blueandazure
GLP-1 Novice
I might move up to 7.5 at some point in the near future too, but I just wanted to know if its better to have smaller or larger vials?
I use 5mg every 5 days, should I buy 15mg or 30mg vials?
- Thread starter blueandazure
- Start date
CNCCurrency
GLP-1 Enthusiast
I would buy 60mg, that will last a couple months in the refrigerator
Lyophilized
GLP-1 Apprentice
I buy 120mg, filter \ reconstitute and split in 2 vials (more or less 60mg each). One is freezed until the 1st one ends.
Cheaper this way
Cheaper this way
latviantower
GLP-1 Enthusiast
Let the math guide you. Cheapest $/mg that you can afford. Typically that is the largest sizes available. It’s not going to significantly degrade, even after months in the fridge. Me, I’m happy with 60 mg kits. Good balance.
byefatlicia
GLP-1 Enthusiast
Me personally, I use what I can go through in a month. And what i've noticed is, you usually get T30s for the same price per mg as the T60s 🙂
Its OK to freeze powder.... But explicitly recommended (look at any of the PIs) to NOT freeze reconstituted liquid Tirz.
Or even just do a quick Google search if you don't want to go through the inserts.
blueandazure
GLP-1 Novice
Yeah thanks I think this is the play for me. Cuz I think its true.
I have both T15 and T30. I like the T15 when I am travelling and only need 1 or 2 pins. I like the T30 for home use. Hubby and I share and use up reconned vials in a decent timeframe. We're only pinning 5mg every 5 days. Works for us-
Lyophilized
GLP-1 Apprentice
Sorry to disagree...
If you search a little more you will find info that with Tirzepatide, there is no problem at all.
I work with LCMSMS and already confirmed that the potency is not lost
Try to search and you will find that info
I'm not talking about all the peptides.... I'm just talking about tirze only
In case you want to learn...
indolent
GLP-1 Specialist
I buy 100 or 120 mg kits, recon the whole kit and pop 9 in the freezer. Then I use the active one, refrigerated, for up to 3 months (although at 15mg of tirzepatide, a vial is actually two months for me, max).
This assumes
This assumes
- alcohol-swabbing the top before every puncture (including recon)
- using bac water (but know that it merely slows microbial growth relative to sterile water)
- most of all that each of us has to determine his own risk tolerance.
Lyophilized
GLP-1 Apprentice
You made your homework 👍👍👍 well done
Grogu
GLP-1 Apprentice
Very cool that you work with LCMSMS. Is there any reason to think retatrutide would be any different than tirzepatide as far as freezing since both peptides are made up of 39 amino acids? I only ask because I had planned on doing what you metioned about reconstituting and the freezing a vial but with retatrutide. Is retatrutide that much more complex in structure?
byefatlicia
GLP-1 Enthusiast
So condescending... That means talking down to 🙂
I’m not disputing your LC-MS/MS results or that chemical potency can be detected after freezing. The issue is that manufacturer guidance and package inserts do not recommend freezing reconstituted tirzepatide because stability ≠ safety.
LC-MS/MS can confirm molecular presence, but it does not assess aggregation, degradation pathways, sterility, or changes from freeze–thaw cycles that matter for injectables. That’s why clinical guidance relies on validated stability, not informal testing.
You can choose to do whatever you want personally, I'm all for that if it's your level of comfort..... but presenting freezing as “no problem at all” goes beyond available clinical recommendations and probably is best avoided when there are other options.
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Lyophilized
GLP-1 Apprentice
First
LCMSMS can provide you information about potency, aggregation, degradation because I have access to Orbitrap technology
Second, if you reconstitute correctly as well as filtering with the proper filters and store in proven sterile vials, the sterility will be no problem
Like you I thought that, with Tirzepatide, we should not freeze it after reconstitution
But after reading some studies (ONLY for Tirz) and because I have access to LCMSMS, i checked my own vials that I froze after reconstitute and the results where excellent, with no problems whatsoever. Not all peptides aggregate for your info.. As well as the are some peptides that aggregate right after you reconstitute
I didn't said that after freezing, and use it, I would freeze it again. That can be another story..
Like o said, if you search you'll find some studies
Lyophilized
GLP-1 Apprentice
That was not my intention. Apologies
Hi Grogu
I made this confirmation with my own Tirz because I read more than one study about this and also because I'm taking it
I plan in the future to take Reta, and then I will test also
But I already made some searches about freezing cycles with Reta and didn't find anything credible...
I don't know how Reta performs with at least one freezing cycle (like I did to Tirz)
Having the same amount of aminoacids doesn't mean that they will behave with same physical properties. It's more the type of aminoacids and bonds
When I test in the future I can share to you my results 👍
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Lyophilized
GLP-1 Apprentice
That was not my intention. My apologies
rpm2026
GLP-1 Apprentice
You freeze the reconstituted solution?
Lyophilized
GLP-1 Apprentice
Yes. But just one cycle. After remove the 2nd vial from the freezer I do not freeze again.
Nevertheless there are studies with several cycles and minimal degradation
As well as studies on the opposite, storing reconstituted Tirz at ambient temperature
I leave here one
indolent
GLP-1 Specialist
???
I didn't find it condescending in the least. And I can't figure out how it would be considered so.
[It might or might be condescending for me to correct the usage "made your homework" to "did your homework", though.]
Grogu
GLP-1 Apprentice
Yes, if you would please share the findings of your research about freezing retatrutide in the future that would be fanstatic.
byefatlicia
GLP-1 Enthusiast
No, I'm sorry, that was confusing. I should've added the previous post that when combined came off a bit rough. It's all good🙂
latviantower
GLP-1 Enthusiast
I always assume that in these communities English is not the universal mother tongue. Therefore an extra measure of restraint may be required when word selection seems “rough.”
byefatlicia
GLP-1 Enthusiast
Gr33dyOctopus
GLP-1 Enthusiast
I been freezing tirz and reta, and they are both strong af when i thaw to use. Researched the heck out of this, scientifically bad idea, in real world practice seems to work.fine.
Calm Logic
GLP-1 Specialist
Similarly, many bros on Meso have taken all kinds of foamy/aggregated HGH, and they are fine years later.
Same situation with splitting orfo tablets. Not scientifically established or recommended by Uther, but no issues personally.
Lyophilized
GLP-1 Apprentice
The fact that something was not properly studied doesn't mean that needs to be for sure "scientifically bad idea"
For me "scientifically bad idea" is something that was already studied, it turned as bad, and we keep doing it
Regarding Triz there is already some evidence that this freezing after reconstituted it's not a problem at all
Do you agree?
YoYoFat
GLP-1 Specialist
I have some T15s and T10s that I keep handy for travel, too.
thatkatmat
Recently Joined
This is rather huge news to me...Had I known this and not read over and over and OVER never to freeze Tirz after reconstituting I'd have bought the giant sized Lyophilized and reconstituted, then dibby it up into a ton of small vials which I'm guessing you guys are doing. Thanks for this info!
My research says that freezing a reconstituted vial once is not damaging to the peptide. What is damaging is repeated freeze/thaw cycles that can break the amino bonds. How much this degrades the peptide varies, but it will reduce potency if it's repeatedly done.
Edit: And Tirz is one of the stronger peptides so might be able to withstand it better than most others.
Edit: And Tirz is one of the stronger peptides so might be able to withstand it better than most others.
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