SS-31 protocols?

[Chuck11]

've been trying SS-31 for a clinically diagnosed mitochondrial disorder, linked to heterozygous PINK1 and OPA1 mutations. I've been taking large doses—15 to 30 mg per day for a week. It's hard to say if it's having a positive effect so far—maybe it would have been more effective if I'd started 15 years ago when the condition became full-blown. The goal is to upregulate mitochondrial fusion (defragmentation) via the working OPA1 copy.

I noticed that clinical trials use massive doses. Does anyone know why SS-31 is so expensive or if there's a way to get it cheaper? I probably need to buy a gram.

At this point, I'm planning to either get somewhat healthier or die trying—no joke. After a decade of promises, a Harvard-trained biomedical geneticist and mitochondrial expert told me there’s nothing in the drug pipeline—nothing panned out—so self-experimentation is my only option.

 

[Vicki]

've been trying SS-31 for a clinically diagnosed mitochondrial disorder, linked to heterozygous PINK1 and OPA1 mutations. I've been taking large doses—15 to 30 mg per day for a week. It's hard to say if it's having a positive effect so far—maybe it would have been more effective if I'd started 15 years ago when the condition became full-blown. The goal is to upregulate mitochondrial fusion (defragmentation) via the working OPA1 copy.

I noticed that clinical trials use massive doses. Does anyone know why SS-31 is so expensive or if there's a way to get it cheaper? I probably need to buy a gram.

At this point, I'm planning to either get somewhat healthier or die trying—no joke. After a decade of promises, a Harvard-trained biomedical geneticist and mitochondrial expert told me there’s nothing in the drug pipeline—nothing panned out—so self-experimentation is my only option.

The best price I've seen was qsc has it on sale for 260 during black Friday and I see it in a group buy now for same price but that's not even going into production until after chiense new year. Both those were 50mg sizes

 

[GimmeABreak]

've been trying SS-31 for a clinically diagnosed mitochondrial disorder, linked to heterozygous PINK1 and OPA1 mutations. I've been taking large doses—15 to 30 mg per day for a week. It's hard to say if it's having a positive effect so far—maybe it would have been more effective if I'd started 15 years ago when the condition became full-blown. The goal is to upregulate mitochondrial fusion (defragmentation) via the working OPA1 copy.

I noticed that clinical trials use massive doses. Does anyone know why SS-31 is so expensive or if there's a way to get it cheaper? I probably need to buy a gram.

At this point, I'm planning to either get somewhat healthier or die trying—no joke. After a decade of promises, a Harvard-trained biomedical geneticist and mitochondrial expert told me there’s nothing in the drug pipeline—nothing panned out—so self-experimentation is my only option.

You're right. If I am remembering correctly the phase 2 and phase 3 trials I reviewed for elamipritide (SS-31) dosed at 40mg/day for like 6-12 months. That's a payment on a nice car (for people that finance cars)!

It's actually getting ready to be an FDA approved med for Barth Syndrome in 1H-2025.

Stealth Biotherapeutics Announces FDA Acceptance of New Drug Application for Elamipretide for the Treatment of Barth Syndrome - Stealth BioTherapeutics Inc.

<div class="at-above-post addthis_tool" data-url="https://stealthbt.com/stealth-biotherapeutics-announces-fda-acceptance-of-new-drug-application-for-elamipretide-for-the-treatment-of-barth-syndrome/"></div>Barth Syndrome is an Ultra-rare, Progressive, Life-Shortening, Cardioskeletal Disease that...

stealthbt.com

 

[Chuck11]

You're right. If I am remembering correctly the phase 2 and phase 3 trials I reviewed for elamipritide (SS-31) dosed at 40mg/day for like 6-12 months. That's a payment on a nice car (for people that finance cars)!

