Is it feasible to split a reconstituted peptide into two vials?

[Mara_aa]

Hi everyone,
sorry if this has already been covered, I tried searching but couldn’t find anything, so apologies if I missed it.
Also, I should say upfront that I already have very strong doubts that my question might be a bit silly, but here we go anyway.
I have a vial of Tirz that needs to be reconstituted, and I would like to split the final solution into two separate vials (50/50).
Is this actually feasible without affecting sterility or integrity?
Any advice regarding sterility, handling, or other would be really appreciated.
Thanks a lot! 🫶

 

[FRMN01]

You need to get sterile sealed vials. Reconstitute as usual, then draw half and inject into new sterile vial.

 

[ambot88]

Yes you can, you can get sterile vials from a medical store or amazon and once you recon the original vial just pull half out and put in the new vial. I do it frequently, I share with my sister so I make it up and then put her half in a fresh vial.

 

[indolent]

Hi everyone,
sorry if this has already been covered, I tried searching but couldn’t find anything, so apologies if I missed it.
Also, I should say upfront that I already have very strong doubts that my question might be a bit silly, but here we go anyway.
I have a vial of Tirz that needs to be reconstituted, and I would like to split the final solution into two separate vials (50/50).
Is this actually feasible without affecting sterility or integrity?
Any advice regarding sterility, handling, or other would be really appreciated.
Thanks a lot! 🫶

It's called aliquot. I wouldn't touch it with a ten foot pole due to sterility fears (even when the marketer of the empty vial claims "sterile"). But plenty of smart people here do so.

What I do, though (among other off-label things) is keep using a refrigerated vial of reconstituted tirzepatide for up to 3 months. That assumes vigilant swabbing of the top with alcohol every time, including at recon. And it assumes you're using bacteriostatic water ... the real stuff (Pfizer Hospira), or otherwise from a very select few group buy orgs that test them. I understand that it's hard to find in Europe, though.

I also readily freeze reconned vials then thaw to begin the 3 months of use.

Your mileage may vary ... you need to set your own risk tolerance as you read and learn more.

 

[Mara_aa]

You need to get sterile sealed vials. Reconstitute as usual, then draw half and inject into new sterile vial.

Oh perfect, I'll make sure to use new sterile sealed vials and handle everything carefully. I really appreciate the help!

 

[Mara_aa]

Yes you can, you can get sterile vials from a medical store or amazon and once you recon the original vial just pull half out and put in the new vial. I do it frequently, I share with my sister so I make it up and then put her half in a fresh vial.

Thank you, that’s really helpful!
Good to know it works well in practice too. I’ll make sure to use proper sterile vials and handle everything carefully. Really appreciate the advice!

 

[Mara_aa]

It's called aliquot. I wouldn't touch it with a ten foot pole due to sterility fears (even when the marketer of the empty vial claims "sterile"). But plenty of smart people here do so.

What I do, though (among other off-label things) is keep using a refrigerated vial of reconstituted tirzepatide for up to 3 months. That assumes vigilant swabbing of the top with alcohol every time, including at recon. And it assumes you're using bacteriostatic water ... the real stuff (Pfizer Hospira), or otherwise from a very select few group buy orgs that test them. I understand that it's hard to find in Europe, though.

I also readily freeze reconned vials then thaw to begin the 3 months of use.

Your mileage may vary ... you need to set your own risk tolerance as you read and learn more.

Thanks for sharing your perspective, I really appreciate it. I completely understand the sterility concerns.
I’m still learning and I’ll definitely have to think carefully about what approach I’m most comfortable with.
Out of curiosity, when you say you freeze the reconstituted vial and then thaw it before starting the 3-month use, have you found that it keeps its effectiveness well?
Really grateful for all the knowledge people share here.

 

[AcadiaPeptides]

It's called aliquot. I wouldn't touch it with a ten foot pole due to sterility fears (even when the marketer of the empty vial claims "sterile"). But plenty of smart people here do so.

What I do, though (among other off-label things) is keep using a refrigerated vial of reconstituted tirzepatide for up to 3 months. That assumes vigilant swabbing of the top with alcohol every time, including at recon. And it assumes you're using bacteriostatic water ... the real stuff (Pfizer Hospira), or otherwise from a very select few group buy orgs that test them. I understand that it's hard to find in Europe, though.

