Filtering

[iamiPAC]

And a list, like the one above, is more helpful when showing how relatively good vendors go bad or disappear, as in an exit scam.

Please don’t mind the New Researcher Question: What is a Filtering Kit? Why would you need to filter your peptides? Please expand my mind with wisdom. 🤔 LOL..

 

[Calm Logic]

Actually, I love this question, even though it has been debated several times. Basically, it comes down to your risk tolerance. Most people don't filter. I do, as do some others here.

For one thing, most people and vendors don't test for sterility.

Many will argue that subq injections will face the body's own defenses, which is true. But, as @hexagonal once pointed out, even subclinical infections can increase the risk of arthritis down the line:

Do You Filter Your Peptides Before Use?

You are a fat clown, here to attention seek rather to provide information. Here's a study showing that about 25% of vials get contaminated on repeated access: https://journals.lww.com/jehp/fulltext/2018/07000/A_pilot_study_for_evaluation_of_knowledge_and.120.aspx Now you are calling people...

glp1forum.com

Also, the recon process itself can create particulates that would ideally be filtered out. And when things are tested for purity by Jano, they filter first, so that their machine doesn't get messed up. Vials from vendors have contained small amounts of foreign material, which can harbor bacteria.

A primary argument against filtering is that the filtering process itself can introduce bacteria from one's home. But wearing gloves and wiping things down with alcohol can go a long way. So I don't really buy that argument, especially since, emotionally, international bacteria from Shanghai seems scarier than bacteria from my home.

Having said all that, I don't think filtering is usually necessary, unless one is immunocompromised. And other aspects of recon are just as important, such as using other sterile techniques, like wearing gloves to prevent staph infection.

An example of a filtering kit:

Reconstitution and Filtering all-in-one Starter Kit

NOTE - Clear 3ml Vials are on backorder - Amber 3ml Vials will be substituted.Our Reconstitution and Filtering Starter Kit provides everything you need to safely reconstitute and filter water-soluble peptides. This kit includes all the components demonstrated in our instructional videos...

peptidetest.com

Similar items can be purchased individually at Peptide Test, Amino Annex, AllThingsPeptides, or in a kit as well at Underground Supply (on vacay until the 1st).

 

[GLP1Pharmacist]

While test peptides are likely not made to full FDA standards in the real compounding pharmacies filtered needles are almost never used. Only practical application is when small all glass (no stopper or cap) ampules are actually broken open, the filter needle prevents risk of glass particles alone. As soon as drawn up the filter needle is discarded and new needle attached. I have zero plans to ever use a filter needle for glp1s. Plus filter needles will not filter out things which could actually cause problems like chemicals and pyrogens.

 

[Peotidethrowaway]

I do filter most the time. Sometimes I won’t filter if it’s just a one off I’m trying. When I started bacteria contamination and filtering was all the rage so that’s just how I learned, I guess. Plus I’m using a pen cart and it’s literally zero extra steps anyway since I’m transferring it to the new vial I just bac flush and attach the filter.

At some point I do agree that the more you futz with your meds you’re just introducing contamination vectors but whatever

The sterility of the pen carts is a whole other matter.

I don’t wear gloves and I don’t wipe the kitchen down. I do put down a super sterile paper towel to work on though. I’m still alive.

 

[Calm Logic]

The crazy thing is that even doing recon and injections in a dirty bathroom would be safer than getting an injection from some med spas.

 

[Peotidethrowaway]

The crazy thing is that even doing recon and injections in a dirty bathroom would be safer than getting an injection from some med spas.

Valid.

 

[iamiPAC]

Actually, I love this question, even though it has been debated several times. Basically, it comes down to your risk tolerance. Most people don't filter. I do, as do some others here.

For one thing, most people and vendors don't test for sterility.

Many will argue that subq injections will face the body's own defenses, which is true. But, as @hexagonal once pointed out, even subclinical infections can increase the risk of arthritis down the line:

Do You Filter Your Peptides Before Use?

You are a fat clown, here to attention seek rather to provide information. Here's a study showing that about 25% of vials get contaminated on repeated access: https://journals.lww.com/jehp/fulltext/2018/07000/A_pilot_study_for_evaluation_of_knowledge_and.120.aspx Now you are calling people...

glp1forum.com

Also, the recon process itself can create particulates that would ideally be filtered out. And when things are tested for purity by Jano, they filter first, so that their machine doesn't get messed up. Vials from vendors have contained small amounts of foreign material, which can harbor bacteria.