It's actually getting ready to be an FDA approved med for Barth Syndrome in 1H-2025.

https://stealthbt.com/stealth-bioth...ide-for-the-treatment-of-barth-syndrome/[/URL

Also, the route is typically intravenous:

Participants were randomized to intravenous elamipretide (0.01, 0.1, and 0.25 mg/kg/h or placebo for 2 hours in a dose-escalating sequence). The primary efficacy measure was the change in distance walked in the 6-minute walk test (6MWT) after 5 days of treatment. https://www.neurology.org/doi/pdfdirect/10.1212/WNL.0000000000005255

With intramuscular, a good portion will be lost. I was thinking of taking it as an eyedrop. I will try 60mg in one shot next. But at $89 x 2 from Sky Peptides, this is running a pretty penny. Is that has an appreciable effect, I guess I will try to buy a gram for 2.5k https://www.selleckchem.com/products/elamipretide-mtp-131.html

 

[GimmeABreak]

The price at Sellechkchem is $2290 for a gram.

QSC's price mentioned in the previous post is $520 for a gram. Which is two 50mg kits @ $260/kit (a kit is 10 vials). The site you linked also doesn't state if they are selling raws or lyophilized and trust me, you don't want to deal with raws.

 

[GimmeABreak]

With intramuscular, a good portion will be lost. I was thinking of taking it as an eyedrop.

I normally just go SQ, the thought of putting it directly into my eye is a little to cringy for me.

 

[Valerie]

I have had great results with SS-31. Tons of energy and countered the Tirz fatigue.

I pinned 4-5mg in the AM daily for most of October and November after titrating up to to 4mg over about two weeks. Some days I would talso take a 3mg booster dose in the afternoon. I added MOTs-C for about 3-4 weeks but noticed no additional benefits. I am currently taking a week off from peps (except Tirz) but will likely start it back up again next week.

My opinion (with zero medical/scientific training to back this up) based on reading feedback on the forums is if you old and didn't live a healthy lifestyle for most of your life you will get benefit from SS-31 (I'm 58). If you are young or middle-aged and mostly lived clean, it will probably be a nothing-burger for you.

Not sure about the old AND unhealthy lifestyle.

I'm over 50 but have lived a healthy lifestyle.

Mitochondrial function simply declines as we age.

 

[Chuck11]

The price at Sellechkchem is $2290 for a gram.

QSC's price mentioned in the previous post is $520 for a gram. Which is two 50mg kits @ $260/kit (a kit is 10 vials). The site you linked also doesn't state if they are selling raws or lyophilized and trust me, you don't want to deal with raws.

Link to QSC? 1 gram is 1000mg, not 100mg, so I would need 20 50mg kits. As for ocular application, it makes a difference if you have a degenerative condition -- you become more risk-tolerant.

 

[GimmeABreak]

Link to QSC? 1 gram is 1000mg, not 100mg, so I would need 20 50mg kits. As for ocular application, it makes a difference if you have a degenerative condition -- you become more risk-tolerant.

Damn, you had me second-guessing myself. A 50mg kit has 500mg (ten 50mg vials) so two kits = 1000mg = 1g.

Look in the vendor connection forum (on this site), QSC advertises there but only order through Tracy. Search this site to learn more about them, they are not necessarily a vendor for beginners. Also, they are on hiatus probably because of the Chinese New Year or maybe because they were arrested, but reach out to them in early Feb when they are back online. I am sure they will have new promos to kick things back off.

Other CNY vendors stock SS31 but most only stock 30mg (300mg) kits.

Nexaph/SNP is US based and has a preorder right now that will ship in the next week or so, 300mg for $220 with free two-day shipping. They are in the vendor connection channel or you can find them here: https://nexaph.com/product/pre-order-ss-31-30mg-10-vials-kit/

 

[Chuck11]

Damn, you had me second-guessing myself. A 50mg kit has 500mg (ten 50mg vials) so two kits = 1000mg = 1g.

Look in the vendor connection forum (on this site), QSC advertises there but only order through Tracy. Search this site to learn more about them, they are not necessarily a vendor for beginners. Also, they are on hiatus probably because of the Chinese New Year or maybe because they were arrested, but reach out to them in early Feb when they are back online. I am sure they will have new promos to kick things back off.

Other CNY vendors stock SS31 but most only stock 30mg (300mg) kits.

