...and so it begins. The BAC is cookin' in the Petri dishes

[fetefille]

I finally got around to mixing up the water for injection (WFI) and benzyl alcohol a couple of days ago. I've got several swabs on two Petri dishes going on the make-shift-y-est of make shift warmers--the tight top of the back of the fridge. It keeps a surprisingly constant warm temp.

I mentioned in another post, this ain't science science over here.

Interestingly, it smells exactly like Hospira, neither the WFI nor the BA smelled like that on their own.

I guess we'll see.

 

[Foggy-Hollow]

Top/back of fridge works well for sourdough bread! Sounds like you’ll grow some things. 🙂

 

[fetefille]

So, a little update: there was one spot small spot that started to develop in Pd #1, but didn't grow. Nothing in Pd #2. I'm chalking up to some user error/cross contamination bc it's been a min since this lab lady worked in a sperm bank lab.

I started 2 more, so far so good, not a fuzz or growth in site with naked eye. Have to be checked under a scope. Any ideas on reagents that may make that part easier? I have a feeling sperm washin' isn't the same as this. You know, I'm going to look for a centrifuge on eBay hell, even amazon. I've never had to price out my ELISA machines on down. Just put it on the "I need it and bam, there it was, lol)

I mixed feteille BAC water with clean bottle of T30, filtered, and the applied and even injected some under the "skin" of the of the PD. We'll see.

In total, I've got 6 dishes going with only 1 showing tiny spherical growth. It hasn't enlarged or died off. The last 2 are mixed with T30.

And so we (maybe just me) wait.

 

[ThatNextGril]

I am so here for the results on this experiment! 🍿🤓

 

[Foggy-Hollow]

Science geek here, still keeping an eye on your experiment. 🤍🤓

Thanks for the update!

 

[fetefille]

Ok...No growth (w/naked eye & using every alternate light source I could shine across the surface) in any Pd except for Pd #1.

The Pds 5 & 6, that were a mix of T30 and Fetefille's BAC also showed clean.

I waited a few more days, mixed a vial of T10, filtered, put on a dish. Waited as loooooong as I could, like 3-5 days (I'm a bad scientist, think more Bella Lugosi than Mr. Science). I inj 20u and waited.

No isr, no skin necrosis. no croakin'.

So just now, I tried 40u. I squirted a little on my hand as I'm a very, very olfactory-based person (actually all my senses are heightened, sometimes that's really good.) Smelled like it should. I'd watered down the T10 ridiculously so as not to mess with my regular inj, but I wanted to try something more than just more than the water.

I'd never give this to anyone, but I feel comfortable using it myself. I think I'll keep checking it once a month, maybe twice, for growth. Petri dishes aren't that expensive, maybe a few more geeky lab things.

(I'll let you know if my belly falls off) (I'm not kidding, lol) (ok, being flip, but serious)