanks for the heads up.

 

[joseblo]

WOW I just got one off of AMAZON!! they look so slick! (collapsable Still Air Boxes) I think it was $30 bucks. Thanks for the heads up.

Yeah, it made it possible to move my recon work out to the garage, where I can leave it set up (and covered so the neighbors don't find out I'm Breaking Bad) with spousal consent. Just transport things in and out of the fridge/freezer in a wiped down box or baggy. The alcohol spray and the 3D printed recon stand just await new arrivals.

 

[DjJoshua]

I do have a mix of chlorine i spray down my surfaces before I filter but do you think spraying the inside of this plastic hood would be better with alcohol spray?

 

[mtomm]

I might have spoken too soon. Started feeling symptoms again (no mucus yet luckily). I think I'm just going to toss the rest of the Mix T30. Despite filtering, it didn't seem to get rid of what I have. I bought 4 kits of Mix T30 and I'm pretty pissed that all the vials I've tried so far, are bad. Now I'm debating whether to recon the T30 from JEEP I have. I'm honestly not excited about any orange cap T30. Has anyone seen a sterility test for the black cap T30?

And since no vendor guarantees sterility, I'm pretty disappointed that I wasted all that money on this.

Oh man, I was all for not filtering before...however, after reconning some famous orange cap T30 from a vendor here...starting feeling weird. Gas and bubble guts in the morning, nausea, some fatigue. I just chalked it up to higher doses of both Tirz and Reta. HOWEVER, this morning I noticed something different (apologies in advance...). Got some bad diarrhea, but that turning into something REALLY strange, clear poop with some blood. Looking it up, symptoms showed it wasn't clear, it was actually mucus. Looking up said symptoms, it brought me to bacillary dysentery.

I'll note that multiple T30 vials from different vendors failed sterility. I have so much orange cap T30 and so many vendors carry it. I'll be filtering from now on, as I feel like absolute shit.🤢

I know I'm late to this party. After reading through all the replies and the comment about what Peter Magic had commented about sterility, it kind of confirmed my thoughts about the symptoms of your illness. It sounds an awful lot like runners colitis. Diarrhea with mucus and blood. It very well could be you had a gut reaction or side effect the same way some runners do after long runs. It's stress induced.

Anyway, just a thought and maybe helpful to someone in the future.

 

[IshimaruKenta]

I know I'm late to this party. After reading through all the replies and the comment about what Peter Magic had commented about sterility, it kind of confirmed my thoughts about the symptoms of your illness. It sounds an awful lot like runners colitis. Diarrhea with mucus and blood. It very well could be you had a gut reaction or side effect the same way some runners do after long runs. It's stress induced.

Anyway, just a thought and maybe helpful to someone in the future.

I ended up just reconning a vial of T60 and calculating based on COA. No symptoms after. Could also be that I took too much of a dose than I should, since I was just going off the vial instead of COA amount. But it did get me to start filtering, which is a plus.

 

[CNCCurrency]

I ended up just reconning a vial of T60 and calculating based on COA. No symptoms after. Could also be that I took too much of a dose than I should, since I was just going off the vial instead of COA amount. But it did get me to start filtering, which is a plus.

What no longer celebrating the seahawks?

 

[IshimaruKenta]

What no longer celebrating the seahawks?

Football is done and now it's baseball season. 😄

 

[CNCCurrency]

Football is done and now it's baseball season. 😄

So you skip hockey then? Go Kraken!

 

[OregonSunshine]

So you skip hockey then? Go Kraken!

What is hockey?

(lol, not a huge hockey following here in the PNW).

 

[timj382]

Yeah...you got me. 😂

Personally, can't stop won't stop. Ha.

Here's my question, not knowing what this was, even if the culprit was in the vial(s) you suspect, is there a guarantee filtering would have stopped it? Filtering fails to remove a lot of the more serious issues like endotoxins.

 

[BNLFL]

Where's everyone getting filters and sterile vials? I've looked around a little. I also see a couple sizes of filters, I assume a 13 is the way to go. I'll be using 3ml BAC.

 

[Calm Logic]

I am in no way disputing your experiences. I just cannot conceive of a plausible physical mechanism by which a subcutaneous injection containing a bacterial gut pathogen, is going to make it to your gut . There are just way too many tissue boundaries in the way. It would be much easier to believe it could cause a severe systemic infection, that would definitely put you in hospital with septicaemia, than it could get to the gut without that intermediate step. My logic say that it is so implausible that it is not a good explanation, and any other explanation such as food exposure makes more sense.
Filtering is still a good idea.

What kills me are people who filter but don't use gloves. Staph is everywhere, and washing them only does so much due to oils in the skin, etc. So I use gloves anytime I draw from a vial. I get nitrile gloves when they are on sale at Harbor Freight for like $6 for a box of 100. And they even have XL sizes.

 

[joseblo]

Personally, can't stop won't stop. Ha.

Here's my question, not knowing what this was, even if the culprit was in the vial(s) you suspect, is there a guarantee filtering would have stopped it? Filtering fails to remove a lot of the more serious issues like endotoxins.

Filtering (even 0.22u) will not remove endos or some small viruses, but will get rid of anything that grows. So you only have to worry about what is already in the vial not what can multiply.