Nexaph/SNP is US based and has a preorder right now that will ship in the next week or so, 300mg for $220 with free two-day shipping. They are in the vendor connection channel or you can find them here: https://nexaph.com/product/pre-order-ss-31-30mg-10-vials-kit/

Thank you so much for the advice! I'll look into it and keep you all updated. This should serve as a canary-in-the-coal-mine test case, as in theory, SS31 upregulates fusion via OPA1.

 

[GimmeABreak]

Thank you so much for the advice! I'll look into it and keep you all updated. This should serve as a canary-in-the-coal-mine test case, as in theory, SS31 upregulates fusion via OPA1.

Since you have a legitimate medical diagnosis have you tried getting a Doc to write a prescription to a compound pharmacy and seeing if your health insurance would cover it?

Please keep us posted on your process to create a eye drop and your progress. I would imagine you would want to reconstitute with sterile water or saline? This means the vials become one-time-use as there is no alcohol to prevent bacterial growth. I don't think BAC water in your eye would be very enjoyable.

 

[Chuck11]

Today I tried 60mg from Skype Peptides. The effect wasn't huge but it wasn't nothing. Increased mental clarity. My dystonia was more controlled. I had tried AC peptide before and it did nothing -- probably junk. Will order the 50mg x 10 set and try 100mg for 5 days.
.

 

[Chuck11]

Since you have a legitimate medical diagnosis have you tried getting a Doc to write a prescription to a compound pharmacy and seeing if your health insurance would cover it?

Please keep us posted on your process to create a eye drop and your progress. I would imagine you would want to reconstitute with sterile water or saline? This means the vials become one-time-use as there is no alcohol to prevent bacterial growth. I don't think BAC water in your eye would be very enjoyable.

I see Dr. Fran Kendall, an expert in the field out of Atlanta. Also specialists at Duke. Complex 1,3 defects, seemingly downstream a couple of mutations. They were pessimistic. There were high hopes for Elimipretide but they didn't plan out in the trials during the last 5 years. She said she would prescribe if it ever was approved. I doubt she would write a script for a compounder but I'll ask. I only recently discovered that you could buy as SS31. I was on rapamycin for a while -- with the help of some crazy guys from longecity - and that was a miracle drug via upregulation of mitophagy. In the last 5 years, I was able to travel, due 500 miles of the Camino de Santiago, present research at a small conference, relearn how to drive, write a book, and take care of my dad during his last two years. But homeostasis kicks in along with disease progression. At some point it's like trying to empty the ocean with a teaspoon.

 

[Chuck11]

The best price I've seen was qsc has it on sale for 260 during black Friday and I see it in a group buy now for same price but that's not even going into production until after chiense new year. Both those were 50mg sizes

Where do you see the group buy info? Also, do you guys do group testing?

 

[GimmeABreak]

Where do you see the group buy info? Also, do you guys do group testing?

If you have an immediate need, the Nexaph link I sent is probably going to be the fastest for a kit or two. Cain/Nexaph just got the latest batch in stock today, he takes CC's and ships two-day for free. I have a bunch of SS31 from Cain/Nexaph from a previous batch and I am very happy with it.

QSC or the previously mentioned group buy could be a good second buy for you. You will likely not receive it until mid-late February due to the Chinese new year. If you are going to wait for the group buy testing (assuming its included) it will likely add another month to this. Group buys are great but typically take 6-12 weeks (or longer) to complete, especially if they include 3rd party testing.

Let me know if I can help in any way.

 

[Chuck11]

Ok, thanks. Ordered and recived Nexaph; I did 50mg today in one arm shot. Will let you know if I feel anything.

 

[Chuck11]

Ok, thanks. Ordered and recived Nexaph; I did 50mg today in one arm shot. Will let you know if I feel anything.

The effect with Nexaph SS31 is much more subtle than with Skye SS31. How would I go about getting this third-party tested? I would be willing to pay to test SS31 from various companies so that we all know what we are getting. My condition is not something to monkey around with. And I am not in the mood to piss money down the drain on junk.

