What is Janoshik reporting in mg

[Labcat]

I’m so confused now because I was readng last night and Peter Magic said the mass they report on the typical peptide analysis is not the net peptide content, because we don’t need it.

He also said the purity only means the percent of the desired peptide among the peptide/fragments, NOT what %of the vial contents is your peptide, how do we know how much medication is actually present? (The problem is that lyophilized peptide has the counterion, so if we weigh the puck, it’s not all med. The counterion and residual solvent is not identifiable using the hplc method he uses, he said.)

Related, I’m also now unsure whether or not fillers are in the puck, for tirz or other peptides. I have read that everyone puts it in and also that they would be identified inpurity tests, that the fillers stabilize the lyophilate and make the medication even visible, but is any of that true?

Wiser heads, what say you?

 

[Labcat]

I’m so confused now because I was readng last night and Peter Magic said the mass they report on the typical peptide analysis is not the net peptide content, because we don’t need it.

He also said the purity only means the percent of the desired peptide among the peptide/fragments, NOT what %of the vial contents is your peptide, how do we know how much medication is actually present? (The problem is that lyophilized peptide has the counterion, so if we weigh the puck, it’s not all med. The counterion and residual solvent is not identifiable using the hplc method he uses, he said.)

Related, I’m also now unsure whether or not fillers are in the puck, for tirz or other peptides. I have read that everyone puts it in and also that they would be identified inpurity tests, that the fillers stabilize the lyophilate and make the medication even visible, but is any of that true?

Wiser heads, what say you?

Partly answering my own question piece by piece lol.

Yes there must be filler in the vial bc 10mg of product would be barely visible. Knowing that, mannitol and other sugars are used to stabilize the delicate peptides while undergoing the lyophilization.

Also knowing that, I’d guess that once I reconstitute, freezing tirz again is likely to be safe enough (freeze in the coldest part of your freezer, like where the air blows from) since it had to undergo freezing already. That’s what I’m going to go with, that plus others report success. And supposedly testing has been done (I have not gotten into the PTDS group yet, but supposedly there is something there, I hear. If anyone knows of that…)

 

[FNG33]

Why recon lyophilized powder only to freeze it again? Seems counter intuitive especially if more than 1 vial at a time.

 

[Labcat]

Why recon lyophilized powder only to freeze it again? Seems counter intuitive especially if more than 1 vial at a time.

If you have extra you can’t use in time, of course.

 

[Gr33dyOctopus]

If you have extra you can’t use in time, of course.

Seriously, I want to have stuff ready to go. I have a few bottles of frozen tirz and reta that have been dated an reconstituted.

 

[Labcat]

Seriously, I want to have stuff ready to go. I have a few bottles of frozen tirz and reta that have been dated an reconstituted.

That’s a great idea also. I can see it really would be a bit of effort to set up a clean work area and do all the mixing and filtering. Not that I don’t love playing with my hoard over detailed labwork, but my cat can at any time get let into the room by my family and cause trouble.

 

[Labcat]

Seriously, I want to have stuff ready to go. I have a few bottles of frozen tirz and reta that have been dated an reconstituted.

G.O., what are you using to label?

 

[FNG33]

Just seems silly to me to freeze a bunch of vials and make the subject to temperature degradation if the power or freezer fail vs shelf stable non-temp sensitive material. I just can't wrap my head around the risks of having to trash hundreds of dollars of product over a 5 minute process, but to each his own. 🤷‍♂️

 

[Labcat]

Just seems silly to me to freeze a bunch of vials and make the subject to temperature degradation if the power or freezer fail vs shelf stable non-temp sensitive material. I just can't wrap my head around the risks of having to trash hundreds of dollars of product over a 5 minute process, but to each his own. 🤷‍♂️

Not that I’m trying to convince anyone else, but I’d actually been thinking that specific risk is likely far less than it seems at first glance. Tirz itself is stable weeks to possibly months (according to some hearsay from compounders). So if you freeze inside a thermos or thermal mass to modulate temperature swings, you probably don’t have to worry unless the power goes out for a month. And after a day you’ll probably know and have done something. And the freeze thaw seems to cause destabilization on the order of 1% (I’ve heard it in those janoshik interviews that it’s 0.2% for lyophilization, which involves freezing… using a stabilizer… which is still in your recom from the puck btw…)

 

[FNG33]

I appreciate you sharing your reasoning. I was curious why you decided it was a reasonable choice for you.

