All right, i searched for freezing tirz, specifically the Jano response but...

[Gr33dyOctopus]

Didn't really see it. But know it exists. Apologize in advance if im posting questions that have been answered, just trying to get a handle on all of this.

Ok, my question is, freezing reconstituted tirz. I have this 30ml of bac water and it would be cool to use the whole thing on ten vials. Calm Logic, or Indolent, or someone else thats been helping me along here mentioned freezing bottles of tirz 10 at a time.

That maybe it was Jano that said we can freeze this shit as long as there isnt to many thaw cycles, that even after... a year or two reconstituted tirz is frozen, when thawed there is barely a poentcy loss. Is this true for others? Does anyone have a link to Jano talking about this subject?

As a bonus question has anyone tried KPV? Thoughts on it?

Thanks in advance fuckers!

 

[Sasquatch]

And why not leave the shit in powder form until it's time to pin?

 

[Grogu]

I previously searched and searched for scientific research on freezing reconstituted peptides, and specifically glp-1 medications, and I haven't been able to find anything specific to freezing glp-1s.

I did find one journal article about glp-1 and glucagon levels in frozen human plasma subjected to multiple freeze-thaw cycles. The authors found, to their surprise, little degradation after 3 freeze/thaws. Here is the article:

Stability of glucagon-like peptide 1 and glucagon in human plasma - PMC

To investigate the stability of glucagon-like peptide 1 (GLP-1) and glucagon in plasma under short- and long-term storage conditions. Pooled human plasma (n=20), to which a dipeptidyl peptidase 4 (DPP4) inhibitor and aprotinin were added, was spiked ...

pmc.ncbi.nlm.nih.gov

I think that this is the article that most people refer to when saying it's okay to freeze tirzepatide. But to me, glp-1 and glucagon levels in human plasma is fundamentally different that reconstituted lyophilized peptides in bac water.

I'm really not sure why there would be a reason to freeze reconstituted lyophilized peptides, I would think it would be better to leave those vials in the freezer until you're ready to reconstitute.

 

[Calm Logic]

It seems crazy but the more I looked into it, the more it seemed fine. I don't have any citations/sources though, just secondhand stuff about what Jano said.

People do it without any obvious issues, and something like tirz is not fragile compared to HGH or something like that. HGH is so fragile that it can easily foam if you don't use a vent needle during recon.

One reason for some is convenience:

My current routine is to reconstitute the whole kit at once, then freeze all [big pharma advises not to freeze because of the mechanisms in their autoinjectors], keeping active-use vial in fridge (or even freezer, with quick thaw before each dosing). Then freezing the leftover bac for future use. Your comfort level might vary, and a downside to this is if you get into more elaborate protocols in the future (e.g. a smaller dose, but every 5 days) so carefully label or otherwise keep track of details like actual mg and bac volume in the vials.

I know there are theoretical concerns. But we can die on the the way to the mailbox.

 

[swimmer]

As a bonus question has anyone tried KPV? Thoughts on it?

Thanks in advance fuckers!

I like it as an antiflamitory its good at knocking back the aches and pains. For me its comparable to ibprofin. It is also somewhat effective at relieving migraine headaches.

 

[Calm Logic]

I would also look into ARA 290:

Feature	KPV (Lysine-Proline-Valine)	ARA 290 (Cibinetide)
Mechanism of Action	Inhibits the intracellular NF-κB inflammatory signaling pathway.	Selectively activates the innate repair receptor (IRR) on the cell surface to promote tissue repair and block inflammation.
Efficacy in Trials	Demonstrated efficacy primarily in animal/lab models for inflammation reduction and wound healing.	Proven efficacy in human Phase II clinical trials for reducing neuropathic pain symptoms and promoting nerve fiber regeneration.
Regulatory Status	Investigational (research-grade); no human safety/efficacy data to recommend clinical use.	Investigational drug in human trials (Phase II/III completed/ongoing); not FDA-approved for general use but has Orphan Drug Status for specific conditions.
Administration	Varied in research: topical, oral (in specific formulations), or injections.	Subcutaneous injection (typically 4 mg daily in trials).