 

[GimmeABreak]

The effect with Nexaph SS31 is much more subtle than with Skye SS31. How would I go about getting this third-party tested? I would be willing to pay to test SS31 from various companies so that we all know what we are getting. My condition is not something to monkey around with. And I am not in the mood to piss money down the drain on junk.

The only reliable testing house that I know that can test SS31 is https://janoshik.com/. Jano is the gold standard of testing in the gray market.

You may get additional options from others on the site but I recommend you stay away from Vanguard and Forever Young Pharmacy. I would also not share the mg in the vial you are sending if you send to anyone but Jano. Cain/Nexaph normally sets up 3rd party testing through a coordinator on this Telegram channel. If they have started it yet on this batch you could volunteer to provide a vial and get your test for free. You can find the link on his website (at the bottom of the page).

 

[Fisherman2356]

If you have an immediate need, the Nexaph link I sent is probably going to be the fastest for a kit or two. Cain/Nexaph just got the latest batch in stock today, he takes CC's and ships two-day for free. I have a bunch of SS31 from Cain/Nexaph from a previous batch and I am very happy with it.

QSC or the previously mentioned group buy could be a good second buy for you. You will likely not receive it until mid-late February due to the Chinese new year. If you are going to wait for the group buy testing (assuming its included) it will likely add another month to this. Group buys are great but typically take 6-12 weeks (or longer) to complete, especially if they include 3rd party testing.

Let me know if I can help in any way.

I agree with Nexaph being a very reliable and quick to ship supplier. I've sourced 4-5 different pareptides from them and the overall experience is night and day compared to other Chinese suppliers I've used. As for quality I couldn't independently verify since the effect of many peptides is rather subtle to me and Nexaph are no different, and I have never sent vials for testing, so I guess I'll take their testing/word for it, but that seems the case for most suppliers also. As for price, it is very competitive considering they only charge $5 for shipping, and free I think on orders over $200.

Out of curiosity what is your (anybody's) perception regarding Nexaph's product quality as compared to let say other Chinese suppliers?

 

[chmuse]

I agree with Nexaph being a very reliable and quick to ship supplier. I've sourced 4-5 different pareptides from them and the overall experience is night and day compared to other Chinese suppliers I've used. As for quality I couldn't independently verify since the effect of many peptides is rather subtle to me and Nexaph are no different, and I have never sent vials for testing, so I guess I'll take their testing/word for it, but that seems the case for most suppliers also. As for price, it is very competitive considering they only charge $5 for shipping, and free I think on orders over $200.

Out of curiosity what is your (anybody's) perception regarding Nexaph's product quality as compared to let say other Chinese suppliers?

If you get the chance, look at the threads the vendor has posted in. Lots of info if you read the entire threads.

 

[Fisherman2356]

If you get the chance, look at the threads the vendor has posted in. Lots of info if you read the entire threads.

Ok thanks for the reply, I think I misspoke. my question should have been: Has any group/person ever tested Nexaph's product independently/on their own?

 

[chmuse]

Ok thanks for the reply, I think I misspoke. my question should have been: Has any group/person ever tested Nexaph's product independently/on their own?

Yes, most of his products get tested third party after the fact. There's an entire telegram for it.

 

[lazygardener]

Trial dosages are in the range of 20mg (low dose trials)-40mg/day sub-q. Based on that, there's no reason to think less than 1mg/day is going to do literally anything besides give your an occasional bruise from the injection. Here's an example for dry eye age related macular dysfunction. https://clinicaltrials.gov/study/NCT06373731?intr=Elamipretide&rank=4

This is a peptide where human clinical trial data actually exists. 500mcg is pointless.

 

[rkl123]

Trial dosages are in the range of 20mg (low dose trials)-40mg/day sub-q. Based on that, there's no reason to think less than 1mg/day is going to do literally anything besides give your an occasional bruise from the injection. Here's an example for dry eye age related macular dysfunction. https://clinicaltrials.gov/study/NCT06373731?intr=Elamipretide&rank=4

This is a peptide where human clinical trial data actually exists. 500mcg is pointless.

Placebo effect is a hell of a drug.

