Broken Chef
Senior Member
In my quest to filter peptides for my RS that has COPD and an autoimmune disease, I bought some supplies.
I am not sure how vials are rated/sized or what parameters they should have.
1. I noticed these 5ml vials are considerably larger than what peptides come in - what everyone calls 3ml vials.
Based on the fact that the peptide vials hold 3ml to the top, and these 5ml vials hold 8ml to the top, I'd venture to say that what everyone calls 3ml peptide vials, are actually 2ml vials. It would make sense that they would hold more than they are rated for.
2. These vials came from www.medlabsupply.com
They were $30 for a 25 pack. They also had other brands of vials at $40 and $50 per 25 pack. I opted for the cheaper ones, possibly a mistake as they are 18 months old with 18 months out exp date.
They arrived without negative pressure - not vacuum sealed. When I tried to create negative pressure in them, I could only get about 1ml worth before air sucked in past the needle in the rubber stopper. Some of the peptide vials have 3ml worth of vacuum and it does not suck in air past the needle when reconstituting - I am able to recon and remove air, leaving it vacuum sealed after recon.
I haven't the foggiest idea what any of this "means". It could be my OCD nitpicking things that don't matter, or these could be junk vials.
Until I order different brands, and play with them, I don't have much to compare to.
I know there are members here with decades of experience in the medical industry, so I'm wondering what you might have to say about this.
In hind sight, I should have sprung for the premium brand vials at $20 more. I let my frugality override my need to be as safe/sterile as possible.
3. Filters...
I ordered some of the Cobetter .22 micron 13mm PES syringe filters from Amazon, and also ordered some of the .22 microm 4mm PES filters from PeptideTest.
Package of the 4mm from peptide test say "Not for medical use" ... Hmmm...
PeptideTest has several YT videos playing with these filters, testing how many ml they can filter before they fail. Some interesting videos...
I also ordered 3ml and 5ml Excel syringes, and some 25g 1.5" Excel needles from medLabSupplies to reach the $75 free shipping. I already have some 22g 1" needles, but I find the needles to be "thick" and they punch a good sized hole in the rubber stoppers. The stoppers are self healing, but I figure - the smaller the hole, the less damage it does to the stopper, so I opted for slightly smaller gauge needles to use for recon and filtering.
Again, this might not be something I should even worry about. Just a few side tunnels I happened upon, while crawling through the filtering rabbit hole.
I just think that, to strive for perfection in the sterility rabbit hole, these things seem like they could hamper that. Allowing non sterile air into the vial I so diligently filtered, poses some risk, but I have no idea how much.
Perhaps my best efforts are for not and I'd be better served by not using bac past 28 days, and using up the reconstituted peptides within 4-6 weeks? I'm starting to wonder if it's worth the effort I'm putting in.
After all, endotoxins and viruses are so small, they will pass right through the .22 micron filters, so the things that could cause the most harm, are not being mitigated in this process...
I almost feel like I need a Clean Room to recon and filter in... Prob just my OCD, but wanted to throw it out there.
There seems to be growing concern, and with the massive influx of new people talking about this, let's work out the tiny details and see what's worth considering, and what doesn't really matter.
I am not sure how vials are rated/sized or what parameters they should have.
1. I noticed these 5ml vials are considerably larger than what peptides come in - what everyone calls 3ml vials.
Based on the fact that the peptide vials hold 3ml to the top, and these 5ml vials hold 8ml to the top, I'd venture to say that what everyone calls 3ml peptide vials, are actually 2ml vials. It would make sense that they would hold more than they are rated for.
2. These vials came from www.medlabsupply.com
They were $30 for a 25 pack. They also had other brands of vials at $40 and $50 per 25 pack. I opted for the cheaper ones, possibly a mistake as they are 18 months old with 18 months out exp date.
They arrived without negative pressure - not vacuum sealed. When I tried to create negative pressure in them, I could only get about 1ml worth before air sucked in past the needle in the rubber stopper. Some of the peptide vials have 3ml worth of vacuum and it does not suck in air past the needle when reconstituting - I am able to recon and remove air, leaving it vacuum sealed after recon.
I haven't the foggiest idea what any of this "means". It could be my OCD nitpicking things that don't matter, or these could be junk vials.
Until I order different brands, and play with them, I don't have much to compare to.
I know there are members here with decades of experience in the medical industry, so I'm wondering what you might have to say about this.
In hind sight, I should have sprung for the premium brand vials at $20 more. I let my frugality override my need to be as safe/sterile as possible.
3. Filters...
I ordered some of the Cobetter .22 micron 13mm PES syringe filters from Amazon, and also ordered some of the .22 microm 4mm PES filters from PeptideTest.
Package of the 4mm from peptide test say "Not for medical use" ... Hmmm...
PeptideTest has several YT videos playing with these filters, testing how many ml they can filter before they fail. Some interesting videos...
I also ordered 3ml and 5ml Excel syringes, and some 25g 1.5" Excel needles from medLabSupplies to reach the $75 free shipping. I already have some 22g 1" needles, but I find the needles to be "thick" and they punch a good sized hole in the rubber stoppers. The stoppers are self healing, but I figure - the smaller the hole, the less damage it does to the stopper, so I opted for slightly smaller gauge needles to use for recon and filtering.
Again, this might not be something I should even worry about. Just a few side tunnels I happened upon, while crawling through the filtering rabbit hole.
I just think that, to strive for perfection in the sterility rabbit hole, these things seem like they could hamper that. Allowing non sterile air into the vial I so diligently filtered, poses some risk, but I have no idea how much.
Perhaps my best efforts are for not and I'd be better served by not using bac past 28 days, and using up the reconstituted peptides within 4-6 weeks? I'm starting to wonder if it's worth the effort I'm putting in.
After all, endotoxins and viruses are so small, they will pass right through the .22 micron filters, so the things that could cause the most harm, are not being mitigated in this process...
I almost feel like I need a Clean Room to recon and filter in... Prob just my OCD, but wanted to throw it out there.
There seems to be growing concern, and with the massive influx of new people talking about this, let's work out the tiny details and see what's worth considering, and what doesn't really matter.