I’m so confused now because I was residng last night and Peter Magic said the mass they report on the typical peptide analysis is not the net peptide content, because we don’t need it.
He also said the purity only means the percent of the desired peptide among the peptide/fragments, NOT what %of the vial contents is your peptide, how do we know how much medication is actually present? (The problem is that lyophilized peptide has the counterion, so if we weigh the puck, it’s not all med. The counterion and residual solvent is not identifiable using the hplc method he uses, he said.)
Related, I’m also now unsure whether or not fillers are in the puck, for tirz or other peptides. I have read that everyone puts it in and also that they would be identified inpurity tests, that the fillers stabilize the lyophilate and make the medication even visible, but is any of that true?
Wiser heads, what say you?
He also said the purity only means the percent of the desired peptide among the peptide/fragments, NOT what %of the vial contents is your peptide, how do we know how much medication is actually present? (The problem is that lyophilized peptide has the counterion, so if we weigh the puck, it’s not all med. The counterion and residual solvent is not identifiable using the hplc method he uses, he said.)
Related, I’m also now unsure whether or not fillers are in the puck, for tirz or other peptides. I have read that everyone puts it in and also that they would be identified inpurity tests, that the fillers stabilize the lyophilate and make the medication even visible, but is any of that true?
Wiser heads, what say you?
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