sudsy peps after recon? Is this ok?

[desinr-gal]

I just reconned 3 new peps; Semax, Selank, and Mots-c. First after adding bac water the Semax was super bubbly almost effervescent.
Then after having difficulty with filtering, the filter popped and the pepetide wooshed into the vial. I repeated this twice! Now Its practically sudsy.
The Mots-C had a similar experience with the filter and its now bubbly on the surface..
Can anyone tell me if this has happened to them. Or if you would use these??
The selank procedure was smooth, no filter problem, no bubbles.
????
Is anyone else having trouble with the Tisch 4mm filters?

 

[skunknuts]

Let em settle before doing anything with them. How violent was your BAC squirt?

 

[desinr-gal]

Let em settle before doing anything with them. How violent was your BAC squirt?

it went really fast! the vials were very vacuumed.

 

[ksharpe]

Not with those particular peptides, but when reconstituting other peptides with bac stat water - sometimes I will intentionally slow the plunger down so the vacuum doesn't pull it so quickly. Helps with the bubbles.

 

[nonyabizznez]

I just reconned 3 new peps; Semax, Selank, and Mots-c. First after adding bac water the Semax was super bubbly almost effervescent.
Then after having difficulty with filtering, the filter popped and the pepetide wooshed into the vial. I repeated this twice! Now Its practically sudsy.
The Mots-C had a similar experience with the filter and its now bubbly on the surface..
Can anyone tell me if this has happened to them. Or if you would use these??
The selank procedure was smooth, no filter problem, no bubbles.
????
Is anyone else having trouble with the Tisch 4mm filters?

When discharging BAC from the syringe into the lyophilized peptide position your fingers so you can inhibit the plunger's motion through the barrel. Slowing down the saturation and angling so BAC travels down the side of the vial will inhibit foaming.

That being said let your reconned peptides sit several minutes before filtering. The luer connection needs to be secure and tight on the syringe and filter. Filter slowly and if still encountering problems you may need to use a less fine gauge needle. Obviously using a vent needle in the destination vial makes things go smoother.

 

[desinr-gal]

When discharging BAC from the syringe into the lyophilized peptide position your fingers so you can inhibit the plunger's motion through the barrel. Slowing down the saturation and angling so BAC travels down the side of the vial will inhibit foaming.

That being said let your reconned peptides sit several minutes before filtering. The luer connection needs to be secure and tight on the syringe and filter. Filter slowly and if still encountering problems you may need to use a less fine gauge needle. Obviously using a vent needle in the destination vial makes things go smoother.

I really appreciate all the tips..thank you for taking the time.
I was using a blue 23G? needle, like in the Peptesters video. Do you recommend bigger? Im worried about coring the cap. The liquid was only going in drop...........drop............drop etc. and I did add a 25G vent needle. Is that normal to be so slow?
Other times I've filtered either went much more smoothly, or became completely blocked.
I'm sure there is something I'm not catching that Im doing wrong.

 

[cookieguggleman]

When discharging BAC from the syringe into the lyophilized peptide position your fingers so you can inhibit the plunger's motion through the barrel. Slowing down the saturation and angling so BAC travels down the side of the vial will inhibit foaming.

That being said let your reconned peptides sit several minutes before filtering. The luer connection needs to be secure and tight on the syringe and filter. Filter slowly and if still encountering problems you may need to use a less fine gauge needle. Obviously using a vent needle in the destination vial makes things go smoother.

Talk to me like I"m a child....I'm about to get my first research shipment. From what I've researched, I have to take a large luer syringe, withdraw the desired amount of BAC and slowly insert into pep vial. Then swirl it a bit and let it sit. Am I missing anything? What's the filter you're referring to?

 

[Airborne Daddy]

Also make sure ur needle/filter is tight and locked.... I've had needles separate from the barrel during injection and blow shit all over.

 

[nonyabizznez]

I really appreciate all the tips..thank you for taking the time.
I was using a blue 23G? needle, like in the Peptesters video. Do you recommend bigger? Im worried about coring the cap. The liquid was only going in drop...........drop............drop etc. and I did add a 25G vent needle. Is that normal to be so slow?
Other times I've filtered either went much more smoothly, or became completely blocked.
I'm sure there is something I'm not catching that Im doing wrong.

I use 25G but only GLPs. Maybe you're peptides are more viscous from a higher concentration and further dilution is warranted (?) I'm just thinking out loud...23G seems generous. Also you may not be allowing enough time for the solution to incorporate.

 

[AndyPanda]

What kind of Bacteriostatic Water is it? Having a weird recon with different peptides at the same time seems pretty coincidental.

 

[texmexpep]

I really appreciate all the tips..thank you for taking the time.
I was using a blue 23G? needle, like in the Peptesters video. Do you recommend bigger? Im worried about coring the cap. The liquid was only going in drop...........drop............drop etc. and I did add a 25G vent needle. Is that normal to be so slow?
Other times I've filtered either went much more smoothly, or became completely blocked.
I'm sure there is something I'm not catching that Im doing wrong.

Remember you are filtering. Drip drop...it's ok...I started to love that "slow" flow....lol I had a few different peps that had more filler dissolve super slow and after filtering I was very happy to be in the "clear"

 

[desinr-gal]

What kind of Bacteriostatic Water is it? Having a weird recon with different peptides at the same time seems pretty coincidental.

Hospira, brand new bottle.

 

[desinr-gal]

I use 25G but only GLPs. Maybe you're peptides are more viscous from a higher concentration and further dilution is warranted (?) I'm just thinking out loud...23G seems generous. Also you may not be allowing enough time for the solution to incorporate.

This all makes sense.
I think I didnt wait the whole 10 mins. The more sudsy recon had extra bac, though, 3ml to 10mg.. but the Mots was only .5ml to 10mg..
I think the combo of firehose bac shooting in, lack of time to settle, forcing the filtering all led to this. It settled down more later.. Thank you.

 

[nonyabizznez]

Talk to me like I"m a child....I'm about to get my first research shipment. From what I've researched, I have to take a large luer syringe, withdraw the desired amount of BAC and slowly insert into pep vial. Then swirl it a bit and let it sit. Am I missing anything? What's the filter you're referring to?

You've got it! The majority of people recon as you've described.

Filtering is an additional step some people take. You can search "filtering" here for lively discussion on the potential merits and decide for yourself. I'm medicated low immunity (anti rejection) so I spend the .89¢ per vial on filtering. Here's a video:

View: https://youtu.be/fmz4241qPn4?feature=shared

Full disclosure I don't wear gloves or use an additional filter on the vent needle. The source of the video sells filters and for a time was the exclusive source of one manufacturer's 4mm filters. More common are 13mm filters which depending on your filtering technique can leave varying bits of peptide in the filter. Both filters leave small percentages behind but I don't do concentrated recons and overfill offsets residual peptide. Ks-tek who offers popular vials on Amazon now offers a competing 4mm filter on their website: https://ks-tek.com/