All right, i searched for freezing tirz, specifically the Jano response but...

[Calm Logic]

Gemini, with a better attitude:

Peptides with Better Freezing Stability

The stability of a reconstituted peptide in a frozen state is primarily determined by its size and amino acid sequence.

Peptide Type	Example (Reconned)	Why It's More Stable Frozen
Dipeptides/Tripeptides	Glutathione (GSH): γ-L-Glutamyl-L-cysteinylglycine (3 residues)	Simplest structure; less prone to complex aggregation upon cryoconcentration. Less susceptible to freezing damage than large proteins.
SS-31 (Elamipretide)	D-Arg-Dmt-Lys-Phe-NH₂ (4 residues)	Its small, linear structure makes it highly stable in frozen aliquots.
BPC-157	Pentadecapeptide (15 residues)	Known to be relatively robust and is frequently stored frozen in aliquots by researchers.

 

[byefatlicia]

with a better attitude:

🤣 🤣 🤣

 

[Gr33dyOctopus]

I think more people don't freeze because the reward doesn't seem enough for the uncertainty. OTOH, as @indolent has mentioned before, freezing is more sterile than refrigeration, which is the best argument for freezing reconned vials:

Yep, would hard agree


Relatively few vendors sell ARA 290. J5 has the kits and BioBombshell has the singles from the same vendor (ZYH, which does retail but mostly bulk sales).

Got it!
ArA 290 check out - biobombshell kits - j5 vials- bulk zyh

 

[Gr33dyOctopus]

Have you seen what Google Gemini says? Basically, you could be injecting Frankenstein GLP, haha. The testing doesn't confirm that, but there could be a touch of freaky in the vial.

I talk to my paid chatgpt all the time, at first it was antipeptide, but I convinced it that these gray market peps are fine and it now suggests and encourages me towards certain ones. Now its on my side !

 

[Gr33dyOctopus]

Have you been drinking? 🤣🤣🤣😇

Following you too, you fucking maniac!!!

 

[Gr33dyOctopus]

I’m doing it. Recon a R60 into 3x R20 vials and freeze 2. If it affects the product I figure I’ll feel it

Thats why your a goddamn awesome lab rat, and why everyone loves you. 😃😍😂

 

[byefatlicia]

My rats don't wanna be guinea pigs. They're more like scaredy cats.

 

[YoYoFat]

PTDS has data on thawing reconstituted tirzepatide multiple times, and it seems like there was no loss in potency. So I would readily consider freezing reconstituted tirzepatide for later use safe.

I don’t think this is accurate. There’s a chart that shows significant degradation with repeated thaw cycles.

 

[Nathanologist]

I don’t think this is accurate. There’s a chart that shows significant degradation with repeated thaw cycles.

I’m not yet on their server… but this is the exact opposite of what I’ve seen posted across multiple communities regarding this test they did. I reserve judgment, however, until I’ve actually seen their results on their server. Working on it.

 

[Gr33dyOctopus]

My rats don't wanna be guinea pigs. They're more like scaredy cats.

Mine are uber trusting that im not going to Kill or maim them, and as brave as lions.

 

[94brian49]

Interesting topic, parking here just to see if anybody post any result in the future.
For me i prefer them in the white powder form, who knows maybe i would just snort them up 😀

 

[Foggy-Hollow]

Someone was asking about freezing recon on Read It, I forget which pep sub (I’m on a few). Pretty sure it was in regards to tirz, maybe the compounded tirzepatide area.

The vendor link they had stated -80C for reconned. I asked that OP how they were planning on getting to -80C and I didn’t get an answer. 🤷‍♀️ surprised picachu

Jano seems to be putting interesting data behind storage and handling of peptides.

I’m doing it. Recon a R60 into 3x R20 vials and freeze 2. If it affects the product I figure I’ll feel it

Or maybe not feel it? 🙃😆

Seems like this family of peptides is pretty robust. Not sure how that extends to other molecules. My bet is that it’ll be fine. HOWEVER im not a place yet where I’d pin it myself. 😆 I expect a full report!

