Injectable Buffered NAD500 – Recon, Storage, IM vs SubQ, & Dosage?

[tendency]

...

Other factor with efficacy of NAD+ is age. We basically have a pool of NAD+ in our bodies that begins to leak and diminish with age. Dosing can replenish that pool but not exceed it. 40+ year olds that have a time or lifestyle-diminished pool will see a more noticeable difference when they supplement NAD+, the younger you are from that the higher your NAD+ levels are naturally and so the less noticeable the difference will be when you supplement.

Ehhh, we have a 'pool' of NAD+ that 'leaks' with age? You can then replenish this 'pool'? I'd be very curious to read the studies that settled these assertions scientifically.

 

[tendency]

If it didn’t come in amber vials you honestly should have a word with your vendor, i have never heard of it NOT coming in amber vials and the light exposure would have killed it off long before the room temperature did. Results vary for sure and wouldn’t argue with your decision to continue or not one way or the other, but if someone sold you NAD+ in clear vials and didn’t tell you to wrap the vials in foil or something you have legitimate cause to be upset with them because that is definitely standard.

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

 

[fremt52958]

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

That’s odd my AL was in an amber vial. Just received it yesterday.

 

[birdwhacker]

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

Mine was Amino Lair too.

To be honest, the amber vials light sensitivity thing is clearly nonsense. The product works fine.

I'm more concerned with the fact that the NAD is un-buffered and advertised as buffered. That's directly harmful.

 

[ftv10hb]

Ehhh, we have a 'pool' of NAD+ that 'leaks' with age? You can then replenish this 'pool'? I'd be very curious to read the studies that settled these assertions scientifically.

My poor attempt at describing a visual that makes sense in my head as far as us having NAD stores in our bodies that get depleted over time as natural processes consume more and more of the NAD with out using or replacing it as efficiently as it did when we were younger lol. In my defense tho, “pools” is a term actually used in NAD research haha

Age-related NAD+ decline - PMC

Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite that is reported to decline in concentration in tissues of aged animals. Strategies to increase NAD+ availability have shown promise in treating many conditions in rodents, ...

pmc.ncbi.nlm.nih.gov

 

[ftv10hb]

Mine was Amino Lair too.

To be honest, the amber vials light sensitivity thing is clearly nonsense. The product works fine.

I'm more concerned with the fact that the NAD is un-buffered and advertised as buffered. That's directly harmful.

There were a lot of links here I didn’t want to go through individually, and I certainly don’t claim to understand all the terminology or mechanism at play myself haha, but fwiw imo there’s enough here for me to believe that NAD+ does degrade significantly when exposed to light and/or left at room temperature. Looking at the symptoms of using degraded NAD+ including stinging and temporary fatigue could also explain your experiences with those as opposed to it being unbuffered. But again I am absolutely not any kind of expert, just wanted to at least share the information that convinced me and directs my storage and use of it personally.

 

[ftv10hb]

Yeah, picked up a kit recently from Amino Lair and it was all non-amber vials - first I've seen this happen. Don't think I'll be a repeat customer.

Yea that is strange, I’ve never ordered there but it seems like they’ve been around long enough to know what the standard is or what’s expected. Even stranger that poster below you received in amber vials just recently too, can’t see a reason for same vendor doing it both ways, especially without any explanation or heads up to the customer. Up to you of course but personally I think that justifies refund/replacement if you wanted it.

 

[tendency]

There were a lot of links here I didn’t want to go through individually, and I certainly don’t claim to understand all the terminology or mechanism at play myself haha, but fwiw imo there’s enough here for me to believe that NAD+ does degrade significantly when exposed to light and/or left at room temperature. Looking at the symptoms of using degraded NAD+ including stinging and temporary fatigue could also explain your experiences with those as opposed to it being unbuffered. But again I am absolutely not any kind of expert, just wanted to at least share the information that convinced me and directs my storage and use of it personally.

There was a forum member on here recently that posted an interesting study, darn if I can find it now, however they, or someone they knew? reconned a vial of NAD+ then left it in the fridge. After 15 days they had the NAD+ levels tested by a lab and it came back 50% degraded. So, yes, I agree that once reconned NAD+ has a fairly short shelf life.

I'm not sure, however, how sensitive the lyophilized un-reconned NAD+ powder is. That may very well be less sensitive to degradation. Sure would like to know definitively.

 

[ftv10hb]

There was a forum member on here recently that posted an interesting study, darn if I can find it now, however they, or someone they knew? reconned a vial of NAD+ then left it in the fridge. After 15 days they had the NAD+ levels tested by a lab and it came back 50% degraded. So, yes, I agree that once reconned NAD+ has a fairly short shelf life.

I'm not sure, however, how sensitive the lyophilized un-reconned NAD+ powder is. That may very well be less sensitive to degradation. Sure would like to know definitively.

Yea, I would too, especially considering how much of the powder I have sitting in the fridge right now lol. And if that’s what happens to it reconstituted even in the fridge I should probably switch to the 500mg vials instead of the 1000 lol

 

[Loveandpeaceandjoy]

PGB is one of the top and most recommended suppliers of buffered NAD. They always have clear vials so not sure if folks are really concerned about the amber vials. Have used them exclusively for NAD over the last year and haven’t noticed a difference with effectiveness at 53 yo. 500 mg vial, sub q, refrigerated, typically lasts about a month.