A primary argument against filtering is that the filtering process itself can introduce bacteria from one's home. But wearing gloves and wiping things down with alcohol can go a long way. So I don't really buy that argument, especially since, emotionally, international bacteria from Shanghai seems scarier than bacteria from my home.

Having said all that, I don't think filtering is usually necessary, unless one is immunocompromised. And other aspects of recon are just as important, such as using other sterile techniques, like wearing gloves to prevent staph infection.

An example of a filtering kit:

Reconstitution and Filtering all-in-one Starter Kit

NOTE - Clear 3ml Vials are on backorder - Amber 3ml Vials will be substituted.Our Reconstitution and Filtering Starter Kit provides everything you need to safely reconstitute and filter water-soluble peptides. This kit includes all the components demonstrated in our instructional videos...

peptidetest.com

Similar items can be purchased individually at Peptide Test, Amino Annex, AllThingsPeptides, or in a kit as well at Underground Supply (on vacay until the 1st).

THANKS so much for that detailed explanation and truly calm logic. I love it. 😍 You really know your stuff.

There is a lot to learn and I'm excited to learn all about the in's ans out's of peptide preparation and filtering. I will have to get one of those kits when it’s back in stock. Do you filter each and every vial?

 

[iamiPAC]

While test peptides are likely not made to full FDA standards in the real compounding pharmacies filtered needles are almost never used. Only practical application is when small all glass (no stopper or cap) ampules are actually broken open, the filter needle prevents risk of glass particles alone. As soon as drawn up the filter needle is discarded and new needle attached. I have zero plans to ever use a filter needle for glp1s. Plus filter needles will not filter out things which could actually cause problems like chemicals and pyrogens.

That is a good point. I can’t believe ampules are still a thing. Thanks for your comment.

 

[iamiPAC]

I do filter most the time. Sometimes I won’t filter if it’s just a one off I’m trying. When I started bacteria contamination and filtering was all the rage so that’s just how I learned, I guess. Plus I’m using a pen cart and it’s literally zero extra steps anyway since I’m transferring it to the new vial I just bac flush and attach the filter.

At some point I do agree that the more you futz with your meds you’re just introducing contamination vectors but whatever

The sterility of the pen carts is a whole other matter.

I don’t wear gloves and I don’t wipe the kitchen down. I do put down a super sterile paper towel to work on though. I’m still alive.

THANKS for the comment. Another good point. I just added some sterile paper sheets to my Amazon cart to use next time.

 

[Calm Logic]

Do you filter each and every vial?

Yes. I am very much on the OCD spectrum though.

 

[yobculture]

I don’t wear gloves and I don’t wipe the kitchen down. I do put down a super sterile paper towel to work on though. I’m still alive.

😂 Same!

I’m going to put “Super Sterile Paper Towel” on a t-shirt!

 

[Peotidethrowaway]

THANKS for the comment. Another good point. I just added some sterile paper sheets to my Amazon cart to use next time.

I was being sarcastic about the paper towels being sterile. I use them right off the roll. Probably cross contaminated with chicken juice

 

[iamiPAC]

Yes. I am very much on the OCD spectrum though.

I work in healthcare so I’m for sure on the spectrum. LOL.. 😂 If I can find a system and an affordable way to do this I will. I am currently researching 7 different peptides and prepare my syringes on Sundays, so… 🤔

 

[pavlovs]

I filter almost everything, including already compounded vials such as lipo-c. The only thing I don't filter is glutathione, and that is because it is a brand that supplies hospitals and is made on the up and up. I use a sterilized tray and wear gloves and a mask.

 

[yobculture]

I filter almost everything, including already compounded vials such as lipo-c. The only thing I don't filter is glutathione, and that is because it is a brand that supplies hospitals and is made on the up and up. I use a sterilized tray and wear gloves and a mask.

Do you put a filter on the vent needle? I really don’t understand why this would be necessary since most people push air into their reconstituted vials to help with extraction when pinning. I don’t put a filter on the vent needle, but I’d love to hear an explanation from a more experienced (and smarter) person on why they do use a filter on the vent needle.

 

[iamiPAC]

I filter almost everything, including already compounded vials such as lipo-c. The only thing I don't filter is glutathione, and that is because it is a brand that supplies hospitals and is made on the up and up. I use a sterilized tray and wear gloves and a mask.

What kit do you use? I wouldn't mind starting if I could get an affordable and reliable source for the kits.

 

[pavlovs]

What kit do you use? I wouldn't mind starting if I could get an affordable and reliable source for the kits.

