When using peptides. Does everyone just...
- Thread starter JoonyO
- Start date
Peotidethrowaway
GLP-1 Enthusiast
Usually if you can’t find anyone talking about doing something like prefilling their dose and waiting, it means no one does that 😀
MsGizmo
GLP-1 Enthusiast
Not really sure the point of what you are asking so I will give my entire process from start to finish.
I have 10mg vials of semaglutide. I store them in the freezer.
I take out a vial to warm up for 10 minutes or so.
I reconstitute with 2ml of BAC water.
I transfer the contents of the vial into a injection pen cartridge.
I put the cartridge in the pen. (photo of pen assembly below)
Remove excess air from the cartridge.
To do this I waste an needle and dial in "doses" with the pen and hit the button until the air is all gone. I'm sure you could find a youtube video of the process if you are curious.
My dose of sema is 1 mg per week.
I have used a peptide calculator and it tells me that I need 20 units.
On my injection day I attach a needle, set the dial to 20
I jab myself in a squishy spot and push the button.
Remove the needle and dispose of it safely.
When my pen is not in use I keep in it a pencil case in the fridge. I do not pay any attention to temperatures.
There are two things here that I admit are problematic.
#1 - I don't filter. Doing this increases the level of risk I am taking.
#2 - I keep and use reconstituted vials for more than 28 days.
I might start filtering at some point. But as it is now I haven't looked into the process and I don't have the supplies. I will continue to use cartridges for longer periods of time. I don't personally believe that they expire that fast. But that is solely my own opinion with no medical facts to back me up. If it ever becomes a problem then I will only have myself to blame.
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vewdew34
GLP-1 Enthusiast
I never wait, take out the fridge and pin.
jordekorn
GLP-1 Novice
When I take my Dupixant, I let it sit out, it’s a lot of liquid, I let it sit for hours sometimes. When I do peptides I fill, clean up the wrappers, paper towels and alcohol pads, then inject.
Dupixant is safe sitting out for days though(14 days before it’s bad), not sure about peptides.
Dupixant is safe sitting out for days though(14 days before it’s bad), not sure about peptides.
cheaperseeker
GLP-1 Apprentice
I pin straight from the fridge.
The amount drawn into the syringe is so tiny it's going to be close to room temperature by the time you inject it.
LET'S DO THE MATH
Well, let's ask an AI to do the math, because I am lazy and I am not going to look anything up. I did measure a 1 cc insulin syringe at 2.5 g though, and I think it's made from polypropylene. So if we assume the syringe is at 68F and the liquid in the vial is at 40F... When the liquid comes to equilibrium with the warmer syringe, it will rise to about 62F.
That's obviously a highly simplified model but ...
Just inject it.
I don't see the point of waiting, what could be the benefit?
LET'S DO THE MATH
Well, let's ask an AI to do the math, because I am lazy and I am not going to look anything up. I did measure a 1 cc insulin syringe at 2.5 g though, and I think it's made from polypropylene. So if we assume the syringe is at 68F and the liquid in the vial is at 40F... When the liquid comes to equilibrium with the warmer syringe, it will rise to about 62F.
That's obviously a highly simplified model but ...
Just inject it.
I don't see the point of waiting, what could be the benefit?
desinr-gal
GLP-1 Enthusiast
I dont wait. I have never had a problem injecting a cold substance, and In an effort to extend the life of my peps, I complete the process and get them back in fridge asap. Also I am using pens, so the rest is sitting in the pen..
I asked because I've had some really crappy nights when taking tesa. Terrible sleep/insomnia. Only on .4mg and ONLY happened on the first pin of a new vial. But EACH new vial was the same crap night. No issues after initial pin. I was a bit concerned so called and the pa who I got the stuff from suggested that I let it sit out for 20 minutes before using. I've since went to 1mg a couple of weeks ago no issues-no new vial YET either so there's that.
Anyway I was recommended for the tesa because I initially thought trt might explain a couple issues ( one of them lousy sleep ) however blood work came back at 860 so she couldn't prescribe test. She laughed a little when she said it ( I'm coming up fast on 70 so it is kinda funny ) and obviously I don't NEED it though I still kinda wanna try it. 😬
Switched to Reta from sema about 3 weeks ago. Started Sema 7/11. My first venture with peptides and it was really just to try to lose "stubborn belly fat". I was mostly carnivore for over a year but hit plateaus for 6-8 months and grew frustrated with the stall. Have dropped 20lbs ( CW 179lbs ) or so since July so I'm satisfied, mostly, with this. Pinning 2mg reta ( .5 to start and then Img for a week and am at 2mg now-starting yesterday and then Thursday and intend on 3mg Mon/Thur next week. Unless I have side-effects people talk about.
