Filtering. Thread 2.

[Whiynot20026]

So I had a question since I did my first filtering last night. I was very shocked about the pressure I was using to push the reconned fluid through the filter. It litterally felt like I was going to blow the filter apart. I was pushing the plunger hard and the fluid came out nicely and at a good rate. Does this sound right? Or use less pressure and take more time to extract? It seemed like no in between. Either hard pressure or nothing was extracting.

Thanks as aways,
Bill

 

[maokai]

What's the spec of the filter and how much fluid were you filtering

 

[Whiynot20026]

2-3 ml. 0.22um Pore Size, 13mm.

Bill

 

[BNLFL]

Should be an issue. What size needles, and what brand filters?

 

[Whiynot20026]

Should be an issue. What size needles, and what brand filters?

Everything you recommended. Did I put the filters on too tight? I did two of them and they were identical.

Bill

 

[Whiynot20026]

Was I suppose to do anything with the new vial I was putting the fluid into?

 

[Flash-BCR]

They unusually go pretty quick...

 

[Flash-BCR]

Was I suppose to do anything with then vial I was putting the fluid into?

Did you use a vent pin in the sterile vial?...

 

[Whiynot20026]

Did you use a vent pin in the sterile vial?...

So if I ask what a vent pin is, I’m thinking no.

 

[Flash-BCR]

 

[Flash-BCR]

You just get a 25ga syringe needle and stick it in at an angle so the air in the vial has somewhere to go when you are pushing the liquid in...

 

[Whiynot20026]

Shoot. Did not know about that. Thank you. I watched videos on it and no one did that.

 

[Whiynot20026]

You just get a 25ga syringe needle and stick it in at an angle so the air in the vial has somewhere to go when you are pushing the liquid in...

The more you know. Glad I posted. Thanks to all.

Bill

 

[Flash-BCR]

Shoot. Did not know about that. Thank you. I watched videos on it and no one did that.

The more liquid you are putting in the vial (like 3ml in a 3ml vial), the more that air is going to need to get out of there.... 👍

 

[Whiynot20026]

I can feel so stupid sometimes. So basically a brand new sterile vial is the total opposite to a vacuum sealed vial. Now I wish I would have paid more attention in my physics classes.

Bill

 

[BNLFL]

Shoot. Did not know about that. Thank you. I watched videos on it and no one did that.

There you go. Peptide Critic with the rat uses a vent needle.

I can feel so stupid sometimes. So basically a brand new sterile vial is the total opposite to a vacuum sealed vial. Now I wish I would have paid more attention in my physics classes.

Bill

Bingo.

 

[HARLEYV2]

So I had a question since I did my first filtering last night. I was very shocked about the pressure I was using to push the reconned fluid through the filter. It litterally felt like I was going to blow the filter apart. I was pushing the plunger hard and the fluid came out nicely and at a good rate. Does this sound right? Or use less pressure and take more time to extract? It seemed like no in between. Either hard pressure or nothing was extracting.

Thanks as aways,
Bill

Did you have an extra vent needle in the vial you are filling ?

 

[Flash-BCR]

I can feel so stupid sometimes. So basically a brand new sterile vial is the total opposite to a vacuum sealed vial. Now I wish I would have paid more attention in my physics classes.

Bill

You can filter smaller amounts into a vial and not always use vent pin, but your 2ml and 3ml probably created some pretty good back pressure...The pin in the picture looks like it has its own filter and under lab conditions, maybe that would be correct....There has been conversation on air gettin in the sterile vial via the vent pin and compromising sterility, but in my mind, the air is flowing 'out', not in, and I don't worry about it too much...

 

[Whiynot20026]

It’s supper funny when I first started going grey. The thought of reconning my own vials horrified my wife and I. When we bought our 3ML needles for reconning we bought 100 and we both thought we will never use all those. Ya right. When does the madness stop. But that’s what I love about the grey process. The madness never stops.

Bill

 

[Whiynot20026]

You can filter smaller amounts into a vial and not always use vent pin, but your 2ml and 3ml probably created some pretty good back pressure...The pin in the picture looks like it has its own filter and under lab conditions, maybe that would be correct....There has been conversation on air gettin in the sterile vial via the vent pin and compromising sterility, but in my mind, the air is flowing 'out', not in, and I don't worry about it too much...

Could you use a smaller pin like a 30 or 29 and just pull the plunger out?

 

[Whiynot20026]

Did you have an extra vent needle in the vial you are filling ?

No. Just got that tip. Oops.

 

[Flash-BCR]

It’s supper funny when I first started going grey. The thought of reconning my own vials horrified me and my wife. When we bought our 3ML needles for reconning we bout 100 and we both thought we will never use all those. Ya right. When does the madness stop. But that’s what I love about the grey process. The madness does never stop.

Bill

The first times I drew liquid out of a vial, I'd try and hold the syringe and vial, and pull the plunger back and stuff would be flopping...bent more needles than I'd like to admit....😎 I have my "one handed hold technique now'....Like you said, lot to love about it... 👍

 

[Flash-BCR]

The first times I drew liquid out of a vial, I'd try and hold the syringe and vial, and pull the plunger back and stuff would be flopping...bent more needles than I'd like to admit....😎 I have my "one handed hold technique now'....Like you said, lot to love about it... 👍

Get a box of 25ga 5/8" Luer Lock needles (just the needles, no syringe) offa Amazon...pretty cheap for like a 100...

 

[BNLFL]

When does the madness stop. But that’s what I love about the grey process. The madness does never stop.

Could you use a smaller pin like a 30 or 29 and just pull the plunger out?

I bought cheap 23g 1" Luer locks from Amazon for vents.

 

[Whiynot20026]

Awesome. Glad I could get to the bottom of this. Solid information as always and beyond grateful.

Bill

 

[UghItsStupidNIHateIT]

I can feel so stupid sometimes. So basically a brand new sterile vial is the total opposite to a vacuum sealed vial. Now I wish I would have paid more attention in my physics classes.

Bill

See you got this! There’s a learning curve, you’re doing great 😊

 

[Rosco P. Coltrane]

I like you Bill. You're not afraid to ask questions which is good. Lots of good info here that can help out new people. I didnt need any help with filtering but you have asked questions in the past that I was always wondering about.

 

[Whiynot20026]

I like you Bill. You're not afraid to ask questions which is good. Lots of good info here that can help out new people. I didnt need any help with filtering but you have asked questions in the past that I was always wondering about.

I like everyone here so much. Everyone has been wonderful. I’ve learned a ton and I feel what I’ve learned will keep my wife and I safe and healthy. I appreciate that this board is monitored beautifully and everyone here loves to teach.

Bill

 

[Rosco P. Coltrane]

My wife and I are also on this journey together. Im so glad I happen to stumble across this forum last year. Took me about 6 months to make a username lol Lots to search/read on here. Anytime I am bored I hop on here and just read.

 

[BNLFL]

My wife and I are also on this journey together. Im so glad I happen to stumble across this forum last year. Took me about 6 months to make a username lol Lots to search/read on here. Anytime I am bored I hop on here and just read.

Husband and wife deal here as well. She just comes for her shot when I call, and doesn't ask questions. Sometimes I get "that look" if I accidentally make it hurt.