 

[Labcat]

I appreciate you sharing your reasoning. I was curious why you decided it was a reasonable choice for you.

That, and also I am contemplating options, I just got a number if those ProRx vials, someone called it “toilet tirz” this morning. They were $$$ now I don’t feel so great about them. Powders generally cost less in larger mg vials. I wish ignorance is bliss weren’t so true.

 

[Chili777]

Someone might have said that if they sell already reconstituted Tirz. That's because you really have no idea what's in it, both the peptide and the BAC they used and there is almost never a COA. It's also the most expensive way to go, but we already chatted about that. If you really want a US seller, use Nexaph. They sell 10 vial kits of Reta, Tirz, etc., unreconstituted and with a Q Code on the box that links to the Jano COA, so at least you have some idea. It's expensive unless you get a pre-sale but those happen regularly. Then, you can just reconstitute (with Hospira, hopefully) as you go without this freezing business.

 

[Labcat]

Someone might have said that if they sell already reconstituted Tirz. That's because you really have no idea what's in it, both the peptide and the BAC they used and there is almost never a COA. It's also the most expensive way to go, but we already chatted about that. If you really want a US seller, use Nexaph. They sell 10 vial kits of Reta, Tirz, etc., unreconstituted and with a Q Code on the box that links to the Jano COA, so at least you have some idea. It's expensive unless you get a pre-sale but those happen regularly. Then, you can just reconstitute (with Hospira, hopefully) as you go without this freezing business.

I actually was thinking I don’t want another layer of dubiously trustworthy middlemen to both add deliberate shortcuts and inadvertent errors. I will have to suck it up and figure out bitcoin’s intricacies. (I know that sounds harsh but wow the ProRx 483 illustrates what can go badly in the US too. I also have an idea how hard it is to train people to be meticulous and accurate. And then if you get someone awesome they move on so they can be you 🤣, which is awesome but it means that staffing is always variably skilled.)

I heard some things about Nexaph recently having weak tirz that tested poorly, and there was conjecture why. I’m on all these forums and discords suddenly and I can’t keep them straight so I can’t go back and find where that came from so take it with the usual grain of salt.

 

[Labcat]

I actually was thinking I don’t want another layer of dubiously trustworthy middlemen to both add deliberate shortcuts and inadvertent errors. I will have to suck it up and figure out bitcoin’s intricacies. (I know that sounds harsh but wow the ProRx 483 illustrates what can go badly in the US too. I also have an idea how hard it is to train people to be meticulous and accurate. And then if you get someone awesome they move on so they can be you 🤣, which is awesome but it means that staffing is always variably skilled.)

I heard some things about Nexaph recently having weak tirz that tested poorly, and there was conjecture they’re either buying low/failed QA powder or maybe raw and finishing it in their garage. I’m on all these forums and discords suddenly and I can’t keep them straight so I can’t go back and find where that came from so take it with the usual grain of salt.

I spent a couple hours tonight setting up a workspace to filter my 288mg in 16ml of liquid tirz of dubiously trustworthy BUD.

 

[Chili777]

I actually was thinking I don’t want another layer of dubiously trustworthy middlemen to both add deliberate shortcuts and inadvertent errors. I will have to suck it up and figure out bitcoin’s intricacies. (I know that sounds harsh but wow the ProRx 483 illustrates what can go badly in the US too. I also have an idea how hard it is to train people to be meticulous and accurate. And then if you get someone awesome they move on so they can be you 🤣, which is awesome but it means that staffing is always variably skilled.)

I heard some things about Nexaph recently having weak tirz that tested poorly, and there was conjecture why. I’m on all these forums and discords suddenly and I can’t keep them straight so I can’t go back and find where that came from so take it with the usual grain of salt.

The Reta I got was 99.887%. The COA is tied right to the box.Best of luck.

 

[Labcat]

The Reta I got was 99.887%. The COA is tied right to the box.Best of luck.

Very nice. Appreciate your sharing that! I haven’t had reta myself. Have you tried any of that batch yet? Would love to hear your review!

 

[Chili777]

Very nice. Appreciate your sharing that! I haven’t had reta myself. Have you tried any of that batch yet? Would love to hear your review!

That's what I'm using right now. Down 32 lbs in 11 weeks. Eliminated the urge for alcohol, full appetite suppression and I feel great. It's amazing stuff.