For most things endotoxins are not a normal part of the process. NAD and glutathione, and HGH are some of the few (ignoring big Pharma) that are typically made with biologic processes. That means that endotoxins could very well be present, if not removed out by the manufacturer. Most peptides are made by sequentially assembling aminoacids by SPS, which is a chemical process and not particularly likely to have endos (of course you could still have a bacteria or hair in it). Not a bad thing to check, but unlikely except for the ones above or whatever they happen to be making/packaging in the same factory.

I think Uther had a bad batch of NAD lately. Also, the volumes injected tend to be a lot larger.

 

[Calm Logic]

NAD and glutathione, and HGH are some of the few (ignoring big Pharma) that are typically made with biologic processes.

At least HGH is a low dose. Some people will do 400+ mg of glutathione at a time. I limit myself to 200 mg per injection since that is the IM dose at the local clinic. But now that I think about it again, will do no more than 100 mg per injection.

 

[deluge]

Where's everyone getting filters and sterile vials? I've looked around a little. I also see a couple sizes of filters, I assume a 13 is the way to go. I'll be using 3ml BAC.

I was having trouble finding filters for less than $1, then somebody here recommended these at $.51/ea:

Biologix® 13mm Sterile PES Syringe Filter, 0.22µm, 100/pk

High-throughput Biologix® sterile PES syringe filters. Ideal for biological fluid filtration. >98.5% protein recovery. Multiple diameters. Shop now!

biologixusa.com

They sell vials too, but I didn't compare any prices to know if what they have is a good deal.

From the videos I watched 13's are the way to go for volumes >1ml up to 5ml.

 

[J Arbey]

also from the big A, CryoKing 100Pcs Syringe Lab Filter, Sterile PES Hydrophilic Filters, 0.22um

$60/100 count 13mm

 

[Oldnotout]

I'm not a pharmacy, the second I decided to do grey, I accepted, this is not sterile, it never will be.
I do filter though, I do use 10 or more needles per recon, and I practised sterile tecniques in my earlier work life because of operating on patients, so I do feel ok safe.

Good tip, thank you for sharing. Without having an actual lab or knowing why they are designed the way they are, its hard to know practically some of the best practices like that.

 

[joseblo]

I was having trouble finding filters for less than $1, then somebody here recommended these at $.51/ea:

Biologix® 13mm Sterile PES Syringe Filter, 0.22µm, 100/pk

High-throughput Biologix® sterile PES syringe filters. Ideal for biological fluid filtration. >98.5% protein recovery. Multiple diameters. Shop now!

biologixusa.com

They sell vials too, but I didn't compare any prices to know if what they have is a good deal.

From the videos I watched 13's are the way to go for volumes >1ml up to 5ml.

My memory is that Biologix and Cobetter both tested well. Both are available from their websites (much more reliable than AliExp), and Cobetter store used to be available from Amazon as well (though pretty much the same price with free shipping as direct with shipping). There is also Celltreat, which I think has tested ok as well. Any pricing close to $0.50 with shipping is very good.

I have 4mm Tisch, which work fine for me and in volume were under $1 for a 100 box in a 50 box group buy.

Just make sure whatever you get is hydrophilic PES 0.22um and individually wrapped luer lock/slip. Some people prefer 13mm for more concentrated peps (Oils are totally different!), which are cheaper and more available, but you really need to pre-wet and flush, if you're going that way.

 

[deluge]

you really need to pre-wet and flush, if you're going that way.

Now this is interesting, I've seen dozens of people recommend "pre-wetting" the filters, and yet exactly none of the videos I've watched, (also dozens!) does anyone actually pre-wet their filter. The concept seems sound, as does flushing. Does anyone actually do it? Would you mind describing your technique?

 

[fetefille]

Now this is interesting, I've seen dozens of people recommend "pre-wetting" the filters, and yet exactly none of the videos I've watched, (also dozens!) does anyone actually pre-wet their filter. The concept seems sound, as does flushing. Does anyone actually do it? Would you mind describing your technique?

I take a syringe of say .10 of bac, open the filter container (but don't take the label off completely or remove the filter), and slowly push the bac into it. I like to go in a circular motion, but that's an idiosyncrasy I have. Then I go about mixing and once I'm ready to filter, the bac has been absorbed by the filter and I'm good to go, no resistance.

I learned this on my own when I started w/gray a couple of years ago b/c I just couldn't get a good, reliable flow through the filter. Then it hit me--material is too dry, it's actively resisting. Now, some folks don't seem to have any trouble, so idk wtf I was doing wrong, but it was the only solution I could come up with.

 

[deluge]

material is too dry, it's actively resisting. Now, some folks don't seem to have any trouble

Interesting, thank you for sharing.

I did see some reports of people struggling to filter, and it turned out they were trying to filter into the syringe, not while expelling from the syringe. Apparently it's a lot easier to push rather than suck thru a filter.

 

[fetefille]

Interesting, thank you for sharing.

I did see some reports of people struggling to filter, and it turned out they were trying to filter into the syringe, not while expelling from the syringe. Apparently it's a lot easier to push rather than suck thru a filter.

that's odd. I was pushing, not pulling. Never thought about pulling till I saw someone mention it above, seems like you'd waste a filter, and possibly lose more pep than you want.