I’m curious, has anyone ever reconstituted, filtered, then sent out for testing?

[Peevee]

I’m just thinking it might be an interesting experiment to find out if the peps really do all make it through a 22 micron filter.

Of course you’d have to burn two vials to have a fair comparison of before and after

 

[scarywood75]

I’m just thinking it might be an interesting experiment to find out if the peps really do all make it through a 22 micron filter.

Of course you’d have to burn two vials to have a fair comparison of before and after

No need to test that, just look at the molecular size.

A .22 micron filter will start to remove things equal or larger to 220 nanometers.

Peptides like tirzepatide, Semaglutide and retatrutide are small peptide and are about 2 nanometers in size, so there's no way you're gonna filter them out

 

[GimmeABreak]

I’m just thinking it might be an interesting experiment to find out if the peps really do all make it through a 22 micron filter.

Of course you’d have to burn two vials to have a fair comparison of before and after

I think between the margin of error in testing and the variances between vials in the same kit you would be throwing away $600 in testing costs.

The estimate I heard is like .07 mg (or maybe %) in like 2-3ml. I think it was Peptide Test (the guy that sells the filters) that tested it so take that for what it is worth.

I regularly filter compound Tirz when I fill a pen cart with no noticeable difference from dosing directly from the vial. However, feelz is the least accurate measure of anything in our community.

 

[zpped]

No need to test that, just look at the molecular size.

A .22 micron filter will start to remove things equal or larger to 220 nanometers.

Peptides like tirzepatide, Semaglutide and retatrutide are small peptide and are about 2 nanometers in size, so there's no way you're gonna filter them out

While I filter everything and wholly support it, this isn't the complete picture. There is also fluid retention in the filter as well as protein binding to the filter fibers.

 

[GimmeABreak]

While I filter everything and wholly support it, this isn't the complete picture. There is also fluid retention in the filter as well as protein binding to the filter fibers.

True, I pre-wet the filter with BAC and then blow some air through it by leaving a bubble at the top of the syringe with my pre-filtered peps. Not sure how much it helps but again, I don't think we are losing enough to the filter to matter.

 

[scarywood75]

True, I pre-wet the filter with BAC and then blow some air through it by leaving a bubble at the top of the syringe with my pre-filtered peps. Not sure how much it helps but again, I don't think we are losing enough to the filter to matter.

I do the same, I don't feel like much air goes through because of surface tension tho, once it's wet.

 

[scarywood75]

While I filter everything and wholly support it, this isn't the complete picture. There is also fluid retention in the filter as well as protein binding to the filter fibers.

That's fair, you're gonna lose some amount for sure, but not because of the pore size.

To lower protein binding, use PES filters

 

[stickfigure]

I've just ruined a vial of Mod. GRF and a vial of Ipamorelin by filtering. The mix gelled a bit and I guess I did not take time to fully dissolve it through heating or adding more BAC water - started filtering it into the cartridge and it clogged the filter. On the other hand I'd rather have it clog the filter resulting in bunk cartridge than risk injecting polymerized substance.

 

[scarywood75]

I've just ruined a vial of Mod. GRF and a vial of Ipamorelin by filtering. The mix gelled a bit and I guess I did not take time to fully dissolve it through heating or adding more BAC water - started filtering it into the cartridge and it clogged the filter. On the other hand I'd rather have it clog the filter resulting in bunk cartridge than risk injecting polymerized substance.

Yeah, gelled up products can't be filtered much, but at the same time it's not something you'd want to use anyway

 

[teemo2cul]

Can you not reconstitute with 75% of BAC water desired and then push the last 25% after pep is filtered to "clean" the rest of the pep from the filter?

 

[Peevee]

Can you not reconstitute with 75% of BAC water desired and then push the last 25% after pep is filtered to "clean" the rest of the pep from the filter?

I wouldn’t do that because the concentration might be off. There’s some hold back in the filter. So let’s say it’s 10% (it isn’t) but then you’d lose 10% of 75% of your volume instead of 10% of 100% of the volume. I want to filter at the lowest concentration

At 75% a 30 mg vial with a planned volume of 3ML is 13.33 mg per ml. If the homd back is 10% you lose 2.9mg

at 3ML you’d lose 3mg so I guess it doesn’t matter much

 

[zpped]

Can you not reconstitute with 75% of BAC water desired and then push the last 25% after pep is filtered to "clean" the rest of the pep from the filter?

