I gave the steps in my previous post. You mix everything in the original vial, add 2ml BAC, test PH, add a a few units of SB, test, continue adding SB and testing until you get to PH=6, then add remaining BAC water. Just keep track of the amount of SB you add and subtract it from remaining 1ml of BAC.
For example add 2ml of BAC, and it takes .4ml of SB to bring it to PH6, then add .6ml of BAC to make the entire vial 3ml.
Also, make sure you add a vent to NAD+ vial (using a luer lock needle or an insulin syringe with the plunger removed), The SB will cause the NAD+ to bubble up and you need to let the gas escape.
You should only need to experiment (add SB test, add more SB test) for the first vial. Subsequent vials in the kit should need the same amount of SB to bring them to PH6.
Hi @GimmeABreakI gave the steps in my previous post. You mix everything in the original vial, add 2ml BAC, test PH, add a a few units of SB, test, continue adding SB and testing until you get to PH=6, then add remaining BAC water. Just keep track of the amount of SB you add and subtract it from remaining 1ml of BAC.
For example add 2ml of BAC, and it takes .4ml of SB to bring it to PH6, then add .6ml of BAC to make the entire vial 3ml.
Also, make sure you add a vent to NAD+ vial (using a luer lock needle or an insulin syringe with the plunger removed), The SB will cause the NAD+ to bubble up and you need to let the gas escape.
You should only need to experiment (add SB test, add more SB test) for the first vial. Subsequent vials in the kit should need the same amount of SB to bring them to PH6.
Many thanks, i buffered my NAD+ with baking soda and the burning pain was gone.
Quick question: Is there any research done on how long we can store a buffered NAD?