Do You Filter Your Peptides Before Use?

[keangkong]

Bacteriostatic is not the same as bactericidal.

Bacteriostatic water will have a hard time killing bacteria. It only contains .9% benzoyl alcohol. That's quite weak. Its purpose is to be strong enough to stop bacterial reproduction while being weak enough that it doesn't cause problems when inside your body. It doesn't work perfectly even in stopping bacterial from reproducing.

 

[keangkong]

Bacteriostatic is not the same as bactericidal.

70% isopropyl rubbing alcohol is bactericidal. That's why you apply it on the outside of the vial. I do know that Janoshik believes that filtering is generally unnecessary.

 

[cgall88]

I just fish my syringe out of the toothbrush cup where I store it between uses, give it a quick rinse to ready it for the next peptide and I'm good to go.

😭😭😭

 

[cgall88]

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

Thank you so much for this tip. Order placed for filters. Appreciate users like yourself that dig into some research to help us all. Top class contribution in my humble opinion. Could I please ask what in your opinion a good fridge life for reconstituted Retatrutide is, ie is 3 months too long

 

[pavlovs]

Thank you so much for this tip. Order placed for filters. Appreciate users like yourself that dig into some research to help us all. Top class contribution in my humble opinion. Could I please ask what in your opinion a good fridge life for reconstituted Retatrutide is, ie is 3 months too long

Twenty-eight days is the general guidance. I go up to six-weeks, I've read others do three months and longer. My opinion is that these meds are cheap, so why take chances? You really have to decide what your own risk tolerance is.

 

[IshimaruKenta]

Never have filtered, and never will. If there's evidence stating that these vials all dangerous and must be filtered, I'll do it. But there isn't any evidence stating otherwise. Extra careful, sure, but ain't nobody's got time for that.

 

[desinr-gal]

70% isopropyl rubbing alcohol is bactericidal. That's why you apply it on the outside of the vial. I do know that Janoshik believes that filtering is generally unnecessary.

Did he?? I dont remember that. I do remember him saying that 10-15% of products tested fail. for one reason or another. There have been many reports of various crap getting into the peptides, And others have said he filters before testing the batches...

 

[basedpepbae]

Did he?? I dont remember that. I do remember him saying that 10-15% of products tested fail. for one reason or another. There have been many reports of various crap getting into the peptides, And others have said he filters before testing the batches...

Filtering in the case of physical particles being in the peps is already worth it to me. Seen too many specs and chunks of “cardboard” in vials not too.

And yes I believe he does filter for all his tests, I remember it being so that it doesn’t damage the machinery. Not sure of his actual stance on filtering though.

 

[Nathanologist]

Never have filtered, and never will. If there's evidence stating that these vials all dangerous and must be filtered, I'll do it. But there isn't any evidence stating otherwise. Extra careful, sure, but ain't nobody's got time for that.

Costs… $1? Takes 5 min max? Cheap insurance. Maybe it just illusion of control bias. Idk.

 

[Nathanologist]

Did he?? I dont remember that. I do remember him saying that 10-15% of products tested fail. for one reason or another. There have been many reports of various crap getting into the peptides, And others have said he filters before testing the batches...

Why would he filter before testing? That completely defeats the purpose of purpose of the testing. I have never in my hours of research on janoshik of in my correspondence with the labs heard that stated or implied.

 

[trojanpeptide]

After reading this, I agree, it's wiser to filter sterilize. I just ordered a bunch of 4mm 0.22 micron PES filters and some sterile 5mL vials. I will recon in BAC and then filter to a new vial. Might even be a good way to split 30mg vials so that the second vial will have less traffic when you get to using it. Thanks for the suggestion.

 

[zpped]

Never have filtered, and never will. If there's evidence stating that these vials all dangerous and must be filtered, I'll do it. But there isn't any evidence stating otherwise. Extra careful, sure, but ain't nobody's got time for that.

What evidence do you need? Plenty of vials are contaminated in one way or another. Wether that's dangerous is a personal decision.

Why would he filter before testing? That completely defeats the purpose of purpose of the testing. I have never in my hours of research on janoshik of in my correspondence with the labs heard that stated or implied.

You must have a flawed understanding of what testing accomplishes. He absolutely filters before every mass/purity test to protect his machine.

 

[IshimaruKenta]

Costs… $1? Takes 5 min max? Cheap insurance. Maybe it just illusion of control bias. Idk.

I get that, it's just for me, I'm not seeing the benefit right now. Also, I have a 10 month old baby that I take care of, and she doesn't always let me have 5 minutes needed to recon something and then put it in a pen. 😆 I try to do it as quickly as possible because she needs attention (imagine that! lol). So adding 5 minutes to the process is a bit much for me right now. I don't discount the idea of filtering as a whole, nor do I feel anyone is wrong in doing so.

If I'm seeing enough evidence where I need to, I'll find some time. I'm 100% not like the other guy. 😏

 

[Nathanologist]

What evidence do you need? Plenty of vials are contaminated in one way or another. Wether that's dangerous is a personal decision.



You must have a flawed understanding of what testing accomplishes. He absolutely filters before every mass/purity test to protect his machine.

If you filter before a sterility test, what’s the point? I do not have a flawed under of testing, maybe for GCMS, LCMS, HPLC etc. but this discussion is about sterility- or maybe I have a flawed understanding of this discussion.

 

[pavlovs]

The evidence that there is stuff in our vials that shouldn't be in our vials is that Jano filters his peptides before testing so that the stuff in vials that isn't supposed to be in vials doesn't hurt his machines.

 

[keangkong]

Janoshik wrote: "terility generally is not an issue with lyophilizates. There are not too many microbes that can survive either lyophilization or months while lyophilized. Those that can are not quite likely to find their way into the vials." That's from https://chat.peppys.org/t/lab-faq-guide-with-janoshik-analytical/4136. Click on the .pdf document and you can read the whole thing if you belong to Peppys.