 

[Gr33dyOctopus]

Someone was asking about freezing recon on Read It, I forget which pep sub (I’m on a few). Pretty sure it was in regards to tirz, maybe the compounded tirzepatide area.

The vendor link they had stated -80C for reconned. I asked that OP how they were planning on getting to -80C and I didn’t get an answer. 🤷‍♀️ surprised picachu

Jano seems to be putting interesting data behind storage and handling of peptides.



Or maybe not feel it? 🙃😆

Seems like this family of peptides is pretty robust. Not sure how that extends to other molecules. My bet is that it’ll be fine. HOWEVER im not a place yet where I’d pin it myself. 😆 I expect a full report!

Mmmmm, I am also a member of r/retatrutide and a ton of guys freeze thiers with few ill effects. Also on r/tirzepatidecompounds, those fellas constantly freeze vials with 3 or 10 mo th BUDs in them. Lol, I just want the consensus of this board and its pretty much what I had already known.

 

[Foggy-Hollow]

Take lots and lots of notes. I know you’re excited… I thought I was taking reasonable notes until I realized I was becoming a pincushion in the two areas I liked to pin. 6 pokes per week, Body was asking to change things up. Got an ISR and could t remember fine details from a week ago, two weeks ago.

If you have a side effect you don’t like you’ll want to trace it back. Whether it’s ISR, puking, whatever.

 

[Gr33dyOctopus]

Take lots and lots of notes. I know you’re excited… I thought I was taking reasonable notes until I realized I was becoming a pincushion in the two areas I liked to pin. 6 pokes per week, Body was asking to change things up. Got an ISR and could t remember fine details from a week ago, two weeks ago.

If you have a side effect you don’t like you’ll want to trace it back. Whether it’s ISR, puking, whatever.

Good advice, im only pinning 2x per week now, but thats going to change very soon. Im teying to make sure that I dont fuck things up to badly. Anything that messes with my heartrate and blood sugar in particular. And I am excited! Haha most of my hobbies and interests along the way have fallen off as my weight has increased

 

[YoYoFat]

I’m not yet on their server… but this is the exact opposite of what I’ve seen posted across multiple communities regarding this test they did. I reserve judgment, however, until I’ve actually seen their results on their server. Working on it.

Having not seen the test results yourself, and not being in the server to see related discussions, it would appear that what you’re relying on is rumor.

 

[Labcat]

Didn't really see it. But know it exists. Apologize in advance if im posting questions that have been answered, just trying to get a handle on all of this.

Ok, my question is, freezing reconstituted tirz. I have this 30ml of bac water and it would be cool to use the whole thing on ten vials. Calm Logic, or Indolent, or someone else thats been helping me along here mentioned freezing bottles of tirz 10 at a time.

That maybe it was Jano that said we can freeze this shit as long as there isnt to many thaw cycles, that even after... a year or two reconstituted tirz is frozen, when thawed there is barely a poentcy loss. Is this true for others? Does anyone have a link to Jano talking about this subject?

As a bonus question has anyone tried KPV? Thoughts on it?

Thanks in advance fuckers!

I’m also searching for some hard data on that same question, but meanwhile, I recommend that you freeze that BAC vial to keep it longer than its own 30day cycle. ETA but not lower than 4.6F, it’s melting point. (Any chemists think this is unreasonable?)
I know the instructions say to keep it in at room temp, but maybe that is a problem with condensation getting pushed into the vial? Also lab freezers are -20C, which is below 4.6F.
And additional good move is to minimize pushing extra air into the vial since oxygen degrades benzyl alcohol. Benzyl alcohol itself degrades over time with alkaline (high) pH, oxygen, and exposure to its ozidation product, benzaldehyde, is my understanding, of course keeping an eye out for data about that as well.

I added all this because I’m trying in my own head to figure out what happens to the benzyl alcohol after you froze the peptide/BAC solution, perhaps gently make sure the benzyl alchol redissolves and gets mixed back in also if you are going to keep it in the fridge after and are relying on its bacteriostatic property.

