There was a big discussion about this and the 5 mL vials would have caused production changes, and that would have increased the GB costs more than they were willing to bear. I trust this manufacturer more than most. I'm going filter mine into 5 mL vials just to make it more manageable.
Peptide filtering
- Thread starter DonkeyTrams
- Start date
woundcarping
GLP-1 Specialist
Filtering into a new 5ml vial costs $3 in materials... you're tilting at windmills, which is absolutely fine, but expect loneliness.
Lilly Direct ships at 30mg/ml for the 15mg dose vials... not sure where you're getting the 25mg/ml figure.
Yes, I was part of that discussion. I thought about buying a kit just to run an advanced panel on it, the one offered by Uzorak. But that would make it too expensive for me.
The 30mg/mL is for tirzepatide, and Lilly is not selling retatrutide yet.
woundcarping
GLP-1 Specialist
Right.... so where is Lilly saying Reta is limited to 25mg/ml?
Clinical trials. Just like for tirzepatide trials, pens had a max volume of 0.5ml, which puts solubility for tirzepatide at 30mg/ml max, and retatrutide at 24 max.
BT labs could also run the panel and there is a discount code. I thought Alex was running a special also, since his lab is relatively new. Could be wrong.
Do you have a link to a btlab COA for advanced analysis ? First time I'm hearing about it. For reference, here's the one from Uzorak https://uzorak.com/#/verify/CZ06ZL
They use both hplc-uv and hplc-ms for the analysis.
Sent you a DM
RetCurious
GLP-1 Enthusiast
Have you tried their product before?
A's interview by K was impressive as were the manufacturing vids. been wanting to try them since. A full kit of R140 is more than I can fathom, I'd like to get a single to play with if anyone wants to break one out for sale.
I just got some Mots from them. Haven't tried it yet, but their testing and reports are good. I like that A does the entire process. My Reta is going right into the bomb shelter to wait for the peptocalypse.
woundcarping
GLP-1 Specialist
What do you reckon a single is worth? You can DM if that's more comfortable.
I always filter because there's no reason not to. You lose a little volume and you can get some of that back with an air purge or extra bac water, but you also filter out bacteria and other debris that might be in your vial that you can't properly see.
Yeah, Mr measuring in grams guy. That's the ticket.
woundcarping
GLP-1 Specialist
I thought I heard something knocking...
RetCurious
GLP-1 Enthusiast
One account has 20 kits ordered. 😲
randompersonrandom
GLP-1 Specialist
The r140 in 3ml vials thing is kind of wild to me. Like, this seems like such a cheap problem to solve, and it takes two bucks:
3ml of bac water into the vial. Let it dissolve til it's either dissolved or mostly dissolved. Pull it into a syringe, and transfer it to a 5ml vial.
Put 2 more ml into the original vial. Swirl it around a bit to get anything that's in it, then transfer that to the 5ml vial too.
Let it all dissolve well in the 5ml vial, then filter THAT into either a new 5ml vial, or your aliquots.
Be happy.
Like...I don't fully understand why this is a big deal.
3ml of bac water into the vial. Let it dissolve til it's either dissolved or mostly dissolved. Pull it into a syringe, and transfer it to a 5ml vial.
Put 2 more ml into the original vial. Swirl it around a bit to get anything that's in it, then transfer that to the 5ml vial too.
Let it all dissolve well in the 5ml vial, then filter THAT into either a new 5ml vial, or your aliquots.
Be happy.
Like...I don't fully understand why this is a big deal.
HereKittyKitty
GLP-1 Enthusiast
I do filter the vent needle. Let's be super generous with the alcohol swabs and say it's $3.75. I'm worth that much per vial. I'm still a far cry from $500/month with all supplies and the teeny tiny vial on my counter.
BNLFL
GLP-1 Specialist
That's my plan. I have a kit ordered. It'll be a while till it's here.
Same plan here.
randompersonrandom
GLP-1 Specialist
What concerns? I plan to do that; 3ml to mostly-dissolve, move, then 2 ml back into the initial vial, rinse, move rinse to new vial. Then it's 5 ml to 140 which should be fine; unless I chicken out and do two rounds of 3ml into a 10ml vial, which I might.
BNLFL
GLP-1 Specialist
Messing with the calculator. I looked at 6ml total in a 10ml vial for a 6mg shot at 26 units. That's just about 24 shots. That would be 3 months work for my wife and I at 6mg each once a day staying at the current 6mg. I think I did that right. I'm curious what the total fill amount will be.
BNLFL
GLP-1 Specialist
I could get away with the 90 days. We have a couple months easy to figure it out.