 

[tendency]

PGB is one of the top and most recommended suppliers of buffered NAD. They always have clear vials so not sure if folks are really concerned about the amber vials. Have used them exclusively for NAD over the last year and haven’t noticed a difference with effectiveness at 53 yo. 500 mg vial, sub q, refrigerated, typically lasts about a month.

This is another good data point, thanks.

Curious, since you've been on NAD+ longish term what benefits you've noticed?

 

[birdwhacker]

Looking at the symptoms of using degraded NAD+ including stinging and temporary fatigue could also explain your experiences with those as opposed to it being unbuffered.

I don't mean not to take you seriously, but screenshotting the search page Google AI is not research. By definition of what a language model is, it's going to be repeating old wives tales from Reddit and repeating your own words back to you. Even a well structured Claude prompt will do the same.

The product I received is clearly NAD and is clearly not degraded. The effect it has on me matches practically every description of the effect of NAD, and it feels very similar to niacin and is obviously using methyl groups.

Yea that is strange, I’ve never ordered there but it seems like they’ve been around long enough to know what the standard is or what’s expected.

I went over to his thread to say something about it and the guy is absolutely freaking out. Have a peek at the popcorn: https://glp1forum.com/threads/amino...hipping-signal-aminolair-01.14276/post-157538

There was a forum member on here recently that posted an interesting study, darn if I can find it now, however they, or someone they knew?

I don't buy it. Sounds like a peptide old wives tale to me.

 

[ftv10hb]

I don't mean not to take you seriously, but screenshotting the search page Google AI is not research. By definition of what a language model is, it's going to be repeating old wives tales from Reddit and repeating your own words back to you. Even a well structured Claude prompt will do the same.

The product I received is clearly NAD and is clearly not degraded. The effect it has on me matches practically every description of the effect of NAD, and it feels very similar to niacin and is obviously using methyl groups.


I went over to his thread to say something about it and the guy is absolutely freaking out. Have a peek at the popcorn: https://glp1forum.com/threads/amino...hipping-signal-aminolair-01.14276/post-157538


I don't buy it. Sounds like a peptide old wives tale to me.

lol I agree AI not inherently trustworthy, I prefer to use more as a jumping off point to look into things further. In this case it looked like most of the evidence it cited came from actual NIH studies and pharma sources but I definitely didn’t have the interest level to go through them individually haha. But yea this is the area we’ve chosen to operate in, there’s lots of info out there, lots of it contradictory or unreliable and at the end of the day we all just individually assess it best we can and make our choices lol. That vendor response you linked to completely over the top tho, that’s crazy haha

 

[randompersonrandom]

I went over to his thread to say something about it and the guy is absolutely freaking out. Have a peek at the popcorn: https://glp1forum.com/threads/amino...hipping-signal-aminolair-01.14276/post-157538

I'm so unhappy watching that. That guy's prices are high, but I've bought from him before and was glad to have an option to buy from him again if I was ever in a pinch, but "has never had a big-feelings fight with a customer in a public space" is one of my prerequisitives for "will buy from this vendor." I also didn't love how huffy he got over people wanting him to post a price list. No I'm not going to install signal to see whether your epi has come down, thanks anyway.

 

[birdwhacker]

... was glad to have an option to buy from him again if I was ever in a pinch, but "has never had a big-feelings fight with a customer in a public space" is one of my prerequisitives for "will buy from this vendor."

Yeah, pretty obvious that no matter what the issue is he will never take any responsibility whatsoever. It's a shame because I think we'd all love to have someone here in the US we could fully get behind, who runs a tight ship and takes those kind of payment options.

At least a Chinese vendor will have the good sense to treat you with respect while they screw you over.

 

[Loveandpeaceandjoy]

This is another good data point, thanks.

Curious, since you've been on NAD+ longish term what benefits you've noticed?

Def more energy. The Triz had me fatigued badly. Also focus because Meno brain is real😂. I’ve also noticed more weight loss than when cycled off of NAD. I read somewhere it helps with metabolism. And my skin is great but also cycle KLOW so it’s probably a combo of the two.

 

[birdwhacker]

I read somewhere it helps with metabolism.

I could definitely see this happening! Way hungrier on NAD days.

I wish I got the energy from it. Maybe I'm too young or maybe it's because I used to use flush niacin a lot. SS31 and MotsC on the way though! MotsC definitely helps my cardio performance.

 

[Hotlongs]

Yeah, pretty obvious that no matter what the issue is he will never take any responsibility whatsoever. It's a shame because I think we'd all love to have someone here in the US we could fully get behind, who runs a tight ship and takes those kind of payment options.

At least a Chinese vendor will have the good sense to treat you with respect while they screw you over.

I honestly think Amino Lair is a middle aged mentally sus man. I will say that every interaction with him felt unnecessarily hostile.

I don't think any vendor should be trusted if they yell at you for discussing an issue with an order that they refuse to address with you privately.

