Sterilizing injection pen cartridges

[PickleRN]

These are just my most educated guesses - as far as the difference in temperatures between food and the sterilization of injectable tools, it comes down to the fact that when you inject something you are bypassing all of the body’s natural defense systems - skin, stomach acid, enzymes, and the immune system in your gut.
The most common form of bacteria seen in wounds is staphylococcus. If you maintain excellent hand hygiene, use alcohol swabs before you pin and keep a dedicated area that is clean you’re doing good. As for sterilizing the cartridges, I would agree with everyone else who said that it’s a good idea to make sure that they are sterilized.

 

[PickleRN]

FWIW, we have a test group going on right now at FPPG Test Group that is testing 4-5 various vendors' pen cartridges if anyone is interested.

I am interested

 

[Jolly Molly]

I am interested

Can I share links here or DM you? I don't want to break group rules.

 

[scarywood75]

Can I share links here or DM you? I don't want to break group rules.

It's a paid test, you still want the link?

 

[Jolly Molly]

It's a paid test, you still want the link?

LOL, I made the testing group. I've got the link. I was asking about sharing it with PickleRN.

 

[Mr. Smith]

Just curious. How does this affect the rubber. I got some recently from Heaven's store Aliexpress. The rubber stoppers seemed very difficult to move. It almost takes brute force to get them started. I purchased others from EZPENS that uses steam sterilization and those rubber stoppers move very nicely, very smooth.

I think just applying heat, would dry the rubber out? The steam process wouldn't do that. Rubber is a pesty product when it comes to exposure to heat and UV. Just look at what happens to the rubber parts of your car.

 

[Sam90078]

Suprised with the instapot conversation noone has share this study again

Assessment and verification of commercially available pressure cookers for laboratory sterilization - PMC

Laboratory science requires careful maintenance of sterile reagents and tools as well as the sterilization of waste prior to disposal. However, steam autoclaves typically used for this purpose may not be readily accessible to everyone in the ...

pmc.ncbi.nlm.nih.gov

They talk about a few different presure cookers and the

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Instant Pot​

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IP-DUO80​

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In this work, we assessed the viability of four brands of 8-quart pressure cooker to sterilize biological consumables and instrumentation. All four brands inactivated the microbes contaminating microbiological culture media and on metal implements within 15 mins and 30 mins of run time, respectively. The pressure cookers tested also inactivated high titers of bacteria and fungi, some of which are medically relevant strains, within 15 mins and 60 mins of run time, respectively. Unlike the other brands tested, the Instant Pot was capable of inactivating 106 G. stearothermophilus spores, which indicates that it can sterilize items to the level generally deemed acceptable for laboratory autoclaves. These data demonstrate that the pressure cookers tested are a viable alternative for steam sterilizing laboratory items when an autoclave is unavailable.

 

[Arnie]

Just curious. How does this affect the rubber. I got some recently from Heaven's store Aliexpress. The rubber stoppers seemed very difficult to move. It almost takes brute force to get them started. I purchased others from EZPENS that uses steam sterilization and those rubber stoppers move very nicely, very smooth.

I think just applying heat, would dry the rubber out? The steam process wouldn't do that. Rubber is a pesty product when it comes to exposure to heat and UV. Just look at what happens to the rubber parts of your car.

This thread was very useful. My labels were blue initially. I had a prestige pressure cooker with weight and steamed these for half an hour and the labels turned black. A tiny drop of water was inside the pouch after this, but thats ok as the stopper did not pop out. As far as the stopper being stuck is concerned, I guess its some kind of glue or seal and is intended to be there. I tested one of them before steaming and it was still stuck. I think it surves 2 purposes. To prevent the stopper from popping when steamed. To accidently prevent the Stopper popping out when filling medication without using a second needle to let the air out, and its definitely not steam doing it.

Also I dont know what temperature the pressure cooker went as the bosch infrared thermomenter could not read the temp due to reflective surface of stainless steel. Hope this helps.

 

[scarywood75]

We have a testing group where I participated to test a ton of different carts for sterility, some already been sent to Jano

 

[Tilted Halo]

FWIW, we have a test group going on right now at FPPG Test Group that is testing 4-5 various vendors' pen cartridges if anyone is interested.

in interested if this is still going on, thx

 

[scarywood75]

in interested if this is still going on, thx

No longer going on, but test results will be published at due time

 

[Morbius]

Old thread but...

Proper autoclaves run at 15 PSI but it is possible to sterilize at lower pressure and temperature if you do it longer.

This paper tested the Instant Pot and found that it was able to sterilize 1L of liquid media effectively when run for 150 minutes. Scroll down to "Instant Pot pressure cooker passes the spore sterilization test."

Assessment and verification of commercially available pressure cookers for laboratory sterilization

Laboratory science requires careful maintenance of sterile reagents and tools as well as the sterilization of waste prior to disposal. However, steam autoclaves typically used for this purpose may not be readily accessible to everyone in the scientific community, such as K-12 teachers...

journals.plos.org

These data indicated that the Instant Pot pressure cooker could sterilize spores to the level of an autoclave, and we infer that it would be capable of inactivating all other less heat resistant organisms to properly sterilize laboratory items for research.... All four pressure cookers tested were able to inactivate the microbes present in all of the trials except for the inactivation of G. stearothermophilus spores, in which only the Instant Pot was able to produce steam, pressure, and heat to the level necessary to achieve sterilizing conditions.

Now actually properly treating any particular kind of vial is a different question. (If it is sealed, steam doesn't get inside. If it isn't sealed, how do you assemble it in a sterile manner?) But in general it should be possible to sterilize gear and media in a 12 PSI Instant Pot.

Would I rather have 15 PSI, yes. In fact I bought a 15 PSI Presto pressure canner for my mushroom cultures.

 

[FlowerFairy]

The instapots only get up to 12psi. They are used but you need to increase the run time. I would go 2 hours minimum. The newer instapot max that does 15psi should be ok but I never researched how well they maintain pressure / temperature.
However I would sooner use one of the cheap 6 quart pressure cookers with no gauge but does have a traditional rocker weight that I can manipulate.

Someone on STG has been using an instapot - she said there is one model that gets high enough. They have a pinned post about it somewhere