Where is everyone getting acetic acid?

[Bflo_Bflo_Bflo_Bfl]

So I’m looking at the fibrils threads and I’m wondering - where are you getting acetic acid and what type are you using? Left to my own devices I’d inject distilled white vinegar into a vial of BAC, bit by bit, and test pH as I went along.

Is there a better source of acetic acid / a better way to do this? I’m honestly not too concerned,but if it’s easy enough to get a low pH reconstitution solution I might as well err on the side of caution.

 

[Peotidethrowaway]

Think I got mine from sunny solutions or someone similar. I’ve never used it tho

 

[mopo89]

i think most of us just reconstitute normally with bac water.

 

[TooBigtoFail]

With AA at 0.6% concentration, I use 75% AA and 25% bac for reconstitution. Vinegar is not USP, not filtered for sediment, etc. If your vinegar is 6% AA, then maybe a few drops in 1ml of bac, test the pH, and then filter it.

I googled and found many AA 0.6% suppliers. Carolina.com for lab supplies. Many pep sites selling 0.6% AA.

 

[Ted R]

Think I got mine from sunny solutions or someone similar. I’ve never used it tho

Got mine from UndergroundSupply.shop

 

[Nugget01]

I got mine from here - https://ozvials.com.au/products/bacteriostatic-water-20ml
(Australia)

Haven't used it yet though and I'm on my second week of cagri

 

[Wecandothis]

So I’m looking at the fibrils threads and I’m wondering - where are you getting acetic acid and what type are you using? Left to my own devices I’d inject distilled white vinegar into a vial of BAC, bit by bit, and test pH as I went along.

Is there a better source of acetic acid / a better way to do this? I’m honestly not too concerned,but if it’s easy enough to get a low pH reconstitution solution I might as well err on the side of caution.

Sunny solutions https://form.jotform.com/252414058048151

 

[clayd]

So I’m looking at the fibrils threads and I’m wondering - where are you getting acetic acid and what type are you using? Left to my own devices I’d inject distilled white vinegar into a vial of BAC, bit by bit, and test pH as I went along.

Is there a better source of acetic acid / a better way to do this? I’m honestly not too concerned,but if it’s easy enough to get a low pH reconstitution solution I might as well err on the side of caution.

months ago i tried to go the bac water + vinegar route with a vial of cag and it immediately went cloudy. the vinegar denatured the peptide, this is the same as when your liquid in recipes goes cloudy when an acid is introduced. the cag is then destroyed and can't be salvaged.

i don't know why it did that, i did the math several times, and vinegar is literally acetic acid, so i would have thought it would work the same, but it didn't. just fyi, trying to save you from ruining that cag

 

[clayd]

here's my post on the vinegar https://glp1forum.com/threads/i-tweaked-the-ph.2200/post-35243

 

[Bflo_Bflo_Bflo_Bfl]

here's my post on the vinegar https://glp1forum.com/threads/i-tweaked-the-ph.2200/post-35243

Thanks a lot, that’s good to know. Did you premix the BAC and vinegar, or put one in the vial and then the other?

I wound up just using BAC, at least to get started. So far cagri has been amazing. It’s like when I first started sema.

I bought two vials from one of the individual vial vendors to get started. If I manage to get a kit before I get into the second vial, maybe I’ll try vinegar just to see what happens - but I might wind up needing it so I’ll have to wait and see.

 

[RocketGotRobbed]

So I’m looking at the fibrils threads and I’m wondering - where are you getting acetic acid and what type are you using? Left to my own devices I’d inject distilled white vinegar into a vial of BAC, bit by bit, and test pH as I went along.

Is there a better source of acetic acid / a better way to do this? I’m honestly not too concerned,but if it’s easy enough to get a low pH reconstitution solution I might as well err on the side of caution.

Atomik Labz

 

[Bflo_Bflo_Bflo_Bfl]

Atomik Labz

Yeah, some earlier replies to my post mentioned sources.

I played around with vinegar and BAC with an old bottle of BAC. It took just a tiny amount of vinegar to lower the pH. I wound up reconstituting with plain BAC for now. Now that I found out that cagri works for me, I guess I’ll have to check out some suppliers and get some low pH solution.

 

[Bflo_Bflo_Bflo_Bfl]

Atomik Labz

Sorry, got confused and replied to the wrong post with that last one.

 

[diogenes]

Just using vinegar or glacial acetic acid seems insufficient to me. Yes, you might hit pH 4.0, but that pH isn't stable. Adulterants like CO2 from the air or impurities on the needle could throw it enough to matter.

My instinct is to add some biocompatible buffer. They use sodium acetate in the solution for pramlintide. Is there a reason cagri users aren't buffering with sodium acetate or similar?

 

[DFW007]

the 1 from amazon worked just fine.

 

[ocuzuc]

Just using vinegar or glacial acetic acid seems insufficient to me. Yes, you might hit pH 4.0, but that pH isn't stable. Adulterants like CO2 from the air or impurities on the needle could throw it enough to matter.

My instinct is to add some biocompatible buffer. They use sodium acetate in the solution for pramlintide. Is there a reason cagri users aren't buffering with sodium acetate or similar?

I know, right? As the reason to use acetic acid instead of HCl/NaOH to adjust to pH is for an acetate buffer over a phosphate one. But if you’re using your cagri vial in under one month, with 4 injections how much does the pH wander? And how much does it even matter? Meaning what’s the degradation? I’m not sure we know. But I see people are also adding lidocaine to it too. Lidocaine (at least the Hospira) has both hydrochloride acid and sodium hydroxide to make its pH 6.5-7. And here acetic acid + sodium hydroxide -> sodium acetate + water.

On a fun thought, I see people using 8.4% sodium bicarbonate sterile water for injection to reconstitute NAD+. So a cagri on top of a NAD+ and you’ll get sodium acetate + water + carbon dioxide gas — the typical home volcano chemistry experiment of adding vinegar to baking soda. lol.

 

[diogenes]

The issue isn't that the cagri degrades.

It's that it aggregates into fibrils, which we know to be toxic. We don't have published data on exactly how sensitive that process is to pH, or how it varies with time/temperature. We know it happens less at pH 4 than at neutral pH.

So IMHO it makes sense to do what the drug companies do, and use a buffered pH 4 solution.