Potential Applications in Specific Pain Conditions

Condition	KPV Potential	ARA 290 Potential
Back Pain	Theoretical benefit due to general anti-inflammatory properties, but no specific studies exist.	Potential for neuropathic low back pain if nerve damage is involved; studies are ongoing regarding adjunctive treatments, but direct ARA 290 studies for general back pain are limited.
Knee Pain (Osteoarthritis)	General anti-inflammatory properties might help reduce joint inflammation, but no specific research or clinical data available.	Less specific focus in research; mechanism focuses more on nerve and organ repair than large joint mechanics, but the innate repair receptor activation could have protective benefits for joint tissues.
Sciatica	No specific research. Sciatica involves nerve root inflammation/compression.	High potential. Preclinical studies in rats with sciatic nerve injury showed long-lasting relief from pain symptoms (allodynia) and suppression of spinal cord inflammation.
Carpal Tunnel	No specific research.	High potential. The mechanism promotes nerve regeneration and reduced neuropathic symptoms demonstrated in human trials for small fiber neuropathy, which is directly relevant to nerve compression syndromes like carpal tunnel.

 

[byefatlicia]

But we can die on the the way to the mailbox

I was more likely to die on the way to the mailbox before finding glp1s 📫

 

[Habibibi]

PTDS has data on thawing reconstituted tirzepatide multiple times, and it seems like there was no loss in potency. So I would readily consider freezing reconstituted tirzepatide for later use safe.

 

[Calm Logic]

I was more likely to die on the way to the mailbox before finding glp1s 📫

Yeah, rather than freeze a reconned kit, I would rather recon a vial of T100 or R100 and split that into multiple vials for refrigeration.

My compounded, unopened, refrigerated tirz vials from Brello Health that expired months ago are still working well.

 

[byefatlicia]

I don't remember all the science, but we all tossed this around a few times. My take aways are, and this is my approach, to purchase smaller dosed kits that you can use up a vial within 30-45 days. And leave the vials in the freezer until recon. Recon what i'm gonna start using.

 

[Gr33dyOctopus]

And why not leave the shit in powder form until it's time to pin?

Honestly, it's cause I'm hip deep in all of this and just want to fuck around, learn all I can, and shoot myself up like a lab rat. Ive got soooo many mg's of different peps now and growing. And still have southend, prorx and bpi, like a lot. So, just for fun?!?

 

[Gr33dyOctopus]

I previously searched and searched for scientific research on freezing reconstituted peptides, and specifically glp-1 medications, and I haven't been able to find anything specific to freezing glp-1s.

I did find one journal article about glp-1 and glucagon levels in frozen human plasma subjected to multiple freeze-thaw cycles. The authors found, to their surprise, little degradation after 3 freeze/thaws. Here is the article:

Stability of glucagon-like peptide 1 and glucagon in human plasma - PMC

To investigate the stability of glucagon-like peptide 1 (GLP-1) and glucagon in plasma under short- and long-term storage conditions. Pooled human plasma (n=20), to which a dipeptidyl peptidase 4 (DPP4) inhibitor and aprotinin were added, was spiked ...

pmc.ncbi.nlm.nih.gov

I think that this is the article that most people refer to when saying it's okay to freeze tirzepatide. But to me, glp-1 and glucagon levels in human plasma is fundamentally different that reconstituted lyophilized peptides in bac water.

I'm really not sure why there would be a reason to freeze reconstituted lyophilized peptides, I would think it would be better to leave those vials in the freezer until you're ready to reconstitute.

Appreciate your thoughtful response!

 

[Calm Logic]

PTDS has data on thawing reconstituted tirzepatide multiple times, and it seems like there was no loss in potency. So I would readily consider freezing reconstituted tirzepatide for later use safe.

Yes, I heard about that. Forgot where that came from until you mentioned it.

 

[Gr33dyOctopus]

It seems crazy but the more I looked into it, the more it seemed fine. I don't have any citations/sources though, just secondhand stuff about what Jano said.

People do it without any obvious issues, and something like tirz is not fragile compared to HGH or something like that. HGH is so fragile that it can easily foam if you don't use a vent needle during recon.

One reason for some is convenience:



I know there are theoretical concerns. But we can die on the the way to the mailbox.

Im following you now motherfuck. You are awesome, and so is indolent, going to follow that fucker to, and probably Nathan, and airborne, and others. Its nice getting my bearings in here and seeing the people that really make a difference for me. Ty much! Yea, if I can freeze 10 vials and just use up the 30ml bac. Its not just me this is for, there's a ton of other rats in my life wanting this cheese.
Make life wayyyy easier.

 

[Gr33dyOctopus]

I like it as an antiflamitory its good at knocking back the aches and pains. For me its comparable to ibprofin. It is also somewhat effective at relieving migraine headaches.

Ty! Its on my order list!!! Thats encouraging to hear!

 

[Gr33dyOctopus]

I would also look into ARA 290:

A

I would also look into ARA 290:

That DOES look promising its on my short list ty!

 

[Gr33dyOctopus]

Yes, I heard about that. Forgot where that came from until you mentioned it.