This will help but I find it unnecessary. If you wanted to be super accurate you would also have to add in the fluid retention volume to account for what is left in the filter at the end. And the flushing will still leave some peptide bound to the filter. Its all super overkill though.

I wouldn’t do that because the concentration might be off. There’s some hold back in the filter. So let’s say it’s 10% (it isn’t) but then you’d lose 10% of 75% of your volume instead of 10% of 100% of the volume. I want to filter at the lowest concentration

This isn't the case because the second push would push the higher concentration through and leave the pure bac in the filter.

 

[Peevee]

This will help but I find it unnecessary. If you wanted to be super accurate you would also have to add in the fluid retention volume to account for what is left in the filter at the end. And the flushing will still leave some peptide bound to the filter. Its all super overkill though.


This isn't the case because the second push would push the higher concentration through and leave the pure bac in the filter.

I missed that they were going to bac flush the filter

Either way the volumes are so small that the difference is negligible unless I screwed up the math. My initial thought was that it would be more but I was incorrect

 

[GimmeABreak]

If you pre-wet the filter there is almost no fluid lost to the filter, I also push a little air through it by leaving air at the top of the syringe with the peps.

Needle > Filter > Syringe > Peps > Air

I have reconned with 3ml in a pen and it worked out exactly 3ml of shots.

 

[zpped]

If you pre-wet the filter there is almost no fluid lost to the filter, I also push a little air through it by leaving air at the top of the syringe with the peps.

Needle > Filter > Syringe > Peps > Air

I have reconned with 3ml in a pen and it worked out exactly 3ml of shots.

Agreed, but even pre-wetting isn't really gaining you much. I don't even bother air purging anymore either, but it is the easiest addition. Unless you're using a way oversized filter its just not worth doing all those extra steps.

 

[scarywood75]

Agreed, but even pre-wetting isn't really gaining you much. I don't even bother air purging anymore either, but it is the easiest addition. Unless you're using a way oversized filter its just not worth doing all those extra steps.

For me, I lose a substantial amount of product each time I aliquots, I guesstimate about 0.1-0.3ml total depending of how much liquid I start with.

I am not yet sure where it's all coming from, I suspect there's a bit left in each syringes and needles each time I swap them, even tho I purge them and use low-loss ones.

It seems like I'm losing a bit through evaporation and surface tension, I stopped using evacuated aliquots for this reason and switched to smaller ones, it's better but not perfect.

The filtration is also a place I may be losing some, so I pre-wet and (try to) air purge them too.

All in all, if we add all this, I lose some amount, it sucks I would like to lose nothing since it throws of the dosage and all, I try to take the loss into account, but not a very precise science.

 

[zpped]

For me, I lose a substantial amount of product each time I aliquots, I guesstimate about 0.1-0.3ml total depending of how much liquid I start with.

I am not yet sure where it's all coming from, I suspect there's a bit left in each syringes and needles each time I swap them, even tho I purge them and use low-loss ones.

It seems like I'm losing a bit through evaporation and surface tension, I stopped using evacuated aliquots for this reason and switched to smaller ones, it's better but not perfect.

The filtration is also a place I may be losing some, so I pre-wet and (try to) air purge them too.

All in all, if we add all this, I lose some amount, it sucks I would like to lose nothing since it throws of the dosage and all, I try to take the loss into account, but not a very precise science.

0.3ml is definitely high loss of volume. Are you using something other than cobetter or tisch filters or bigger than 13mm?

 

[scarywood75]

0.3ml is definitely high loss of volume. Are you using something other than cobetter or tisch filters or bigger than 13mm?

Right, I use the double filtration from cobetter 0.45+0.22 or some like that, in 13mn.

I also try to use adapted syringe size to accurately measure, depending of the volume. I now only change the needle after each aliquots, I use the same syringe for 2-5, then switch it too as I figured I must have some left in there too.

I use proper FDA approved vials that cost a fortune lol, properly stored, with the right BAC. Saline BAC is better, less surface tension and liquid seem to "stick" less.

 

[Peevee]

Agreed, but even pre-wetting isn't really gaining you much. I don't even bother air purging anymore either, but it is the easiest addition. Unless you're using a way oversized filter its just not worth doing all those extra steps.

I actually don’t like the 4mm filters as much as the 13mm. Takes too much force to push the fluid through

 

[zpped]

I actually don’t like the 4mm filters as much as the 13mm. Takes too much force to push the fluid through

You should try pushing a 10ml syringe of mct oil through a 23mm filter... Thats a workout.