As always, anyone with a mechanism that contradicts any of what I’m thinking, please do bring it up

 

[byefatlicia]

I’m also searching for some hard data on that same question, but meanwhile, I recommend that you freeze that BAC vial to keep it longer than its own 30day cycle. ETA but not lower than 4.6F, it’s melting point. (Any chemists think this is unreasonable?)
I know the instructions say to keep it in at room temp, but maybe that is a problem with condensation getting pushed into the vial? Also lab freezers are -20C, which is below 4.6F.
And additional good move is to minimize pushing extra air into the vial since oxygen degrades benzyl alcohol. Benzyl alcohol itself degrades over time with alkaline (high) pH, oxygen, and exposure to its ozidation product, benzaldehyde, is my understanding, of course keeping an eye out for data about that as well.

I added all this because I’m trying in my own head to figure out what happens to the benzyl alcohol after you froze the peptide/BAC solution, perhaps gently make sure the benzyl alchol redissolves and gets mixed back in also if you are going to keep it in the fridge after and are relying on its bacteriostatic property.

As always, anyone with a mechanism that contradicts any of what I’m thinking, please do bring it up

From Google Gemini
Recommended Storage

• Unopened Vials: Store at room temperature, typically between 20 to 25°C (68 to 77°F), away from light, heat, and moisture.
• Opened Vials: After opening and puncturing the vial, it should be refrigerated at 2–8°C (36–46°F) to help maintain the preservative's effectiveness and minimize contamination risk. It should be discarded 28 days after the first use, as the benzyl alcohol preservative loses potency over time.

Important Considerations:

• Always check and follow the specific storage instructions provided by the manufacturer of the bacteriostatic water or the medication you are reconstituting.
• Freezing water does not kill all bacteria; some can survive freezing temperatures. The 0.9% benzyl alcohol in bacteriostatic water works by inhibiting bacterial growth, not by killing existing bacteria

In addition: Hospira recommends storing their BAC at room temperature out of light. until opened and then refrigerate.

 

[Labcat]

From Google Gemini
Recommended Storage

• Unopened Vials: Store at room temperature, typically between 20 to 25°C (68 to 77°F), away from light, heat, and moisture.
• Opened Vials: After opening and puncturing the vial, it should be refrigerated at 2–8°C (36–46°F) to help maintain the preservative's effectiveness and minimize contamination risk. It should be discarded 28 days after the first use, as the benzyl alcohol preservative loses potency over time.

Important Considerations:

• Always check and follow the specific storage instructions provided by the manufacturer of the bacteriostatic water or the medication you are reconstituting.
• Freezing water does not kill all bacteria; some can survive freezing temperatures. The 0.9% benzyl alcohol in bacteriostatic water works by inhibiting bacterial growth, not by killing existing bacteria

In addition: Hospira recommends storing their BAC at room temperature out of light. until opened and then refrigerate.

I think the first part for unopened vials is not for chemical stability, but practicality, so that the cost of cold storage doesn’t become more than the cost of the product.

And the second is so that the cold reduces bacterial replication, esp if the bacteria is less susceptible to benzyl alcohol. How good is it, I wondered… it’s -static so they din’t measure it the same way, but if you use 1% as a cleaner, it yuelds 3 log reduction in 5 minutes, so mildly suppressive, and affects some organisms less than others. I’m not aware that any medical office I’ve ever been in stores their BAC in the fridge (though I haven’t been in many, lol, I’m a curious sort when the staff if also friendly).

I’ll also note that even refrigerator temps do not slow down all bacterial growth, for instance Listeria thrives.

I’m not insisting anyone do this of course, just trying to pull apart the science from the practice. Also how can water cost $15/30ml lol. (Sterile water by itself is not much cheaper, either.)

Does anyone happen to know what pH lyophilized tirz will reconstitute at? Or what pH compounding pharmacies are using?

 

[byefatlicia]

I think the first part for unopened vials is not for chemical stability, but practicality, so that the cost of cold storage doesn’t become more than the cost of the product.

This was part of the Gemini quote. I guess I missed copying it.

"You should not freeze bacteriostatic water. Freezing can damage the solution and affect its efficacy or the stability of any medications mixed with it"

I'm not qualified to give answers as to the whys or chemistry. I only know what many have taught me was safest.