So that's why I asked. Tonight I will go straight from the fridge.
Thanks for all the thoughts.
Anyway I was recommended for the tesa because I initially thought trt might explain a couple issues ( one of them lousy sleep ) however blood work came back at 860 so she couldn't prescribe test. She laughed a little when she said it ( I'm coming up fast on 70 so it is kinda funny ) and obviously I don't NEED it though I still kinda wanna try it. 😬
Switched to Reta from sema about 3 weeks ago. Started Sema 7/11. My first venture with peptides and it was really just to try to lose "stubborn belly fat". I was mostly carnivore for over a year but hit plateaus for 6-8 months and grew frustrated with the stall. Have dropped 20lbs ( CW 179lbs ) or so since July so I'm satisfied, mostly, with this. Pinning 2mg reta ( .5 to start and then Img for a week and am at 2mg now-starting yesterday and then Thursday and intend on 3mg Mon/Thur next week. Unless I have side-effects people talk about.
So that's why I asked. Tonight I will go straight from the fridge.
Thanks for all the thoughts.
cheaperseeker
GLP-1 Apprentice
Could the pa have meant, let it sit for 20 mins after reconstituting, to make sure it's fully dissolved?
Brownmar
Recently Joined
2 main reasons for letting a peptide bottle come to room temp: the peptide might have precipitated (solidify in tiny amounts) and allowing it to come to room temp allows it to re-dissolve; any condensation that could lead to contamination (on the syringe) can be wiped off.
Mr. Blonde
GLP-1 Enthusiast
The best way, after you've researched and have a good understanding, is to just do it. I take mine out of the refrigerator and immediately grip it and rip it yo
No. It was understood that it had been reconstituted and had been refrigerated and was cold... and knew that it had occurred with 2nd vial as well.
As I had said I would yesterday I took it straight from the fridge-no issues.
Just weird that it only happens on a new vial. I have one left ( from ReviveRx ) of the three that I started Tesa with. I'm using a different sellers vials at the moment ( and haven't had the "first pin" problem ).
I have another scheduled telecall Friday.
I would expect that it would be every pin and not just the first one if your premise was true. They may have thought that that had occurred. My experience eliminates it as a possibility. And he also suggested maybe take in the mornings. I decided to try it at night again just to see if it would duplicate the sleeplessness but nope, just that first shot. I did let every one of 'em sit out for 20-30 minutes for weeks but after last night I think I'm good for straight from the fridge. I'll continue that unless there's a problem that arises.
I do suspect that last ReviveRX 12mg vial will give me problems on the first pin. 😬
desinr-gal
GLP-1 Enthusiast
This is really interesting. Try turning and rolling the vial around before drawing the first time.
It sounds like a compounding pharmacy product, and they may add a substance that sinks or floats, which you draw up more of the first time, depending on how you are doing that. This would explain why it affects you just the first time. What else is listed in their ingredient list?
Heck if I know what else is listed...at the moment, anyway. Wait. I'll get vial...
12mg Tesamorelin acetate, 60mg Trehalose, benzyl alcohol ( preservative )
That answer anything for you?
desinr-gal
GLP-1 Enthusiast
From Google AI: Trehalose stabilizes peptide compounds by forming a protective glassy matrix around them, a process known as vitrification, which prevents denaturation and aggregation caused by stress like desiccation, freezing, or heat. It replaces water molecules with hydrogen bonds, maintaining the peptide's correct folded structure and acting as a chemical chaperone to preserve activity and prevent damage..
Trehalose is broken down into glucose by the enzyme trehalase in the small intestine. In individuals with a hereditary or acquired trehalase deficiency—which is common in populations like the Inuit of Greenland—the unabsorbed sugar ferments in the gut. This can lead to gastrointestinal distress, including vomiting, abdominal pain, flatulence, and diarrhea.
was your experience?
No issues like THAT AT ALL. For the last 50 years though I HAVE HAD those except the vomiting-daily. Started overseas in the Navy. They couldn't figure it out. Kaiser couldn't figure it out. SON OF A...! Extreme nausea and best described simply as forcefully urinating out of my ass. Would last for 3-10 days. SO SICK..the nausea was crippling. THought I had some worm in there reeking havoc. If I wasn't on the commode it didn't matter. I could not stop it. Tossed many pairs of dungarees over the side of the ship.
THIS STUFF...the digestion slowing, constipation, isn't a side-effect. It's a POSITIVE! lmao