 

[Labcat]

That's what I'm using right now. Down 32 lbs in 11 weeks. Eliminated the urge for alcohol, full appetite suppression and I feel great. It's amazing stuff.

Very nice. Congratulations on such a successful journey!

 

[Chili777]

So far, so good. Halfway there.

 

[Grogu]

That, and also I am contemplating options, I just got a number if those ProRx vials, someone called it “toilet tirz” this morning. They were $$$ now I don’t feel so great about them. Powders generally cost less in larger mg vials. I wish ignorance is bliss weren’t so true.

I have some of the "toilet triz" too! Hadn't heard it called that yet, but totally get it. I'm not feeling the love for those vials either (5 of them). My plan is to hold off using the vials and see what happens in the subreddit. And then, I'm thinking I might filter prior to use.

 

[Labcat]

I have some of the "toilet triz" too! Hadn't heard it called that yet, but totally get it. I'm not feeling the love for those vials either (5 of them). My plan is to hold off using the vials and see what happens in the subreddit. And then, I'm thinking I might filter prior to use.

Bacillus is literally butt bacteria. And corynebaster is found in the moist skin folds. Toilet tirz isn’t my phrase, but I like how it covers the basics. My husband suggested Turdzepatide, but I don’t want Eli Lilly to go after him.

I filtered mine. And I took a slightly higher dose than my regular split dose last night. And 15 hrs later I’m feeling nothing, in fact the constant and almost too intense satiety I had for the past 5 weeks went away. This annoys me no end because I had planned to do a quick freeze n thaw of my next split dose and when that worked I was going to toss the whole shebang in the freezer. Now I have to test if it works at all. I might have to just jump to using the PTDS results @Speedy so kindly shared and just toss my other vials in the freezer while I sort this out. (this is compounded and phenol/NaCl/Na phos rather than I assume what was grey BAC but that should matter little.)

I’m not dead so far, so that’s something. Hungry though :-(.

 

[Grogu]

Turdzepatide....

Funny, but not funny.

 

[Labcat]

Turdzepatide....

Funny, but not funny.

What surprises me is that so many people are not worried and aggressively shut down those who are.

 

[Grogu]

What surprises me is that so many people are not worried and aggressively shut down those who are.

I think it's just that people are so "grateful" for not paying $500 a month for brand that they're willing to accept this kind of BS. And maybe they think if people complain too much that compounding will get shut down. I think reddit folks really pushed the FL Pharmacy Board and got Ouisa shut down. Maybe if more people filed complaints with the regulators that might get ProRx to voluntarily recall some of the shipments.

Some people said that Groupon refunded them after they sent in a copy of the 483 report. I've pondered a chargeback with my credit card myself, but would never mention that in the subreddit. Definitely group think there and bullying.

 

[Labcat]

I think it's just that people are so "grateful" for not paying $500 a month for brand that they're willing to accept this kind of BS. And maybe they think if people complain too much that compounding will get shut down. I think reddit folks really pushed the FL Pharmacy Board and got Ouisa shut down. Maybe if more people filed complaints with the regulators that might get ProRx to voluntarily recall some of the shipments.

Some people said that Groupon refunded them after they sent in a copy of the 483 report. I've pondered a chargeback with my credit card myself, but would never mention that in the subreddit. Definitely group think there and bullying.

Makes sense. I did feel the gratitude myself. ($2.77/mg, I now know how much they are raking in though) It’s also mixed with classic sales manipulation - we chose the best place and got a great deal so we become invested in not feeling we were gullible or foolish.

Yah don’t get yourself bullied on there. Everything is stacked against customers, most injustices are rather more arduous to right than they ought to be. Chargeback is a good backup plan, no one is really going to stand up for us. And when this gets swept under the rug, things will get worse.

 

[Labcat]

I think it's just that people are so "grateful" for not paying $500 a month for brand that they're willing to accept this kind of BS. And maybe they think if people complain too much that compounding will get shut down. I think reddit folks really pushed the FL Pharmacy Board and got Ouisa shut down. Maybe if more people filed complaints with the regulators that might get ProRx to voluntarily recall some of the shipments.

Some people said that Groupon refunded them after they sent in a copy of the 483 report. I've pondered a chargeback with my credit card myself, but would never mention that in the subreddit. Definitely group think there and bullying.

Omg also Ouisa - they fucking didn’t have a sterile compounding license. And were just publicly rolling along unimpeded until enough people got pulled in to the mess to complain. 🤦‍♀️. No one is watching out for us.