I would steer clear from anything with government ties that your name will be attached to.
You are probably correct. I am just a paranoid libertarian. lol
I am a Nurse practitioner . I contacted the university proteomics department for amino acid sequencing of compounded tirz …. He had never done that … I gave him tirz molecular weight, AA sequence and we discussed how to proceed … my boss let me use our clinic to setup an institutional account with the university. They can do the hplc testing for 300 $ but I am content with AA sequencing for $108… being an NP I am able to order medical supplies easily.. it is helpful …. I have used Janoshik and peptide test too. I double tested a bath with Janoshik and the university to. Similar mg results
Yes anyone could do it….. bought agar paste off of amazon…. Bought Tupperware tray to put the agar plates into and used a seed mat to and bbq thermometer to make the plates 95-99 degrees. Was a little to hot so I put a porcelain plate on the seed mat and the Tupperware on top of it….. easy to do a swab a plate …I learned to do it in my microbiology classes but YouTube is great … but I used sterile water with no preservative to reconstitute the peptide…… I have a microscope around to teach my kids
No I used a small amount left over from a shot I think 0.2 ml … diluted the vial with 1 ml of sterile water. Nothing ever grew……. Once I did a nasal swab and it grew out fungi .. a bit scary
Agree this would be a great thread on its own partly because educating people more on this topic is important before they rush out to buy the supplies with zero understanding of sterile technique and then try to hang a vendor for having a contaminated product when they were the problem.
I sanitize my area with bleach solution . The agar plates can only be open for a couple seconds. Place in ziplock bags. Tupperware in dishwasher and then soaked in bleach. plates stored upside down…… always do a control plate at the same time….. but realistically you could have contaminated the sample when reconstituting too.
Most vendors have at least 1 sterility fail by now from actual labs, so I doubt a diy sterility fail could really change anything there. I doubt people would take diy test failing that seriously.
I don’t trust a pass/ fail sterility test. Also a culture will not grow endotoxins. I would want an actual endotoxin count or bacterial count
Endotoxin contamination would be a risk there is an acceptable limit for sq dosing ….. sq is low risk. I have seen some really bad infections from sq insulin and IV drug use .. mostly severe diabetics reusing needles and iIV drug addicts shooting up dirty water and many years ago bath salts …..
I guess that did seem kinda like a call out on my part, I know you didn't seem to take offense but it seems my comment may have been sort of abrasive. Apologies if so! I'm not always good at watching my tone with text. ♥
How effective do you think filtering would be? I had considered filtering when reconstituting, but knowing my own lack of attention to detail it might be better to do it per dose. My hope is that it's reasonably effective against most scary stuff
A regular 22 micron PES filter will get most bacteria and fungi……: endotoxins will usually pass though it. The more expensive filters that have a “positive charge” will remove endotoxins well but are much more expensive
Hi what vendor did you purchase from?
Purchase what? The filters ? Newstar on Alibaba
Sorry, no I meant the Tirz, I know you said you sent compounded tirz for testing but I was wondering if you used a CN vendor for pure lyophilized tirz or you just order from the compounding pharmacy