I also readily freeze reconned vials then thaw to begin the 3 months of use.

Your mileage may vary ... you need to set your own risk tolerance as you read and learn more.

You know, its a bit off topic but experimenting with peptides has really opened communication in my marriage.
My lovely wife runs a lab, I work in non-profits. Rarely have we had conversations like. " do you know what aliquot means?" - of course duh.
or "honey, do you have 4mm PES .22um individually wrapped sterile filters?" well yea, I use them every day.
Don't get me started on the side eye from asking basic mg vs ml and concentration questions.

 

[Mara_aa]

You know, its a bit off topic but experimenting with peptides has really opened communication in my marriage.
My lovely wife runs a lab, I work in non-profits. Rarely have we had conversations like. " do you know what aliquot means?" - of course duh.
or "honey, do you have 4mm PES .22um individually wrapped sterile filters?" well yea, I use them every day.
Don't get me started on the side eye from asking basic mg vs ml and concentration questions.

That's amazing 😀
Peptides improving marriage communication was definitely not something I expected to hear.
Having a wife who runs a lab must be the ultimate advantage. The rest of us are here googling things like aliquots, mg vs ml and filter sizes and hoping we don't mess it up!

 

[Labcat]

Yes, and a good idea, so gradual contamination each time you puncture does not get the whole batch. I also suggest filtering on your way into the new sterile vial. I always move my recon into new sterile vials or cartridges and filter (an 13mm PES 0.2 microns syringe filter, can even order from Amazon, placed in between your syringe and the needle, easy peasy) along the way, since in general we do not test for sterility. (Few people get sick or can trace it back to bacteria from a vial, but I always think about how peptides are just mini proteins and thus food for organisms, and bac is helpful but not perfect and doesn’t suppress all organisms, including staph, commonly on skin and thus everywhere)

 

[Mara_aa]

Yes, and a good idea, so gradual contamination each time you puncture does not get the whole batch. I also suggest filtering on your way into the new sterile vial. I always move my recon into new sterile vials or cartridges and filter (an 13mm PES 0.2 microns syringe filter, can even order from Amazon, placed in between your syringe and the needle, easy peasy) along the way, since in general we do not test for sterility. (Few people get sick or can trace it back to bacteria from a vial, but I always think about how peptides are just mini proteins and thus food for organisms, and bac is helpful but not perfect and doesn’t suppress all organisms, including staph, commonly on skin and thus everywhere)

That makes a lot of sense, thank you for explaining it. I hadn't really thought about the contamination risk building up with repeated punctures. Filtering while transferring to a new sterile vial sounds like a good precaution. l'il need to think a bit about the best way to set it up on my side since I'm still very new to all of this, but I really appreciate the advice.

 

[Peotidethrowaway]

I used to do it with 120mg tirz. I’d use pen carts and freezing one

 

[Labcat]

I used to do it with 120mg tirz. I’d use pen carts and freezing one

I’ve been wanting to do carts! Have you frozen carts successfully? I’ve been saving some used ones to test, to see how much air space affects it; I have mild concerns about the plug moving out the back, falling out completely, or possibly causing a little contamination from moving over a non-sterile area (it may be okay if plug is dry and doesn’t move far. So no capillary action wicks anything in.)

 

[Labcat]

That makes a lot of sense, thank you for explaining it. I hadn't really thought about the contamination risk building up with repeated punctures. Filtering while transferring to a new sterile vial sounds like a good precaution. l'il need to think a bit about the best way to set it up on my side since I'm still very new to all of this, but I really appreciate the advice.

Feel free to ask questions here or DM me

 

[indolent]

Thanks for sharing your perspective, I really appreciate it. I completely understand the sterility concerns.
I’m still learning and I’ll definitely have to think carefully about what approach I’m most comfortable with.
Out of curiosity, when you say you freeze the reconstituted vial and then thaw it before starting the 3-month use, have you found that it keeps its effectiveness well?
Really grateful for all the knowledge people share here.

Yes, and a group test verified it [for tirzepatide... each peptide behaves differently. Although a similar test on reta found a bit more degradation, but not so much that it's not a viable tactic.]