If you mean the glutathione, I use a Korean company that sells items for use in cosmetic procedures, like Botox, etc. I use the brand Luthione, it's made by a proper pharmacy. The only three companies I have bought from are Acecosm.com, meamoshop.com and derma-solution.com/ There are a lot of others though, but I have never used them. Shipping is less than a week for all of them. It's around $95.00 for 1200mg X10 vials.

If you mean the Lipo-C, I use SNP.

 

[pavlovs]

Do you put a filter on the vent needle? I really don’t understand why this would be necessary since most people push air into their reconstituted vials to help with extraction when pinning. I don’t put a filter on the vent needle, but I’d love to hear an explanation from a more experienced (and smarter) person on why they do use a filter on the vent needle.

I am definitely not that experienced, I've only been using peptides since last November. I'm also not that smart 🙂 I pull from the vial without a vent. Then I remove the just-used needle from the syringe, add the vent, and replace the needle onto the vent with a new needle.

 

[AndyPanda]

If you mean the glutathione, I use a Korean company that sells items for use in cosmetic procedures, like Botox, etc. I use the brand Luthione, it's made by a proper pharmacy. The only three companies I have bought from are Acecosm.com, meamoshop.com and derma-solution.com/ There are a lot of others though, but I have never used them. Shipping is less than a week for all of them. It's around $95.00 for 1200mg X10 vials.

If you mean the Lipo-C, I use SNP.

My most recent order of Glutathione from Acecosm wasn’t delivered after two weeks and that company is less help than Chinese UGL vendors. lol 0 out of 5 stars.

 

[yobculture]

I am definitely not that experienced, I've only been using peptides since last November. I'm also not that smart 🙂 I pull from the vial without a vent. Then I remove the just-used needle from the syringe, add the vent, and replace the needle onto the vent with a new needle.

Actually, that’s a relief! That’s all I do, too. I was probably just impressed because you wore a mask and gloves. I don’t wear a mask, but sometimes I wear gloves. It’s hard getting the pull-apart filter packaging opened while wearing gloves, so it just annoys me. If I don’t wear gloves, I just spray my hands thoroughly with my bottle of alcohol.

 

[pavlovs]

My most recent order of Glutathione from Acecosm wasn’t delivered after two weeks and that company is less help than Chinese UGL vendors. lol 0 out of 5 stars.

Yeah, makes sense. That may be true of the other Korean companies as well. There's a lot of stockpiling going on by Americans because of tariff issues and items less than $800.00 no longer being exempt. Also, like peptides, there's been an increased focus and spotlight on the do-it-yourself cosmetic procedure crowd. Probably off of the Korean companies are as overwhelmed with orders. Btw, I didn't get glutathione from Acecosm, they don't sell my preferred brand (or at least they didn't used to), the other two sell Luthione.

 

[yobculture]

What kit do you use? I wouldn't mind starting if I could get an affordable and reliable source for the kits.

Several people have told you where to get kits, including me in the post below. I also mention that you can source supplies for your own kit by looking at the supplies listed in the Filtering Kit (CLICK LINK). I started with the kit because I liked having a visual of exactly what I needed. After that, I was able to get my own filtering supplies based off that kit. That will save a lot of money.

I don’t have any wisdom to share, but I think filtering is a good idea. I didn’t want to do it because it looked too fussy, but it was a lot easier than I thought it would be. The Filtering Kit that a lot of people buy is presently sold out, but you can recreate the supplies by looking at their list and purchasing separately. The instructional video here is too long and fussy, but it will give you a good idea of the process. I still don’t understand why one would need to put a filter on the vent needle, since you’re probably going to inject air into the vial later when you dose, but whatever. They probably just want to sell more filters. 🤷🏻‍♂️

I personally like this video the best, save for the filter on the venting needle. (I don’t put a filter on the venting needle. Not medical advice.)

 

[nonyabizznez]

Actually, that’s a relief! That’s all I do, too. I was probably just impressed because you wore a mask and gloves. I don’t wear a mask, but sometimes I wear gloves. It’s hard getting the pull-apart filter packaging opened while wearing gloves, so it just annoys me. If I don’t wear gloves, I just spray my hands thoroughly with my bottle of alcohol.

I don't wear gloves when I recon/filter and I don't filter the vent needle. I'm no authority but it's my personal comfort level. I've yet to see any real benefit from filtering a vent except maybe for the people selling filters.

 

[desinr-gal]

Several people have told you where to get kits, including me in the post below. I also mention that you can source supplies for your own kit by looking at the supplies listed in the Filtering Kit (CLICK LINK). I started with the kit because I liked having a visual of exactly what I needed. After that, I was able to get my own filtering supplies based off that kit. That will save a lot of money.

