In Novo’s Own Words: Degradation of Amylin Analogs Such as Cagrilintide (and How to Test For It)

[hexagonal]

In theory it should help prevent additional fibril (and oligomer? maybe?) formation though, so it wouldn't be the worst idea.

I am not an expert and this whole subject made me cancel my prior plan to order some cagri to stack with my reta, but if I was going to go through with it, this is what I would do:

1) Use smaller dosage vials so I go through them more quickly
2) Reconstitute and get the pH to 4.0
3) Filter after to remove as many of the larger clumped fibrils as I can

From some posts in the other forums, it looks like NN is now testing some single chamber pens with combined CagriSema, though we don't yet seem to have details on the pH, buffering agent used, etc.

 

[peptideusername]

oligomer?

 

[exploitedworkerbee]

View attachment 4210

This will never be played out. Please never ever ever stop.

 

[JohnnyGobbs]

Anyone have a recommendation for ph test strips you guys use for testing ph, preferably something on Amazon. Thx

 

[zpped]

Anyone have a recommendation for ph test strips you guys use for testing ph, preferably something on Amazon. Thx

It's hard to go wrong with ph paper. It's an extremely basic thing all over amazon.

 

[kussmaul]

To summarize it from that thread, it is obvious the centrifuge test was meant to be a proxy for potential long term degradation - that was the whole point. A 45 hour test is not useful for any sort of commercial applications otherwise, since no one prescribed cagrilintide would be taking it within 45 hours of formulation, or using it in a centrifuge at high heat. It is an attempt to recreate the sort of results they expect cagrilintide might have when reconstituted against the long term. And, ultimately, they could have gone with that formulation at a pH of 7.5 and not had to develop a whole new pen that kept cagrilintide and semaglutide separate, significantly increasing their cost, but instead decided that doing so was necessary.

Not that it really matters to these argument, but the testing referenced was not done using a centrifuge. A fluorescence plate reader was used. The plate reader moved the plate in a 1 mm orbit 960 rpm, so small fast circles. Centrifuge speeds are much faster with more movement. Centrifuges are meant to separate whatever is being spun into components, the plate reader is meant to keep the solution mixed (usually keep the fluorescence from settling).

 

[49metal]

extremely basic

 

[YoYoFat]

It's hard to go wrong with ph paper. It's an extremely basic thing all over amazon.

Sincere question from a lay person.

I think I understand that a big question surrounds pH and stability of pH in its relation to the very bad things that might form and turn your pancreas and brain into mush.

And I get that the pep might have a pH of X upon reconstitution, but doesn’t the pH of the stuff you inject into your body change to Y upon mixing with “body stuff”?

 

[hexagonal]

Not that it really matters to these argument, but the testing referenced was not done using a centrifuge. A fluorescence plate reader was used. The plate reader moved the plate in a 1 mm orbit 960 rpm, so small fast circles. Centrifuge speeds are much faster with more movement. Centrifuges are meant to separate whatever is being spun into components, the plate reader is meant to keep the solution mixed (usually keep the fluorescence from settling).

Clarification is always good. Someone said centrifuge at some point and I just started copying it.

I think it does matter, though, since it sounds like this is significantly less of a torture test than it has been made out to be.

 

[zpped]

And I get that the pep might have a pH of X upon reconstitution, but doesn’t the pH of the stuff you inject into your body change to Y upon mixing with “body stuff”?

I'm not commenting on cagrilintide, but checking the ph of any pep can save you some pain. But yes your body will change the ph of any fluid you put in it.

 

[JohnnyGobbs]

 

[Super Trips]

I wonder if cagri is more or less risky than those mail order bath salts I got into for a spell during grad school. If that stuff didn’t get me I can’t believe this stuff will.

hey those bath salts were fun.... but did you die? well not yet at least