Im gonna go for it. Trying my first reconstituted gray tomorrow, will take and freeze another 5 or even 9 and mess with them every so often to see if myself or other rats notice a decline in potency.
Yeaaaaaa boiiii!

 

[Gr33dyOctopus]

I don't remember all the science, but we all tossed this around a few times. My take aways are, and this is my approach, to purchase smaller dosed kits that you can use up a vial within 30-45 days. And leave the vials in the freezer until recon. Recon what i'm gonna start using.

Not for me, I want to buy 120mg vials just to mess around with them for kicks ang giggles 🫠

 

[Gr33dyOctopus]

PTDS has data on thawing reconstituted tirzepatide multiple times, and it seems like there was no loss in potency. So I would readily consider freezing reconstituted tirzepatide for later use safe.

Ty Habibibi

 

[byefatlicia]

Here's a thread with a discussion that influenced my thought process

Survo - Can I freeze after reconstitution?

Okay, so I'm getting close to starting my Survo research. Problem is, It's a 10mg kit and my starting dose 500mcg will take too long to use safely. My question is: would it be okay to freeze after reconstitution between administration? will that help it last the duration?

glp1forum.com

 

[Gr33dyOctopus]

Here's a thread with a discussion that influenced my thought process

Survo - Can I freeze after reconstitution?

Okay, so I'm getting close to starting my Survo research. Problem is, It's a 10mg kit and my starting dose 500mcg will take too long to use safely. My question is: would it be okay to freeze after reconstitution between administration? will that help it last the duration?

glp1forum.com

Ima read it now tanks Fatlicia!!!

 

[Calm Logic]

I think more people don't freeze because the reward doesn't seem enough for the uncertainty. OTOH, as @indolent has mentioned before, freezing is more sterile than refrigeration, which is the best argument for freezing reconned vials:

I'd be more worried about sterility issues when splitting it up into multiple vials.

I'd either move the reconnned vial in and out of freezer (it thaws quickly) or just ignore the 4-week rule and go with 12 weeks refrigerated (as I and many others here do)...assuming always alcohol-swabbing the top before puncture, including at recon.

That DOES look promising its on my short list ty!

Relatively few vendors sell ARA 290. J5 has the kits and BioBombshell has the singles from the same vendor (ZYH, which does retail but mostly bulk sales).

 

[Gr33dyOctopus]

Here's a thread with a discussion that influenced my thought process

Survo - Can I freeze after reconstitution?

Okay, so I'm getting close to starting my Survo research. Problem is, It's a 10mg kit and my starting dose 500mcg will take too long to use safely. My question is: would it be okay to freeze after reconstitution between administration? will that help it last the duration?

glp1forum.com

Read it, still not convinced not to freeze but some good info in that thread!!!

 

[Calm Logic]

Have you seen what Google Gemini says? Basically, you could be injecting Frankenstein GLP, haha. The testing doesn't confirm that, but there could be a touch of freaky in the vial.

 

[byefatlicia]

Have you seen what Google Gemini says? Basically, you could be injecting Frankenstein GLP, haha. But the testing doesn't confirm that.

kind of gives you the idea that it was paid to say that🤔

 

[Calm Logic]

But that is also the common-sense concern, that freezing is a form of destruction.

So if you have even a minuscule amount of former tirz, can it mess things up over time, if you keep injecting more freaky stuff week after week?

But the cagri controversy about fibrils seemed overblown too.

 

[Nathanologist]

Im following you now motherfuck. You are awesome, and so is indolent, going to follow that fucker to, and probably Nathan, and airborne, and others. Its nice getting my bearings in here and seeing the people that really make a difference for me. Ty much! Yea, if I can freeze 10 vials and just use up the 30ml bac. Its not just me this is for, there's a ton of other rats in my life wanting this cheese.
Make life wayyyy easier.

Have you been drinking? 🤣🤣🤣😇

 

[Nathanologist]

Im gonna go for it. Trying my first reconstituted gray tomorrow, will take and freeze another 5 or even 9 and mess with them every so often to see if myself or other rats notice a decline in potency.
Yeaaaaaa boiiii!

I’m doing it. Recon a R60 into 3x R20 vials and freeze 2. If it affects the product I figure I’ll feel it

 

[Calm Logic]

Another potential survivor! And many bros on the Meso forum inject foamy, denatured HGH every day of the year without issue.

 

[byefatlicia]

But that is also the common-sense concern, that freezing is a form of destruction.

So if you have even a minuscule amount of former tirz, can it mess things up over time, if you keep injecting more freaky stuff week after week?

But the cagri controversy about fibrils seemed overblown too.

If you ask Gemini about freezing Bac, it's not good too