Peptide pens, dosage and temperature changes

[DJ _Yus]

After some reading I'm still not clear about the best practice for when getting weekly pin, from my pen that sits on the fridge between doses.
Some people say, to let the reconstituted pep to come to room temperature, and then pin.
Some people say, to keep the pep out of the fridge as little as possible to prevent temperature fluctuations that will cause biodegradation faster. Meaning you should pin fast when taking the pep out of the fridge.

What do you guys think, and what practices do you follow?

 

[SeaGypsy]

I use my pens straight from the fridge then immediately back in they go. Warm /cool cycles cannot be good for the pep. If you feel you need to let it come to room temp do not use a pen, just pull your dose from the vial and put the vial back in the fridge.

 

[desinr-gal]

I use my pens straight from the fridge then immediately back in they go. Warm /cool cycles cannot be good for the pep. If you feel you need to let it come to room temp do not use a pen, just pull your dose from the vial and put the vial back in the fridge.

Totally agree with this. The cold doesn't bother me at all, and mine last for months with no problem. Constant flux in temp change is said to weaken the peptides.

 

[Grogu]

I use my pens straight from the fridge then immediately back in they go. Warm /cool cycles cannot be good for the pep. If you feel you need to let it come to room temp do not use a pen, just pull your dose from the vial and put the vial back in the fridge.

I agree with all of this, but just add that some peptides cause less pain at room temperature and in those cases what you suggest is the best approach.

The only "benefits" I can see of putting a weekly peptide in a pen is the fact that you can expel the extra air from the cartridge. I really like that for some reason. And also not puncturing a vial, pen needles are super sharp. I never thought that just one puncture of a vial stopper would dull a needle, but yup, it does. Otherwise, pens for weekly injections only speed things up by a fraction.

 

[desinr-gal]

I agree with all of this, but just add that some peptides cause less pain at room temperature and in those cases what you suggest is the best approach.

The only "benefits" I can see of putting a weekly peptide in a pen is the fact that you can expel the extra air from the cartridge. I really like that for some reason. And also not puncturing a vial, pen needles are super sharp. I never thought that just one puncture of a vial stopper would dull a needle, but yup, it does. Otherwise, pens for weekly injections only speed things up by a fraction.

It can save a lot of time and Plastic, if you are using the same vial for a longer period of time. My 30mg vials last for 30 pins @ 1mg per pin, every 4 days, that lasts 4 months.
pen needles are much better too.

 

[SeaGypsy]

I agree with all of this, but just add that some peptides cause less pain at room temperature and in those cases what you suggest is the best approach.

The only "benefits" I can see of putting a weekly peptide in a pen is the fact that you can expel the extra air from the cartridge. I really like that for some reason. And also not puncturing a vial, pen needles are super sharp. I never thought that just one puncture of a vial stopper would dull a needle, but yup, it does. Otherwise, pens for weekly injections only speed things up by a fraction.

Yes, that's one of the reasons I love the pen needles, always sharp and smooth. I rarely ever feel one during the stick.

 

[eidos]

You’ll get as many answers as there are people on this forum.
🙂

From a physical chemistry perspective:

• There is no change in the state of the water during reconstitution (from solid to liquid). Therefore, there is no physical stress on the solutes.
• The temperature is in Kelvin in the reaction equations (I’m keeping it simple 🙂, so going from 2°C to 25°C means going from 275K to 298K. That’s less than a 10% change in temperature.
• For GLP-1s, there are a significant number of hydrogen bonds and hydrophobic interactions, which means that at these temperatures, the change in energy (ΔG) remains below 10%.
• An increase in temperature increases solubility and will break up certain hydrophobic aggregates (don’t bother looking: you can’t see them with the naked eye; if you do see them, your solution is spoiled).
• Adsorption onto glass walls is minimal at typical reconstitution concentrations (on the order of 1 per thousand), so it is not a factor in our situations.

In general, avoid temperatures above 25°C. Significant degradation occurs at temperatures above 30°C (deamidations, accelerated oxidation).

There’s no need to bring it up to room temperature. You can stop at around 15°C (you can easily find cases with integrated thermometers). That will be enough to increase solubility and prevent thermal shock, which some white blood cells don’t like (some people are allergic to the cold...).

So, it's fine to warm it up, and while it's not mandatory, it helps a little.

 

[Grogu]

Yes, that's one of the reasons I love the pen needles, always sharp and smooth. I rarely ever feel one during the sick.

Yup, love that about pen needles too. Smooth like butter entering your skin. Who knew that one puncture could dull an insulin needle that much, but it does.