Thermos Freezer Test - Long-term storage

[GimmeABreak]

You are a heck of a person for doing this!

I wish we were going to be alive to see the results, but with all of these fibrils we're forming, even if we are alive in q4 2025, we won't be capable of understanding the results from your experiment.

At least we can leave the results for the next group of peptide researchers from 2026 who discover our fossils.

"Hey boss, come look at these fibril ridden fossils! They seem to be holding some sort of scientific papers."

I agree, not sure where I will be in Q4-25 or if I will still be obsessed with peptides so no commitment.....

I was considering NOT testing the batches on incoming and just testing when i am ready to use them but the advice I got here was it's better to know what you have. I also don't even know if Triz is my long-term GLP. I am probably going to try Reta for maintentice if my heart can handle it. If this is the case I just threw a bunch of money way on stocking up and testing.

Justification: Its only a few months of buying name brand Zep.

 

[beefsteak]

Got my Zojirushi thermos in. Threw in my yolink thermometer to test things out and it stopped temperature swings completely. I'll make a 3d printed vial holder for it over the weekend and share it here once it's done. Will spray it with shellac to prevent small plastic pieces from leaching onto the vials. It should be able to hold at least 30-40 vials stacked.

https://www.amazon.com/dp/B0BTSYQ5D4

 

[GimmeABreak]

Got my Zojirushi thermos in. Threw in my yolink thermometer to test things out and it stopped temperature swings completely. I'll make a 3d printed vial holder for it over the weekend and share it here once it's done. Will spray it with shellac to prevent small plastic pieces from leaching onto the vials. It should be able to hold at least 30-40 vials stacked.

https://www.amazon.com/dp/B0BTSYQ5D4

View attachment 3476

Looks good. Better buy a second thermos, you'll quickly fill it up.

 

[Lullabytreehugger]

Alright everyone, I realize I may not have been clear before. The numbers I shared are extrapolated from the existing knowledge we have on the topic, and they're the most accurate estimates we can provide right now.

If you’re looking for hard, factual numbers, new studies would need to be conducted, so feel free to start research and share your findings!

I didn’t mean to spark a debate. I only posted them because having an approximate estimate is better than nothing, in my opinion.

For those interested in diving deeper, here are some links to relevant resources (in no specific order):

• Peptide Stability and Potential Degradation Pathways
• New insights into the protein stabilizing effects of trehalose by comparing with sucrose
• Alternative Excipients for Protein Stabilization in Protein Therapeutics: Overcoming the Limitations of Polysorbates
• Effectiveness of Lyoprotectants in Protein Stabilization During Lyophilization
• For Lyophilization, Excipients Really Do Matter
• Lyophilization Technology for Improving Stability of Small and Large Molecules
• Mechanical Behavior and Structure of Freeze-Dried Cakes
• A great book I plan on purchasing at some point - Protein Formulation and Delivery

You are one smart cookie matey! An asset to these forums! Thank you so much!
May I ask are you a pharmacist or a microbiologist or something along these lines?

 

[Lullabytreehugger]

No my guy I was not being sarcastic.
Actually I wanted to ask after you reconstitute sema or reta. If kept in the fridge after 4 months how much % do you lose?

 

[Dee X]

No my guy I was not being sarcastic.
Actually I wanted to ask after you reconstitute sema or reta. If kept in the fridge after 4 months how much % do you lose?

The best way to know is to get it tested now and then after 4 months

 

[GimmeABreak]

The best way to know is to get it tested now and then after 4 months

I plan to do this next October with one of my freezer vials of Tirz that I just had tested. It will give me a better idea of whether I need to toss/sell my stash and start over or if I am good for another year or two. I expect (hope) to lose only a mg or two.

 

[MingHenry]

I had a public Discord discussion with a chemist who is active on Peppys and in PTDS testing server and on various Discord channels - and I've looked at the PTDS testing on frozen/fridge/room temp, and because I'm not a scientist, I could only read the graphs and sort of surmise, so when I asked him if I needed to be doing more than a manual defrost freezer, essential oil cases, EO cases in 2 baggies with desiccant packages, he said it was probably overkill. The peps don't freeze, there is no water left in them after the lyophilization process - or should not be. They are vacuum sealed - or should be, and so won't be affected by moisture - if not vacuum sealed not much can be done about that. He said keep them in the freezer as cold as possible for long term. Refrigerator for in-use. Yes, avoid freezer/room temp, freezer/room temp. Vacuum sealing not necessary, except for one's own comfort. Thermoses and 3d boxes and all of that, not necessary, my baggies that hold my EO cases, not necessary, nor are the desiccant packages. I am keeping the baggies and des. packs because why not. But after yesterday, I felt better about how I am storing the increasingly large stash of GLPs and other non-GLP peps. So much better that I ordered 4 more EO cases and this time skipped Amazon and went to Aliexpress.

 

[MingHenry]

I plan to do this next October with one of my freezer vials of Tirz that I just had tested. It will give me a better idea of whether I need to toss/sell my stash and start over or if I am good for another year or two. I expect (hope) to lose only a mg or two.

I hope you'll post that testing! Would be great to see and to know!

 

[GimmeABreak]

I had a public Discord discussion with a chemist who is active on Peppys and in PTDS testing server and on various Discord channels - and I've looked at the PTDS testing on frozen/fridge/room temp, and because I'm not a scientist, I could only read the graphs and sort of surmise, so when I asked him if I needed to be doing more than a manual defrost freezer, essential oil cases, EO cases in 2 baggies with desiccant packages, he said it was probably overkill. The peps don't freeze, there is no water left in them after the lyophilization process - or should not be. They are vacuum sealed - or should be, and so won't be affected by moisture - if not vacuum sealed not much can be done about that. He said keep them in the freezer as cold as possible for long term. Refrigerator for in-use. Yes, avoid freezer/room temp, freezer/room temp. Vacuum sealing not necessary, except for one's own comfort. Thermoses and 3d boxes and all of that, not necessary, my baggies that hold my EO cases, not necessary, nor are the desiccant packages. I am keeping the baggies and des. packs because why not. But after yesterday, I felt better about how I am storing the increasingly large stash of GLPs and other non-GLP peps. So much better that I ordered 4 more EO cases and this time skipped Amazon and went to Aliexpress.

Maybe overkill, but why not?

I have read several sources that freeze/thaw cycles degrade the peps. Obviously, the freeze/thaw cycle in a frost free freezer adds to this but the temp swing in a small freezer (even manual defrost) are pretty big, Just opening the door and adding room temp products will bring the temp well above zero. With the thermos my peps stay at exactly -8.7 degrees over a 24 hour period. The desiccant packs keep the relative humidity at 6.7-6.8%. This $35 investment is a pretty low cost insurance policy.