Having been on the bad end of a Reta batch that ended up failing sterility tests, I started down the rabbit hole of filters. I have been filtering using "sterile" 22 micron filters since I started researching peptides to help my wombat, but on researching what to do about this most recent test failure I realized that all filters are not made equally.
I found that the pass/fail that janoshik gives for sterilization tests is not enough information to just move on, filter, and use failed peptides (not that I would, I'm just researching facts) and that the amount of "stuff" that a filter can clean up is limited - the filter can be overwhelmed, so you need need to know the level of failure for any sterility test if you were to theoretically want to remediate the contamination.
Also I now understand that "normal" 22u filters are not up to the standard of "sterilization grade" filters and are perhaps not enough to give me the safety I'm looking for. The ones I found most commonly listed are the PureFlo D65 Disc Filter 0.65um / 0.2um - Sterile Compounding (Aqueous), but I'm having trouble finding these high grade filters.
I found that the pass/fail that janoshik gives for sterilization tests is not enough information to just move on, filter, and use failed peptides (not that I would, I'm just researching facts) and that the amount of "stuff" that a filter can clean up is limited - the filter can be overwhelmed, so you need need to know the level of failure for any sterility test if you were to theoretically want to remediate the contamination.
Also I now understand that "normal" 22u filters are not up to the standard of "sterilization grade" filters and are perhaps not enough to give me the safety I'm looking for. The ones I found most commonly listed are the PureFlo D65 Disc Filter 0.65um / 0.2um - Sterile Compounding (Aqueous), but I'm having trouble finding these high grade filters.
I am not sure if this is intentional, but you seem to be confusing the pore size for the common filters.
It's possible you bought 22 micron filters, but the most common size I have seen used, and the filters I buy from biologix, are 0.22um.
See the peppys thread about solubility and aggregation concerns even after dilution.
randompersonrandom
GLP-1 Specialist
damnit, you're gonna make me have to join peppy's
ilateapex
GLP-1 Novice
🚫No Source Discussion🚫
I am new to this and trying to understand this process. I understand you to say you put 1ml in to the original product vial and reconstitute it. Then filter it and transfer to a new vial. Then add another 1ml plus the 15 units for the filter back into the original vial and "rinse" the vial. This is then removed, filter and added into the new vial. What is the sequence of barrels and needle changes? Do you use the same barrel and needle for adding the BAC water sticking it into the two vials twice or is it changed? Do you just leave the needle in the new vial with filter and connect the "rinse" mix? I think I understand the process but not sure on when to use new needles and barrels.
Also, I am on par with your costs of materials expect for the filters. I am about twice that in cost at $1 each. How do you get to $0.51 per filter?
I know you weren't asking me, but for filters, this is how.
Biologix® 13mm Sterile PES Syringe Filter, 0.22µm, 100/pk
High-throughput Biologix® sterile PES syringe filters. Ideal for biological fluid filtration. >98.5% protein recovery. Multiple diameters. Shop now!
biologixusa.com
BNLFL
GLP-1 Specialist
Did you buy any of the 140? I didn't see your name here, on there. Different name maybe?
This is how I do it.
I use a needle and a 3ml syringe, draw 3ml BAC water, inject that into the lyophyllized powder.
Once reconstituted (roll it around gently on the counter for a few minutes), I draw that into the syringe. Leave that needle, disconnect the luer lock syringe.
Attach a filter to the syringe. Affix a fresh needle. stab that into your cartridge (if you use pens), or a sterile vial. Push the contents of the syringe thru the filter, into the cartridge/vial. Go slowly, there is usually some resistance. (note if you're using a pen cartridge, stab a cheap diabetic needle in the top as a vent, as well, or you'll push the plunger out the bottom when filling it).
Once all the contents are in the cartridge/vial, back the needle out so it's in the air gap in the vial, and release the needle. Even using 13mm filters, there will be some resistance and the plunger will spring back a few units. This way what gets drawn back from the cartridge/vial is air and not valuable product.
Disconnect the syringe from the needle. Draw in a few units of air. Reconnect, and push this thru the filter as well. This is the "air flush" to recover some of the liquid remaining in the filter.
If you'd like, you can use a 3rd needle, and push a few ml of BAC water thru the filter instead. I don't bother. You could also pre-wet the filter before use (use a cheap diabetic syringe to grab a few ml BAC water and just rinse it into the filter before use. I also don't bother).
As V6R3W said, buying Biologix filters in bulk they are $.51/ea. Needles are $.10-.15 each. Even with the cartridge or vial, it only costs about $1 to filter.
I like your screen name!
