is it normal to lose ~3/4ml when filtering?

[tendency]

Just started filtering my peptides and was surprised to see I lose about 3/4 of a ml when doing so due to 'slop' in the filter and needle. Is this normal? I can't imagine people are losing this much each time they filter?

 

[miss-sanne]

yes the solution is sitting in the filter, flush it out with maybe 0,5-1 ml of bac.

 

[tendency]

yes the solution is sitting in the filter, flush it out with maybe 0,5-1 ml of bac.

Ah that's a good idea - I knew there was a nice simple one somewhere in there that I'm too boneheaded to figure out 😆 - thanks!

 

[Nathanologist]

This is why we love quantified overfills. End up with the net you paid for even after losses.

 

[50ShadesGreyMarket]

Just started filtering my peptides and was surprised to see I lose about 3/4 of a ml when doing so due to 'slop' in the filter and needle. Is this normal? I can't imagine people are losing this much each time they filter?

I mentioned this in my post about filtering but was told it’s not a real thing despite the fact you can literally SEE the peptide in the filter (GHK-Cu is blue). I prime before and flush afterward with bac but there’s no way to tell the exact amount in the filter. Somehow ‘random person’ figured out how to prevent the filter from absorbing, I assume she’s not using regular filters like we are, I’d ask her how she manages.

 

[nonyabizznez]

3/4 ml? 75 units? Sounds like a big filter. The following summarizes what I've been able to find on the subject:

You want to use the smallest possible filter as a lot of peptide gets left in the filter when you are done using it. For a few approximate examples, a 4mm filter retains about.07ml of reconstituted peptide, a 13mm retains 4x more than a 4mm at about.28ml and a 33mm filter holds a whopping.65ml.

Realistically for individuals filter reconstituted peptides you should use a 4mm filter if you are filtering less than 4ml of peptide or a 13mm filter if you are using more than that. (Source - Internet Floatilla)

I dilute so injections are not so concentrated that a few units either way doesn't make a difference. Ideally .50ml (50 units) and I try to not go lower than .30ml (30 units). I also pre wet the filter by having a bit extra bac in the barrel after reconning lyophilized vials. I remove the recon needle, pop on the filter and plunge the remainder out.

 

[50ShadesGreyMarket]

3/4 ml? 75 units? Sounds like a big filter. The following summarizes what I've been able to find on the subject:

You want to use the smallest possible filter as a lot of peptide gets left in the filter when you are done using it. For a few approximate examples, a 4mm filter retains about.07ml of reconstituted peptide, a 13mm retains 4x more than a 4mm at about.28ml and a 33mm filter holds a whopping.65ml.

Realistically for individuals filter reconstituted peptides you should use a 4mm filter if you are filtering less than 4ml of peptide or a 13mm filter if you are using more than that. (Source - Internet Floatilla)

I dilute so injections are not so concentrated that a few units either way doesn't make a difference. Ideally .50ml (50 units) and I try to not go lower than .30ml (30 units). I also pre wet the filter by having a bit extra bac in the barrel after reconning lyophilized vials. I remove the recon needle, pop on the filter and plunge the remainder out.

That’s exactly what I do for the same reasons. Great minds! 🥹