Filter yes or no

[Memo85]

Fairly new to the game. Couple months. Have received good test scores with failed sterility on a couple batches. I will filter everything going forward. Cheap insurance.

 

[eidos]

I also plan on bringing back my microbiology lab days and DIY sterility testing on agar plates

I considered that too, but it’s a bit complicated because it requires two or three culture media, and anaerobic bacteria will be missing. I saw that there are protocols in the pharmacopoeia, but it’s even more complicated in a mini-lab.

Have you thought of a specific protocol?

 

[Chili777]

More reasons to filter below. Per the FDA (when it was the FDA).

 

[NorthStarNomad]

I am extremely new to peptides. In fact, today was my first day. 😅

After spending months of time (if not more) reading research papers, forum discussions, watching videos, and safety information, I decided to use a filter. I completely understand that there is always some level of risk involved, especially when sourcing research peptides, but I wanted to add every reasonable safety measure I could.

For me, it is less about assuming there is a problem and more about having an extra layer of protection and peace of mind. The filters are relatively inexpensive, so if there is even a small chance they help remove particulates or reduce risk, I feel the cost is well worth it.

At the end of the day, I know it is still a gamble to some extent, but I would rather take advantage of every safety measure available than skip one and wish I had not. 🤷‍♀️😅

 

[HouseCat]

I considered that too, but it’s a bit complicated because it requires two or three culture media, and anaerobic bacteria will be missing. I saw that there are protocols in the pharmacopoeia, but it’s even more complicated in a mini-lab.

Have you thought of a specific protocol?

I haven’t gone that deep yet, but I got a good idea for a liquid culture medium from here, and I’m thinking if I could put it in a vial and somehow maintain the vacuum partially, or even just filling it to the cap/self sealing lid, that would be neat for anaerobic testing, and agar and blood agar are pretty cheap and easy to get. I also have a test tube warmer that may come into play. It’s been 10 years since I was in microbiology and I loved it, but a lot of that knowledge was pushed to the side from years of grad school, if you buy into Sherlock’s brain attic idea 😂

 

[eidos]

It's a great idea to do it in a vial in a liquid medium. It will probably require a spectrophotometer.

My microbiology certificate and my Droso counter badge are probably older than you ;-) But the aGLP-1s have helped me regain the cognitive acuity I had in my 20s. It’s easy and enjoyable again.

I find "mental palace" cooler.
😎

 

[chewonmysac]

ah ok thanks for clarifying that for me. I just started tesa last night.... spicy af 😂

It will wear your belly out with lumps and irritation. I switched to Glutes after a week and never looked back.

 

[chewonmysac]

I have the whole filter set up, but have not used it. I bought it to have precautionary measures in place. My risk threshold is higher than most. If something does not feel or look right, I toss the vial, which I have only done a couple of times. Never a whole kit. Some singles. I am much more conscious now that I am gray, and I use the vials more quickly because I control the timeline. I will probably never use my Tele Health Tirz stash, as some are approaching 8-12 months and now understand the degradation process despite the preservatives 503a,b use. That was a pretty expensive lesson.

 

[Sasquatch]

Does everyone understand filtering originated because of glass ampules?
Have a look at the size of the molecules you wish to filter.
Have a look at the filter sizes available.
How clean is the room or booth you are filtering in?

 

[Grogu]

I will probably never use my Tele Health Tirz stash, as some are approaching 8-12 months and now understand the degradation process despite the preservatives 503a,b use. That was a pretty expensive lesson.

I used them and even past BUD and never had an issue. This past week I pinned with some Hallendale that was really kinda old. I'm almost out of compound, but I'm using it.

 

[chewonmysac]

I used them and even past BUD and never had an issue. This past week I pinned with some Hallendale that was really kinda old. I'm almost out of compound, but I'm using it.

It is funny. My BPI no additive did awesome after it expired, but my Olympia faded quickly.

 

[Grogu]

It is funny. My BPI no additive did awesome after it expired, but my Olympia faded quickly.

