How careful are you when reconstituting? air/light/etc

gulangaloid

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mostly referring to the big 3 GLPRAs. while it's common knowledge that light damages them, particularly after reconstitution, is it clear how much? should we be in total darkness with night vision goggles or something while doing it? how deleterious could a minute or two of being in the light while reconstituting be?

also, regarding air, it seems like it's pretty common advice to fill your syringe with air and inject it into the vial, but isn't air supposed to be bad for the peptide? if you keep a vial around for a month, wouldn't at least one or a few injections of air be bad for it?
 
Light won't damage a peptide that fast.. you'll be fine, just put in the fridge, when the solution is clear.
Also, air is fine, but I do vent the vial. Why would you inject air ??
Don't overthink it, just do it.
 
I've never stressed about light exposure, it's only out for a few minutes tops during reconstitution and even less when drawing to pin. I've stopped injecting an equal volume of air in the vial when I draw, only because it makes me feel better. I don't think there's actually any harm in doing it.
 
I've never stressed about light exposure, it's only out for a few minutes tops during reconstitution and even less when drawing to pin. I've stopped injecting an equal volume of air in the vial when I draw, only because it makes me feel better. I don't think there's actually any harm in doing it.
If you inject air or vent you’ll be fine as long as there’s a filter on the needle since these are gone through in 4-6 weeks.
 
I take the vial out of the freezer, reconstitute it on a paper towel on the kitchen counter, and put the vial in a little box in the fridge.

I do inject air into the vial when drawing, I find it close to impossible to draw from the vial without doing gray, the vacuum is too strong.
 
When reconstituting, I'll wipe down the area of the counter I'm working on with alcohol. And when drawing up into the syringe, I'll inject air equal to at least half the amount I plan to draw up, because the vacuum can make it difficult to draw up otherwise. I don't worry about bacteria in the air I'm injecting, but I also wouldn't do it in the bathroom due to the amount of fecal matter usually found in bathroom air. I don't know how much better the air is in the kitchen, or at my desk, but I feel better about it.
 
Thoughts on leaving the vent needle and filter inserted through the stopper during storage? Because I’m lazy and cheap. (I haven’t done it yet but the thought crossed my mind…)
 
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