 

[Labcat]

This was part of the Gemini quote. I guess I missed copying it.

"You should not freeze bacteriostatic water. Freezing can damage the solution and affect its efficacy or the stability of any medications mixed with it"

I'm not qualified to give answers as to the whys or chemistry. I only know what many have taught me was safest.

Gemini is an AI? Did it say where that quote comes from? I think a lot of us are here because what we were taught may not be as strongly supported as we’d believed. (Grey is bad, compounding is unsafe, you can’t mix and dose yourself, etc.)

Are you in the camp then of people who don’t freeze their peptide solution, and is that
because you recon with bacterioststic water which you don’t freeze? I just think there is a lot of evidence that things work that are not “supposed” to and I’m interested in sorting it out. (I understand not everyone is.)

 

[byefatlicia]

Are you in the camp then of people who don’t freeze their peptide solution, and is that
because you recon with bacterioststic water which you don’t freeze? I just think there is a lot of evidence that things work that are not “supposed” to and I’m interested in sorting it out. (I understand not everyone is.)

I am in that camp. I tossed several bottles of Hospira Bac after accidentally freezing them. Again, I'm not that person who pretends to know why it's right. But others have made strong cases for what I adhere to.
It's really confusing to see folks arguing what I thought was gospel. I also welcome more dialog and more evidence. But for now, my rats don't wanna be guinea pigs.

 

[Calm Logic]

Related thread: https://glp1forum.com/threads/freezing-bac-water.1540/

 

[Labcat]

I am in that camp. I tossed several bottles of Hospira Bac after accidentally freezing them. Again, I'm not that person who pretends to know why it's right. But others have made strong cases for what I adhere to.
It's really confusing to see folks arguing what I thought was gospel. I also welcome more dialog and more evidence. But for now, my rats don't wanna be guinea pigs.

It’s gospel that we shouldn’t be injecting grey substances at all, so I’m equally curious about everything, trying to aort out science from rules that optimized other things, and trying to either find the reason something is being touted or reasons why it shoud not be. Great to have a place where discussions flourish in a contest of people also trying to maintain safe practices.,

Benzyl alcohol is a small molecule, and I’m curious as to whether it is enough to destabilize a peptide if you freeze to below it’s freezing point (have a thermometer in your fridge/freezer). I’ve seen data where only high concentration (2.2%) destabilized the peptide when you froze it. But also that was a very fragile protein, not like tirz is reputed to be.

One could just try it, or keep the freeze light, or one could reconstitute with water then freeze.

I’m super curious whether anyone has experience with a tirz or other peptide with 0.9% benzyl alcohol that they froze and used?

 

[Labcat]

Related thread: https://glp1forum.com/threads/freezing-bac-water.1540/

Thank you for this link, good info there and some discussion! Appreciate it 🙏🏼

 

[Labcat]

Mmmmm, I am also a member of r/retatrutide and a ton of guys freeze thiers with few ill effects. Also on r/tirzepatidecompounds, those fellas constantly freeze vials with 3 or 10 mo th BUDs in them. Lol, I just want the consensus of this board and its pretty much what I had already known.

So is the consensus of this board to freeze or not freeze? It’s good to know that the reta guys freeze just fine.

In fact if you go to the Sigma-Aldritch (and related) sites, they generally instruct people to freeze delicate peptide solutions. (And some cool instructions for identifying which amino acids tend to destabilize in certain ways). It makes me think that the default is more stable than unstable, though some molecules do hate freezing.

 

[Calm Logic]

Most here don't freeze after recon. But most here don't filter either (and neither do I anymore).

Of course, it often comes down to risk tolerance, which generally increases over time.

 

[Labcat]

Most don't freeze after recon.

I probably wouldn’t unless it was large vials supplying longer than a month or two. Which is def something I’d do. My issue at hand is that I have a compounded order finally coming but it seems they are sending out rather older stock after showing new mfg dates, so I’m thinking it might be better to freeze for potency. They use phenol, not BAC though.

 

[Calm Logic]

To me, the best argument for freezing (reconned or lyophilized) is sterility, rather than potency. Tirz will be potent for a very long time, no matter what.