MIX sterility fail

[fatty33]

Saw this posted at STG about a MIX sterility fail

 

[Jfrick11]

Sterility fails are very very common. That's why most people don't test for them, why there are so many conversations about using Bac water and so many conversations about filtering.

 

[geoguy78]

Sterility fails are very very common. That's why most people don't test for them, why there are so many conversations about using Bac water and so many conversations about filtering.

This is kind of what I was getting at in my comment on the other thread about people testing obsessively. Sterility testing is a prime example. We have immune systems, we cut and puncture ourselves with objects less sterile than these peps all the time. If you're worried about it, just buy a cheap filter and use good quality bacteriostatic water rather than spend hundreds on a test.

 

[IshimaruKenta]

Some princesses make such a big deal out of this. When ABC had a failed test, a few "ladies" told me to kill myself because I told them this wasn't a big deal.

 

[yrrdead]

Some princesses make such a big deal out of this. When ABC had a failed test, a few "ladies" told me to kill myself because I told them this wasn't a big deal.

Is Jano running a qualitative or quantitative ? I'm assuming the former since he just puts fail and I would assume it is faster/cheaper to run.

 

[Chucky]

Some princesses make such a big deal out of this. When ABC had a failed test, a few "ladies" told me to kill myself because I told them this wasn't a big deal.

Wow, that's a tad extreme.

 

[Chili777]

I had questions about what was left in the remainder of the ~1% that isn't peptide on test results. Got this from Gemini and thought it was interesting:

Process-Related Impurities

These are non-peptide materials that remain from the reagents and solvents used during the synthesis and purification steps.

• Residual Solvents and Reagents: The final step of peptide synthesis often involves cleavage from the solid support using strong acids like Trifluoroacetic Acid (TFA). Some of this acid remains, and the peptide is often isolated as a TFA salt. Other solvents or scavengers (reagents used to prevent unwanted side reactions) can also remain.

• Salts and Buffers: The purification step, typically High-Performance Liquid Chromatography (HPLC), uses various buffers and salts. Trace amounts of these can remain in the final lyophilized (freeze-dried) powder.

• Elemental Impurities: Trace amounts of heavy metals or other elements that may have been present in the raw materials or from the reaction vessel can be present.

• Contaminating Peptides: In some rare cases, if multiple peptides are synthesized in the same facility, cross-contamination with a small amount of a completely different peptide can occur.

 

[tncc_rn]

I had questions about what was left in the remainder of the ~1% that isn't peptide on test results. Got this from Gemini and thought it was interesting:

Process-Related Impurities

These are non-peptide materials that remain from the reagents and solvents used during the synthesis and purification steps.

• Residual Solvents and Reagents: The final step of peptide synthesis often involves cleavage from the solid support using strong acids like Trifluoroacetic Acid (TFA). Some of this acid remains, and the peptide is often isolated as a TFA salt. Other solvents or scavengers (reagents used to prevent unwanted side reactions) can also remain.

• Salts and Buffers: The purification step, typically High-Performance Liquid Chromatography (HPLC), uses various buffers and salts. Trace amounts of these can remain in the final lyophilized (freeze-dried) powder.

• Elemental Impurities: Trace amounts of heavy metals or other elements that may have been present in the raw materials or from the reaction vessel can be present.

• Contaminating Peptides: In some rare cases, if multiple peptides are synthesized in the same facility, cross-contamination with a small amount of a completely different peptide can occur.

If ever people wondered if all these resellers are getting their supply from the same manufacturer, wonder no further.

If one seller has Triz that fails testing, guaranteed other resellers will have Triz in the same dose range fail the same tests. This is because most of these sellers get their wholesale products from the same manufacturer.

 

[Labcat]

Wow, that's a tad extreme.

Probably can’t kill yourself with a failed vial though.

 

[Labcat]

I had questions about what was left in the remainder of the ~1% that isn't peptide on test results. Got this from Gemini and thought it was interesting:

Process-Related Impurities

These are non-peptide materials that remain from the reagents and solvents used during the synthesis and purification steps.

• Residual Solvents and Reagents: The final step of peptide synthesis often involves cleavage from the solid support using strong acids like Trifluoroacetic Acid (TFA). Some of this acid remains, and the peptide is often isolated as a TFA salt. Other solvents or scavengers (reagents used to prevent unwanted side reactions) can also remain.

• Salts and Buffers: The purification step, typically High-Performance Liquid Chromatography (HPLC), uses various buffers and salts. Trace amounts of these can remain in the final lyophilized (freeze-dried) powder.

• Elemental Impurities: Trace amounts of heavy metals or other elements that may have been present in the raw materials or from the reaction vessel can be present.

• Contaminating Peptides: In some rare cases, if multiple peptides are synthesized in the same facility, cross-contamination with a small amount of a completely different peptide can occur.

Can you ask that AI for more detail? A list of those buffers and salts for solid phase synthesis would be nifty and useful.

 

[fatty33]

Sterility fails are very very common. That's why most people don't test for them, why there are so many conversations about using Bac water and so many conversations about filtering.

Thanks I had no clue

 

[Calm Logic]

Yeah, I was surprised too, but a quick look at TG shows failures from SRY (rest in peace) and elsewhere. Not to mention previous NAD from Nexaph, which I posted about a while ago.

Makes me want to switch to pens, which would motivate me to filter since you are taking it out of the vial anyway.