 

[birdwhacker]

One more bump for the world's dumbest NAD thread...

SO, I got my grubby paws on some Sodium Bicarbonate For Injection (SBFI) that my mom yoinked from her place of work. Thanks mom!

I decided to combine a little with my Un-Buffered NAD That Was Advertised As Buffered (UBNTWAAB).

Note that I don't have any pH testing strips on hand.

So first I tried adding 20 units of the 8.4% SB solution to one of my reconned, half used vials of NAD500 (with 3ml water). It bubbled and sizzled. Good sign. Injected about 50mg and the sting was still about the same. So I added another 60 units of SB, then tried another 50mg. Sting was still there but not bad at all, right about where I want it.

Next experiment was reconning a new vial. I tried 1.8ml of BAC water and 1.2ml of sodium bicarbonate. I think I overshot the mark a bit. Almost no sting. Also, I made the mistake of adding the SB before the NAD has fully reconned in the BAC water. After injecting a few hundred milligrams of this new solution, I suspect that there has been a slight loss in potency. Maybe 25%, based on what I normally feel when injecting NAD.

Obviously I need test strips, but I think the next vial should be perfect with 2ml BAC and 1ml SB, maybe a little less SB if I need extra shelf life.

NAD+ is one of the most sensitive ones out there after reconstitution. It comes in the amber vials because of how sensitive to light it is

So it turns out, NAD has this reputation once being buffered with sodium bicarbonate. Unbuffered NAD has a shelf life of months.

The amber vials thing, I just don't understand. J5 is a highly reputable vendor and he's currently selling buffered NAD in clear vials as well.

 

[tendency]

One more bump for the world's dumbest NAD thread...

SO, I got my grubby paws on some Sodium Bicarbonate For Injection (SBFI) that my mom yoinked from her place of work. Thanks mom!

I decided to combine a little with my Un-Buffered NAD That Was Advertised As Buffered (UBNTWAAB).

Note that I don't have any pH testing strips on hand.

So first I tried adding 20 units of the 8.4% SB solution to one of my reconned, half used vials of NAD500 (with 3ml water). It bubbled and sizzled. Good sign. Injected about 50mg and the sting was still about the same. So I added another 60 units of SB, then tried another 50mg. Sting was still there but not bad at all, right about where I want it.

Next experiment was reconning a new vial. I tried 1.8ml of BAC water and 1.2ml of sodium bicarbonate. I think I overshot the mark a bit. Almost no sting. Also, I made the mistake of adding the SB before the NAD has fully reconned in the BAC water. After injecting a few hundred milligrams of this new solution, I suspect that there has been a slight loss in potency. Maybe 25%, based on what I normally feel when injecting NAD.

Obviously I need test strips, but I think the next vial should be perfect with 2ml BAC and 1ml SB, maybe a little less SB if I need extra shelf life.



So it turns out, NAD has this reputation once being buffered with sodium bicarbonate. Unbuffered NAD has a shelf life of months.

The amber vials thing, I just don't understand. J5 is a highly reputable vendor and he's currently selling buffered NAD in clear vials as well.

Interesting experiment.
I think we bought some of our NAD+ from the same vendor - initials AL - and mine stings like bejesus when I inject it as well. Something definitely off with the PH and sure seems like it's probably unbuffered to me as well.

 

[birdwhacker]

Interesting experiment.
I think we bought some of our NAD+ from the same vendor - initials AL - and mine stings like bejesus when I inject it as well. Something definitely off with the PH and sure seems like it's probably unbuffered to me as well.

Oh did I not call out Amino Lair in my post? I meant to. They freaked out on me when I politely asked them to make sure their product is labeled correctly.

Yeah, we have the same stuff and it's super acidic. You need to get yourself some sodium bicarbonate 8.4% and add that when reconning.

 

[ZippityDooDah]

You all have made your dissatisfaction with Amino Lair clear in many, many posts, and that's fine.

The endless piling-on is not.

If you can't discuss the various aspects of NAD or anything else without constantly bringing up your vendor grudges then we can lock this thread along with any others featuring the same endless griping.

 

[birdwhacker]

You all have made your dissatisfaction clear in many, many posts, and that's fine.

And you know what, while I'm at it, I'm not satisfied with American military action in Iran either!

Problems everywhere you look, I swear.

 

[redhead]

I’ve been researching NAD+ so heavy of late while pinning it. Im late 50s meno woman. Working in a high stress job and with high demand right now. Menopause and Tirz made me tired and brain fogged. NAD def helps with the energy. I notice better stamina throughout the day. No 3pm slump.

I started at 25 daily and now alternate to
50 mg daily. 50 Gets the tingles and rush going. But I do notice a consistent energy and focus throughout the day since I started about a month ago.

I put mine in pens and keep in the fridge.

I finished one bottle reconned. Didn’t notice any degrading in the efficacy. Still got
The rushing feeling.

Love NAD+ and def will keep using it in cycles.

Miss it this week I ran out and need to make a new pen. Couldn’t figure out why today I was so tired by 3pm. Maybe it’s bc I’ve missed NAD for 3 days. ?!?