Give me your opinions on my test protocol

[Labcat]

For peptides that are likely stable being frozen as recon, what do you think of reconstituting all the vials in a kit (or 2), pulling an identical specified volume aliquot from each vial and putting those all together into a fresh vial for a COA determining identity and mg (or concentration). Then you would make further vials of the same mix guesstimating 30 days worth (or whatever you’re comfortable with) and freeze those.

I was thinking this would let you know for sure if your kit had the correct peptide in every vial, and you would know the exact concentration of your particular set of randomly filled vials.

The benefit of this would be that all your vials get tested, and you would know for sure what strength you were using.

One protential problem might be that the testing lab gives no significant digits after the decimal. (I saw ProRx released a COA and all it said was 18mg, truly lame.) and of course the other potential problem is finding a high quality lab. As far as I know Janoshik doesn’t accept liquid samples.

 

[Grogu]

I think it would be tough to find a lab to test reconstituted peptides because the reconstitution process brings in all types of variables into the test, such as the quality of the reconstitution agent, degradation, temperature, pH, etc.

But if you were able to find a lab, the method would at least tell you if there is a major issue with the batch or batches, because the results would be the average across all the vials. The test wouldn’t be able to provide any assurance on the strength of individual vials just the average strength and average purity across all vials.

Just say you tested the combined vial from 1 kit of T60 and 9 vials had 70mg and 1 vial had 0mg, the average would be 63mg and the results would look good. Except there is a vial with zero peptides [or some other peptide, which is probably the main concern].

Or if you tests two kits of T60 and one batch has 70mg per vial and the other batch had 0mg per vial. Test would come back with 35mg mass. Then all the vials would be trash because you wouldn’t know which batch has zero mg. Vendor unlikely to reimburse since not a Janoshik test and would argue that the test was with reconstituted peptides and they didn’t do the reconstitution.

 

[yrrdead]

You might see if there are any nearby labs that would lyophilize your sample for you. I also think even without testing this is a neat idea to "standardize" your kit.

I think for someone with the right mindset, training and equipment that this is pretty cool. The other 99.9% of us are going to "send it". I'm looking at you @Airborne Daddy

It is easy for some people to fall into the rabbit hole of needing certainty. You obviously have training and knowledge, so you know that this certainty is an illusion. There is always some % error. Even in the best factories with the best equipment. This paralysis by analysis exists everywhere, fitness, nutrition, business, investing, etc. At some point you need to give up the search for "perfect knowledge" and just NIKE that mother. I know I'm like this, I want to keep researching, save money, be efficient, etc. Though its usually just a way to procrastinate from making an actual decision. At least for me.