The temperature journey of a peptide

[atomicjake]

From the point of its creation to arriving at your door and beyond.

I can only assume that peptides are made at room temperature?
Then freeze dried? ( Lyophilised )
Stored at room temperature for an unknown amount of time?
Shipped at room temperature until it arrives at your door.
Then 9/10 are frozen.

When I say room temperature, I mean they're not in a temperature controlled environment.

So is there any point or is it even detrimental to freeze a Peptide in its powder form if it's going to be used within a few months.

Is it best to only freeze a Peptide if it's not going to be used for 6+ months ?

Anyone with any insights ?

 

[ostrichsak]

I believe the idea here is to reduce the oxidation rate. Granted, this is a very very very low rate once it's lyophilized but it's an oxidation rate none-the-less. The idea of freezing is to dramatically reduce this rate of oxidation and thus prolonging the overall efficacy window of the peptide.

Refrigerating peptides once they're reconstituted is to slow the rate of any bacterial growth that may exist from the reconstitution process. Try as we might, there may still be some small amounts of foreign bodies present and allowing them to sit at room temperature in a liquid would allow them to grow at a much quicker rate to potentially reach a dangerous amount before we're able to use them up.

For me, it's not a big deal to just freeze up the lyophilized vials to keep their efficacy as high as day one for years, possibly decades and refrigerate the reconstituted vials and try to use them up within a month or so of reconstitution. Seems best practice. I even have some cool storage solutions for each location to help keep them safe and organized.

If you want metrics to assign to those concepts I outlined I don't have any although I'm sure they exist somewhere. The logic checks out though and I see no real reason not to follow these commonly accepted best practices. If you have a pressing reason, well, then things kind of fall into the realm of your own comfort levels.

 

[atomicjake]

Thank you.

Would there be any degradation during the freezing / thawing process?

 

[zpped]

Thank you.

Would there be any degradation during the freezing / thawing process?

If you are talking about "freezing/thawing" the lyophilized (powdered) peptide... that doesn't happen when you put it in a freezer or take it out. There is no liquid in the vial so there is nothing to freeze.

 

[YoYoFat]

Jano tested peps that had been in his garage for 10 years and saw little degradation. I’m not terribly worried about room temps as they’re made, stored, and transported to me, but I do freeze them upon receipt.