 

[Chuck11]

Trial dosages are in the range of 20mg (low dose trials)-40mg/day sub-q. Based on that, there's no reason to think less than 1mg/day is going to do literally anything besides give your an occasional bruise from the injection. Here's an example for dry eye age related macular dysfunction. https://clinicaltrials.gov/study/NCT06373731?intr=Elamipretide&rank=4

This is a peptide where human clinical trial data actually exists. 500mcg is pointless.

40mg/daily was also used for the mito clinical trial. https://pmc.ncbi.nlm.nih.gov/articles/PMC10382259/ Prohibitively expensive at current prices.

 

[Skidude]

After looking at many different options my first cycle was 4mg 5days on 2 days off for 6 weeks.
I am now ready for my next and will be stacking with overlaps as well- SS-31, Mots-C, NAD+
This is my current SS-31 protocol- Going with Basic first. 5mg a day also taking CoQ10,TMG,Lipo-c w/b12

Basic SS-31 Cycle (General Health & Mitochondrial Support)

Dosage: 5-10 mg subcutaneous (SQ) daily

Duration: 6-8 weeks

Break Between Cycles: 6-8 weeks off

Considerations:

Take on an empty stomach for better mitochondrial uptake.

Pair with Lipo-C, B12, or CoQ10 for enhanced mitochondrial function.

Combine with low-impact exercise (walking, bodyweight training) for best results.

Advanced SS-31 Cycle (Athletic Recovery & Performance)

Dosage: 10-20 mg SQ daily

Duration: 8-12 weeks

Break Between Cycles: Equal to cycle length (8-12 weeks)

Additional Support:

Lipo-C/B12 injections for metabolism & energy.

Creatine, TMG, and CoQ10 for added mitochondrial support.

Cold therapy or sauna use to enhance mitochondrial function.

Optional: Microdosing Approach (for Longevity & Maintenance)

Dosage: 5 mg SQ 3-4x per week

Duration: Ongoing with periodic breaks

Breaks: 2-4 weeks off every 3 months

 

[Calm Logic]

After looking at many different options my first cycle was 4mg 5days on 2 days off for 6 weeks.

Thanks for the info. More info on your first cycle:

Post in thread 'I'm going to shoot for losing 100 pounds in 6 months'

Mar 23, 2025

i'm gonna keep up with the ss31. people say it helps take weight off. one thing besides glp1 at a time.

I tried the 4mg daily, 5day on 2day off, protocol then stack mots after 4 weeks. continue for a few weeks with both. I did add the TMG. I am not big on feels, more like gradual changes, but am tempted to try to cycle up on next pass. just very costly vs other stacks. My energy was very low as a result f tirz and very low calorie I assume, it seems to have provided a boost. I have steadily dropped 1.5lbs per week over the last 7-8 weeks on cycle. But am considering the...

• Skidude

Post in thread 'Did you ever feel like you knew everyone on a TV show?'

Mar 13, 2025

that's good information thank you.

what do you think of ss31? i have a kit and i'm itching to do it.

It is tough to say, I am hoping it is helping with the mots etc.. not a huge "feel" for me, but i did notice some increased energy, which on 1200 calories is much needed. I also hope it helps with the Mots-c efficacy. I have been taking TMG supplement to help with those as well.
Overall any effects would be positive, and worth the first cycle for sure.. IMHO

• Skidude

 

[Skidude]

Thanks for the info. More info on your first cycle:

Post in thread 'I'm going to shoot for losing 100 pounds in 6 months'

Mar 23, 2025

i'm gonna keep up with the ss31. people say it helps take weight off. one thing besides glp1 at a time.

I tried the 4mg daily, 5day on 2day off, protocol then stack mots after 4 weeks. continue for a few weeks with both. I did add the TMG. I am not big on feels, more like gradual changes, but am tempted to try to cycle up on next pass. just very costly vs other stacks. My energy was very low as a result f tirz and very low calorie I assume, it seems to have provided a boost. I have steadily dropped 1.5lbs per week over the last 7-8 weeks on cycle. But am considering the...

• Skidude

Post in thread 'Did you ever feel like you knew everyone on a TV show?'