See post #100 at https://glp1forum.com/threads/tirz-freezing-defrosting.3850/page-4#post-128833

 

[Mara_aa]

I used to do it with 120mg tirz. I’d use pen carts and freezing one

Oh, That’s really helpful to hear, thank you for sharing your experience. I’m still very new to all of this and learning step by step, I truly appreciate the insight.

 

[Mara_aa]

Yes, and a group test verified it [for tirzepatide... each peptide behaves differently. Although a similar test on reta found a bit more degradation, but not so much that it's not a viable tactic.]

See post #100 at https://glp1forum.com/threads/tirz-freezing-defrosting.3850/page-4#post-128833

That’s really interesting, thank you for sharing. It’s reassuring to hear it was actually verified in a group test. I really appreciate the insight.

 

[Labcat]

Yes, and a group test verified it [for tirzepatide... each peptide behaves differently. Although a similar test on reta found a bit more degradation, but not so much that it's not a viable tactic.]

See post #100 at https://glp1forum.com/threads/tirz-freezing-defrosting.3850/page-4#post-128833

That’s how I interpret that also.

 

[skeptick]

I’ve been wanting to do carts! Have you frozen carts successfully? I’ve been saving some used ones to test, to see how much air space affects it; I have mild concerns about the plug moving out the back, falling out completely, or possibly causing a little contamination from moving over a non-sterile area (it may be okay if plug is dry and doesn’t move far. So no capillary action wicks anything in.)

If you use a vent needle the plug won't move at all. Then again it's ok for the plug to move if you pay attention as it travels nearer the open end of the cartridge... Ask me how I know...

 

[Dilbert4Life]

If you use a vent needle the plug won't move at all. Then again it's ok for the plug to move if you pay attention as it travels nearer the open end of the cartridge... Ask me how I know...

Do you mean using vent needle to remove all the air from the cartridge before freezing? Like priming the cartridge.

 

[MG9550]

Hi everyone,
sorry if this has already been covered, I tried searching but couldn’t find anything, so apologies if I missed it.
Also, I should say upfront that I already have very strong doubts that my question might be a bit silly, but here we go anyway.
I have a vial of Tirz that needs to be reconstituted, and I would like to split the final solution into two separate vials (50/50).
Is this actually feasible without affecting sterility or integrity?
Any advice regarding sterility, handling, or other would be really appreciated.
Thanks a lot! 🫶

I do that for KLOW/GHK-Cu cartridges since heavier dilutions ease the sting of injection. I dissolve in 2 mL of Bac water, filter 1 mL of the reconstituted vial into 2 cartridges and then add Bac water to 3 mL into both cartridges.

Regarding sterility, I work in a Still Air Box that's meant for inoculating mushroom spores. Probably overkill, but as long as you sanitise all your vials and your work area and don't touch the rubber with your fingers after sanitising, you should be fine.

 

[Mara_aa]

I do that for KLOW/GHK-Cu cartridges since heavier dilutions ease the sting of injection. I dissolve in 2 mL of Bac water, filter 1 mL of the reconstituted vial into 2 cartridges and then add Bac water to 3 mL into both cartridges.

Regarding sterility, I work in a Still Air Box that's meant for inoculating mushroom spores. Probably overkill, but as long as you sanitise all your vials and your work area and don't touch the rubber with your fingers after sanitising, you should be fine.

Thanks so much for taking the time to explain your process.
In my case I wasn’t planning any further dilution, just splitting the reconstituted solution 50/50 into two sterile vials, so my main concern is exactly what you mentioned: maintaining sterility during the transfer.

I don’t have a setup like yours (no Still Air Box on my side), but it’s really helpful to know how you approach it, especially if I ever decide to go that route.

Really appreciate you sharing this!

 

[Mara_aa]

Do you mean using vent needle to remove all the air from the cartridge before freezing? Like priming the cartridge.

From what I understood, he means using a vent needle during filling to equalize pressure, so the plug doesn’t move.
When he says it’s “ok if the plug moves”, he’s referring to controlling it so it doesn’t travel too far or reach a non-sterile area.
Not talking about removing all the air before freezing or “priming”, it’s about pressure control during the fill.