I had these same questions.. I went to Ali express to get all the stuff and then found out they won't ship anything to me. arrg. I guessed the vent filter is because there's a vacuum in the vial so it sucks in air. If your space isnt super sterile that air could contaminate?? I was wondering about that as I live in a very humid non sterile place. I have another question too, How much volume of product can one filter filter? I saw other posts where a larger filter than was used in the video is recommended. not the 4mm.
Will filtering save me from having to ship out to test?

 

[pavlovs]

https://peptidetest.com/collections/lab-supplies

The site that yobculture posted is a website where you can order a filter kit, enough for several vials. I bought my first kit from this site, they are legit. Now I buy just what I need from other sources. You can also buy individual pieces if you'd rather.

The possibility of air contaminating the vials is real, this is why some folks may choose not to filter, and others choose to filter with an air purifier present.

The larger filters lose a little more of the solution than the small filters. Either should be fine for peptides. I use one filter per 1 syringe of solution, regardless of the amount of solution in that syringe.

Filtering doesn't replace a test. Filtering doesn't tell you the purity or quantity (mgs) that is in the vial. Filtering can remove bacterial contaminates, or particles that don't need to be there, like flies and seamonkeys 🙂

If I got any of this wrong, I am sure someone will tell me 🙂 Good luck!

 

[yobculture]

The possibility of air contaminating the vials is real, this is why some folks may choose not to filter, and others choose to filter with an air purifier present.

I guess this is the part that really confuses me. It seems that many, if not most, people inject air into their reconstituted vial each time before extracting their peptide. Are they contaminating the contents of their vial each time they do that?

 

[iamiPAC]

https://peptidetest.com/collections/lab-supplies

The site that yobculture posted is a website where you can order a filter kit, enough for several vials. I bought my first kit from this site, they are legit. Now I buy just what I need from other sources. You can also buy individual pieces if you'd rather.

The possibility of air contaminating the vials is real, this is why some folks may choose not to filter, and others choose to filter with an air purifier present.

The larger filters lose a little more of the solution than the small filters. Either should be fine for peptides. I use one filter per 1 syringe of solution, regardless of the amount of solution in that syringe.

Filtering doesn't replace a test. Filtering doesn't tell you the purity or quantity (mgs) that is in the vial. Filtering can remove bacterial contaminates, or particles that don't need to be there, like flies and seamonkeys 🙂

If I got any of this wrong, I am sure someone will tell me 🙂 Good luck!

NOT THE SEAMONKEYS! 🐵 LOL.. 😂

 

[pavlovs]

I am not a scientist by any means, but I do read a lot. I think that a generally healthy person may not have to worry too much about the air, as long as the environment is relatively clean. Filtering the solution shouldn't add anymore air than we add from a non filtered vial, or at least add no more air than those who pull air
into the syringe and then shoot the room air into the vial to equalize the pressure.

I don't know that as a fact, though. I'm just hypothesizing that one is no worse than the other.

I guess this is the part that really confuses me. It seems that many, if not most, people inject air into their reconstituted vial each time before extracting their peptide. Are they contaminating the contents of their vial each time they do that?

 

[Calm Logic]

There's also other reasons to filter, such as to avoid subclinical inflammation from particulates formed during recon.

 

[Calm Logic]

That's the first type mentioned here:

Particulates can come from a wide variety of sources, including:

• The drug substance itself: Yes, if a peptide (or any drug compound) doesn't fully dissolve, or if it aggregates or precipitates out of solution (due to changes in pH, temperature, concentration, or interaction with other components), it can form particulates. Peptides are particularly prone to aggregation and insolubility, especially longer or more hydrophobic ones.
  
  
• Excipients: Other ingredients in a formulation might not fully dissolve.
  
  
• Container/Packaging: Small pieces of glass, rubber (from stoppers), plastic, or metal can shed from vials, syringes, or other packaging materials during manufacturing, storage, or handling.
  
  
• Manufacturing Equipment: Wear and tear on equipment can introduce metal, plastic, or other debris.
  
  
• Environmental Contaminants: Dust, fibers (from clothing, wipes), skin flakes, or other airborne particles can enter a product if manufacturing environments are not sufficiently controlled (e.g., cleanrooms).
  
  
• Chemical Reactions: Unexpected chemical reactions within the product over time can lead to the formation of insoluble byproducts.
  
  
• Microbial Contamination: While not solid in the same way, microbial growth (bacteria, fungi) can also be considered a particulate contaminant.