Mar 13, 2025

that's good information thank you.

what do you think of ss31? i have a kit and i'm itching to do it.

It is tough to say, I am hoping it is helping with the mots etc.. not a huge "feel" for me, but i did notice some increased energy, which on 1200 calories is much needed. I also hope it helps with the Mots-c efficacy. I have been taking TMG supplement to help with those as well.
Overall any effects would be positive, and worth the first cycle for sure.. IMHO

• Skidude

I am far from an expert on any of this, and most of the time blindly trying to find my way in the dark, but I do believe this is like many other supplements/peps/meds in that it is very specific to individual physiology.
If a person with ADHD takes Adderall it calms them down, it has a very different effect if you do not.
Same with B12 deficiency, and many other related issues.
This makes it hard to pinpoint what is working- And makes all of this anecdotal IMHO.

Elamipretide (SS-31), a synthetic tetrapeptide, is being investigated for its potential to help individuals with damaged mitochondria. SS-31 is designed to selectively target mitochondria, aiming to restore their function, particularly in conditions like mitochondrial diseases and aging.

Maybe my body needed ss-31 to help other peps work, maybe it did not.
Perhaps it is just a motivation to improve that is the real "pep" in my step.
I may never know.

For now, I am happy with the Red pill or the Blue pill, the steak is delicious, albeit much smaller than it used to be.

 

[Calm Logic]

Yeah, I'm mostly interested in the info for a relative with mild/moderate chronic kidney disease. But the ideal, more experimental dosing is very expensive. So I see it more as an add-on to the more affordable BPC-157, which may also help with CKD.

The Mitochondrion: A Promising Target for Kidney Disease - PMC

Mitochondrial dysfunction is important in the pathogenesis of various kidney diseases and the mitochondria potentially serve as therapeutic targets necessitating further investigation. Alterations in mitochondrial biogenesis, imbalance between ...

pmc.ncbi.nlm.nih.gov

Mitochondria-targeted peptide SS-31 attenuates renal injury via an antioxidant effect in diabetic nephropathy - PubMed

Oxidative stress is implicated in the pathogenesis of diabetic kidney injury. SS-31 is a mitochondria-targeted tetrapeptide that can scavenge reactive oxygen species (ROS). Here, we investigated the effect and molecular mechanism of mitochondria-targeted antioxidant peptide SS-31 on injuries in...

pubmed.ncbi.nlm.nih.gov

I think what may help the most about this research is finding cheap supplements that would help with similar pathways. Supplements such as MitoQ, CoQ10, Alpha-Lipoic Acid (ALA), N-Acetylcysteine (NAC), L-Carnitine, Resveratrol, Green Tea Polyphenols (EGCG), and Curcumin/Tumeric.

More info on SS-31 for CKD:

SS-31, a mitochondria-targeting peptide, has shown promise in ameliorating kidney disease, including Chronic Kidney Disease (CKD). Several studies suggest it can:

• Improve kidney function: By lowering urinary albumin excretion, proteinuria, serum creatinine, and BUN.
  
  
• Protect against oxidative stress: SS-31 can reduce reactive oxygen species (ROS) production, preventing damage to renal cells.
  
  
• Reduce inflammation: It can inhibit the production of pro-inflammatory cytokines.
  
  
• Prevent mitochondrial dysfunction: SS-31 protects mitochondrial structure and function, which is crucial in kidney health.
  
  
• Protect podocytes: SS-31 has shown protective effects on podocytes, cells vital for kidney function, in diabetic kidney disease models.

However, it's important to note that while preclinical studies and some clinical trials show positive results, further research is needed to confirm these findings and establish the long-term effectiveness and safety of SS-31 for CKD. Some studies also indicate that the effectiveness of SS-31 can vary depending on the specific cause and model of kidney injury.

 

[Peloma]

For now, I am happy with the Red pill or the Blue pill, the steak is delicious, albeit much smaller than it used to be.

I feel ya. I've been on 5mg daily for 11 days. Starting Mots-c on day 14. 5mg daily with two days off (3/2). Ss-31 will probably always be part of the rotation since I have felt some energy and focus gains.