I loved BPI. I was sad when I used my last vial. End of an era. I lost a lot of weight using BPI. The OG of the compounded scene.

 

[px1984]

In terms of the vials you are putting the filtered peps back into, are people sterilising them there selves or assuming that purchased vials are sterile?

 

[Rezwalker]

a simple no would sufice ...

PEP ROULETTE FTW

 

[GLiPtest]

Just my 2 cents — yes, filtering will add a few extra bucks and save you a lot of headache. That said, I think it's worth adding a small disclaimer here. I'm not saying you're one of these people, but I've heard a lot of 'I filter because I don't want to deal with endotoxins' and... those filters won't do a thing against endotoxins. They'll slide right through.

But bacteria won’t. Endotoxin is far less of a threat than the cumulative effect of hundreds of injections of other contaminants, bacteria, aggregates, and inorganics like glass and plastics.

Or just trust your health to the humanitarians we all know the criminals operating unlicensed Chinese underground labs are.

 

[Sasquatch]

I used them and even past BUD and never had an issue. This past week I pinned with some Hallendale that was really kinda old. I'm almost out of compound, but I'm using it.

How was the Hallendale? I can't even remember the strength? I think was 5 or 10mg. It was "screwing in a canoe." (Fucking near water)

 

[Grogu]

How was the Hallendale? I can't even remember the strength? I think was 5 or 10mg. It was "screwing in a canoe." (Fucking near water)

Not my favorite, but I took 20mg this past Saturday and it felt feels good this week, but I know a lot of folks who think it's really week. The concentration is so low I had to pin 135 units 🤷🏻‍♂️.

 

[bay_area_schizo]

It will wear your belly out with lumps and irritation. I switched to Glutes after a week and never looked back.

I'm sort of an ass man myself 🤣 might make the switch also

 

[Rosco P. Coltrane]

I just started filtering. I just assume the vials that I buy are sterile. Be careful if your buying from Amazon.

 

[ThatNextGril]

Recon. Draw. Remove needle, attach filter, attach new needle. Inject into a clean, depyrogenated, sterile vial. Doesn't hurt to also attach a vent.
If using a 13mm filter, flush with 0.2ml bac to push the held back peptide solution into the vial.

This was the most direct, easy to follow instruction on filtering I've seen so far. Ty ty!

 

[BNLFL]

In terms of the vials you are putting the filtered peps back into, are people sterilising them there selves or assuming that purchased vials are sterile?

My say sterile when purchased. No clue if the are, I haven't had any issues.

 

[mtomm]

Granted I'm super new, but I don't see a downside to filtering since it costs $1-3 a filter and doesn't take more than a few minutes.

Plus, I love gear, including lab supplies. I like to feel as much like a mad scientist as possible!

This is my feelings exactly!

 

[desinr-gal]

So you would reconstitute, draw with filter and put into a new sterile vial

search filtering filters etc on here there are very detailed instructions and discussions.
Also videos on You tube.. peptidetest and others. It's easier to understand when you watch the process.
There have been accounts of people finding bits of unknown stuff floating in their peptides besides the danger of bacteria, which some deny. I'd much rather be safe than sorry. and you do get to feel like a scientist.. 😁
many vendors of supplies sell kits for this process. Including Peptidetest.com, underground supply, injection shop..

 

[TazaTaza]

Passing it through a 0.22 filter does not make it sterile lol...just bacteria free? I don't know why that is a prevailing thought in this community.

It doesn't make any sense to me. If ur worried about filtering out bacteria there is a bigger problem at hand a filter won't save you from an unsafe vial. Just toss it if u visibly see something floating in ur recon'd vial

If there is bacteria and other contaminants in ur vial you will still contract some type of reaction bc the filter won't catch endos or viruses, misfolded peps, contamination from synthesis etc.

In the lab we use filters bc we are ASSUMING what we are handling is sterile, and it's just a failsafe to remove bacteria bc it's easy to contaminate whatever ur handling w/ur hands or even breath. We don't think it's not sterile from the jump. Think of filtering as a minimizing contamination technique, it's not a decontamination technique.