Putting Air in the syringe and into your vial.

[Whiynot20026]

To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill

 

[dgoldrich]

I put air in my vials every time...never had an issue

 

[quicksilver80]

Some of these vials have such a hard vacuum id lose weight from waiting to draw a dose and otherwise unable to use my hands for anything else. Be old and grey by the time that shits done with a 31g needle lol

 

[tcpnomad]

I wonder how it even works for you then?! I mean, the vials have a vacuum in them. Is it hard to get the peps out?

 

[Whiynot20026]

I wonder how it even works for you then?! I mean, the vials have a vacuum in them. Is it hard to get the peps out?

I have no issues at all. The 31s are slow. The 30s are better. It drips in like an IV. But I’m doing super small doses currently. Nothing over 3mg. I could easily see doing a large dose, and adding air as a benefit for sure.

Bill

 

[bada bing]

That's the purpose of the benzyl alcohol in the BAC. It stops any bacteria growth for a reasonable duration of usage after reconstitution.

I usually inject the same amount of air as I'm intending to pull out.

 

[BNLFL]

I put air in my vials every time...never had an issue

Same here.

I wonder how it even works for you then?! I mean, the vials have a vacuum in them. Is it hard to get the peps out?

Not all do, and not when you filter into a new vial. I normally vent, so no pressure at all. Pushing the air in lets you draw the product into the injection syringe. Drawing into the vials with a 31g is slow enough.

 

[tcpnomad]

I normally vent, so no pressure at all.

Well, that's putting air into the vial 😛.

 

[eidos]

To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill

Yes, it can.

Mainly Pseudomonas bacteria in the air (against which benzyl alcohol has little effect). Staph that shed from your skin, body hair/hair. Possibly a few fungal spores from you and your clothes. Above all, the entire microbiome surrounding you within a radius of about one meter, which you release with every exhalation. Enough? ;-)

That’s why you need to thoroughly disinfect your hands or wear alcohol-treated gloves, remove watches and jewelry, wear a mask (I still have plenty from the COVID...), and work in a clean, windless environment (nowadays, I have to turn off the AC and fans, and with it being 40°C outside, that’s tough!).

Watch nursing training videos to learn best practices.
Definitely avoid random YouTube videos (especially PepCritic; I just watched one, and it made my skin crawl how badly he does it!).
I prepare the recons in batches in pen vials. The venting problems you have don't occur when injecting with a pen. It's really a consequence of the multi-dose in a vial.
I put a hairnet on my head and a non-woven lab coat during recons (not during injections). They're really inexpensive, and you can use them for a while: when you're working alone, there's no risk of cross-contamination. Just, watch out for the cat!

You can use a filter attached to the end of a vent needle to equalize the pressure. This filter can be reused for a while if you store it properly. PTFE is good for air, but PES is okay too. 0.1µm PTFE if you’re really paranoid ;-) Even paranoids can have enemies: Pseudomonas and Staphylococcus are nasty.

 

[mybodyisasewer]

Yes, it can.

Mainly Pseudomonas bacteria in the air (against which benzyl alcohol has little effect). Staph that shed from your skin, body hair/hair. Possibly a few fungal spores from you and your clothes. Above all, the entire microbiome surrounding you within a radius of about one meter, which you release with every exhalation. Enough? ;-)

That’s why you need to thoroughly disinfect your hands or wear alcohol-treated gloves, remove watches and jewelry, wear a mask (I still have plenty from the COVID...), and work in a clean, windless environment (nowadays, I have to turn off the AC and fans, and with it being 40°C outside, that’s tough!).

Watch nursing training videos to learn best practices.
Definitely avoid random YouTube videos (especially PepCritic; I just watched one, and it made my skin crawl how badly he does it!).
I prepare the recons in batches in pen vials. The venting problems you have don't occur when injecting with a pen. It's really a consequence of the multi-dose in a vial.
I put a hairnet on my head and a non-woven lab coat during recons (not during injections). They're really inexpensive, and you can use them for a while: when you're working alone, there's no risk of cross-contamination. Just, watch out for the cat!

You can use a filter attached to the end of a vent needle to equalize the pressure. This filter can be reused for a while if you store it properly. PTFE is good for air, but PES is okay too. 0.1µm PTFE if you’re really paranoid ;-) Even paranoids can have enemies: Pseudomonas and Staphylococcus are nasty.

Posts like this are why I'm here but also make me wonder how I am not dead.

I don't know how much of that is realistic for me to implement, but I recently started using caps for the reconned vials and I wipe those out with an alcohol swab. I like the mask idea I will probably start doing that.

Now I need to look up vent needles.

 

[Whiynot20026]

Yes, it can.

Mainly Pseudomonas bacteria in the air (against which benzyl alcohol has little effect). Staph that shed from your skin, body hair/hair. Possibly a few fungal spores from you and your clothes. Above all, the entire microbiome surrounding you within a radius of about one meter, which you release with every exhalation. Enough? ;-)

That’s why you need to thoroughly disinfect your hands or wear alcohol-treated gloves, remove watches and jewelry, wear a mask (I still have plenty from the COVID...), and work in a clean, windless environment (nowadays, I have to turn off the AC and fans, and with it being 40°C outside, that’s tough!).

Watch nursing training videos to learn best practices.
Definitely avoid random YouTube videos (especially PepCritic; I just watched one, and it made my skin crawl how badly he does it!).
I prepare the recons in batches in pen vials. The venting problems you have don't occur when injecting with a pen. It's really a consequence of the multi-dose in a vial.
I put a hairnet on my head and a non-woven lab coat during recons (not during injections). They're really inexpensive, and you can use them for a while: when you're working alone, there's no risk of cross-contamination. Just, watch out for the cat!

You can use a filter attached to the end of a vent needle to equalize the pressure. This filter can be reused for a while if you store it properly. PTFE is good for air, but PES is okay too. 0.1µm PTFE if you’re really paranoid ;-) Even paranoids can have enemies: Pseudomonas and Staphylococcus are nasty.

Wow,

You do what I see in some YouTube videos. That’s great. I do alot of things you recommend. So far it has been a very sterile environment in my home.

Bill

 

[Mochizuki0124]

For my first few pulls I usually add air but around my 3rd or 4th pull usually its no longer necessary.

So basically I do both.

 

[sheilarae74]

I do it and never had any problems. Maybe if I was immune compromised I wouldn't.