Bacteriostatic Solution Sodium Chloride

[Jaylee]

Has anyone used the Pfizer Injection Bacteriostatic Solution Sodium Chloride (Hospira) Green top, to reconstitute any of their peptides?

 

[bbrewer]

Has anyone used the Pfizer Injection Bacteriostatic Solution Sodium Chloride (Hospira) Green top, to reconstitute any of their peptides?

There is some concern that the salt can interfere with the peptide function by changing how it folds. It would probably take a lot of digging to sort it out.

 

[scarywood75]

There is some concern that the salt can interfere with the peptide function by changing how it folds. It would probably take a lot of digging to sort it out.

It indeed cannot be used on all peptide, like BAC isn't always appropriate for pH reasons, but saline BAC is better for the most part

It makes the injection more comfortable

 

[Calm Logic]

The ingredients in Zepbound:

Active ingredient: tirzepatide. Inactive ingredients: sodium chloride, sodium phosphate dibasic heptahydrate, and water for injection.

For Wegovy:

Active Ingredient: semaglutide

Inactive Ingredients: disodium phosphate dihydrate, 1.42 mg; sodium chloride, 8.25 mg; water for injection; and hydrochloric acid or sodium hydroxide may be added to adjust pH.

According to Google Gemini:

In the context of tirzepatide reconstitution, the 0.9% sodium chloride in bacteriostatic water helps to create a physiologically compatible solution for injection while the bacteriostatic agent ensures the sterility of multi-dose vials. The sodium chloride itself is not expected to have a direct therapeutic effect or significantly alter the activity of tirzepatide when used as a diluent according to the product guidelines.

 

[TooBigtoFail]

Nacl is to make the solution more isotonic. From the COCAINE patent: It is further desirable to match the tonicity (i.e., osmolality) of body fluids at the injection site as closely as possible when administering the compositions because solutions that are not approximately isotonic with body fluids can produce a painful stinging sensation when administered. It is desirable that the compositions be approximately isotonic with body fluids at the sites of injection. The present composition comprising tirzepatide, NaCl, and dibasic sodium phosphate is associated acceptable patient injection site experience. Likewise, the composition comprising tirzepatide, proplylene glycol, and dibasic sodium phosphate is associated with acceptable patient injection site experience. .....

 

[Loves2eat]

I used it for Thymalin since the vendor I ordered from said it was required. I later found out that using regular BAC to reconstitute Thymalin is fine.

 

[esdsafepoet]

I've been thinking about getting a box of saline bacteriostatic water. It's a lot cheaper. Given what I'm reading in this thread, I'm not sure why more people aren't talking about it.

 

[Calm Logic]

Someone reported cloudiness when mixed with reta. But I have serious doubts about the saline being the reason. Even though I got a C in college chemistry, I don't see how saline BAC would be fine with COCAINE but not reta.

 

[esdsafepoet]

Someone reported cloudiness when mixed with reta. But I have serious doubts about the saline being the reason. Even though I got a C in college chemistry, I don't see how saline BAC would be fine with COCAINE but not reta.

Does reta have sodium chloride as an inactive ingredient like you mentioned above with COCAINE and sema?

Would definitely make sense that anything that already has sodium chloride in it should be fine with saline bac.

 

[Calm Logic]

My AIs can't find the inactive ingredients listed anywhere, including the clinical trials. I also couldn't find anything doing a Google site search of FDA.gov.

 

[scarywood75]

Someone reported cloudiness when mixed with reta. But I have serious doubts about the saline being the reason. Even though I got a C in college chemistry, I don't see how saline BAC would be fine with COCAINE but not reta.

I've seen the same thing on Discord and I can confirm it's bullshit, been doing that for like 8 + months and even after 2months in the fridge it's still fully transparent

 

[janedoe]

Saw this on an API/Chemical under lab description

Retatrutide (sodium salt) is supplied as a solid. A stock solution may be made by dissolving the retatrutide (sodium salt) in the solvent of choice, which should be purged with an inert gas. Retatrutide (sodium salt) is sparingly soluble (1-10 mg/ml) in DMSO and slightly soluble in acetonitrile.Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous
solutions of retatrutide (sodium salt) can be prepared by directly dissolving the solid in aqueous buffers. Retatrutide (sodium salt) in sparingly soluble (1-10 mg/ml) in PBS (pH 7.2). We do not recommend storing the aqueous solution for more than one day

 

[janedoe]

Saw this on an API/Chemical under lab description

Retatrutide (sodium salt) is supplied as a solid. A stock solution may be made by dissolving the retatrutide (sodium salt) in the solvent of choice, which should be purged with an inert gas. Retatrutide (sodium salt) is sparingly soluble (1-10 mg/ml) in DMSO and slightly soluble in acetonitrile.Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous
solutions of retatrutide (sodium salt) can be prepared by directly dissolving the solid in aqueous buffers. Retatrutide (sodium salt) in sparingly soluble (1-10 mg/ml) in PBS (pH 7.2). We do not recommend storing the aqueous solution for more than one day

The last sentence leads me to think since no bacteriostatic agent was mentioned, storage would be no more than a day....

Not fully clear on that......