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[AcadiaPeptides]

You wanted 4mm, not 13mm?

yes, I won't be doing more than 3ml vials, maybe 4ml at a time

 

[AcadiaPeptides]

I was filtering a couple of days ago and got lost in my steps, I'm pretty absent minded these days, so I decided to write these down for my own use. I wrote these based on the video I posted from Peptide test. It seems like a lot of needles, but needles are so inexpensive, it makes sense to discard when they are man handled. I'm sure I'll pick up speed the more and more I filter and probably won't need a checklist eventually.


Step 0. Thoroughly wipe down the work surface with 70% isopropyl alcohol (IPA) and allow to fully dry before placing any materials. Wipe all vial stoppers and cartridge septa with 70% IPA and allow to dry prior to use.
Step 1. Reconstitute lyophilized peptide per standard protocol.
Step 2. Detach the reconstitution needle from the luer-lock syringe; safely discard the needle.
Step 3. Insert a sterile venting needle into the destination vial or cartridge to equalize pressure during transfer. (Optional: fit the venting needle with a 0.22 µm filter to maintain sterility of the headspace prior to insertion if so desired.)
Step 4. Attach a fresh drawing needle to the luer-lock syringe.
Step 5. Draw the full volume of reconstituted peptide into the syringe.
Step 6. Detach the drawing needle from the luer-lock syringe; safely discard the needle.
Step 7. Attach a sterile 0.22 µm syringe filter directly to the luer-lock tip of the syringe. Attach a sterile needle to the outlet port of the filter.
Step 8. Pierce the septum of the destination vessel with the outlet needle and express the peptide solution through the filter into the destination vessel.

Optional: Filter Recovery Flush
(Recovers the dead volume / hold-up volume retained within the filter housing — typically 0.1–0.2 mL (10–20 units on a U-100 syringe), depending on filter size.)

Step 9. With the venting needle still seated in the destination vessel, carefully disconnect the luer-lock syringe from the inlet of the filter, leaving the filter and outlet needle in place in the septum.
Step 10. Attach a fresh drawing needle to the now-empty luer-lock syringe.
Step 11. Draw an appropriate volume of bacteriostatic water into the syringe — typically equal to or slightly greater than the filter's rated hold-up volume (e.g., 0.1–0.2 mL / 10–20 units on a U-100 syringe).
Step 12. Detach the drawing needle from the luer-lock syringe; safely discard the needle.
Step 13. Reconnect the luer-lock syringe (containing bacteriostatic water) to the inlet port of the filter assembly from Step 9.
Step 14. Slowly express the bacteriostatic water through the filter to flush retained peptide into the destination vessel.
Step 15. Remove the filter assembly and venting needle. Cap or seal the destination vessel. Gently swirl or invert to mix the flush volume with the filtered peptide. Label with peptide name, concentration (adjusted for flush dilution if relevant), reconstitution date, and diluent used.

Fantastic write up. I just copied it to Word, saved, printed it out, and taped it to my workbench. Thank you SOP brother!

 

[myboysherman]

I burst out a 4mm filter recently and I think I’m going to skip filtering in the future. There’s a jk interview where he talks about endotoxin risk and it applies to bacteria as well. “janoshik shocking” it’s a good interview.

Or I will switch to 13mm and purge with a reserved amount of recon solution, but I really don’t think it’s worth it.

Edit: Getting past the auto filter was tricky here. The hold up was unexpected and silly. 34:33 is the star on the vid.

 

[Chili777]

I burst out a 4mm filter recently and I think I’m going to skip filtering in the future. There’s a jk interview where he talks about endotoxin risk and it applies to bacteria as well. “janoshik shocking” it’s a good interview.

Or I will switch to 13mm and purge with a reserved amount of recon solution, but I really don’t think it’s worth it.

Edit: Getting past the auto filter was tricky here. The hold up was unexpected and silly. 34:33 is the star on the vid.

They can do that if you use too much force. It should take about 30 seconds to purge the syringe with 2 mL BAC. Your brand may also be faulty. I order mine from Peptidetest and haven't had a bad one, yet.

And for 4 mm, I just do an air purge by detaching the filter, pull 2 ml of air in, reattach the filter, and push it through. I call it a day after that.

 

[CNCCurrency]

I was filtering a couple of days ago and got lost in my steps, I'm pretty absent minded these days, so I decided to write these down for my own use. I wrote these based on the video I posted from Peptide test. It seems like a lot of needles, but needles are so inexpensive, it makes sense to discard when they are man handled. I'm sure I'll pick up speed the more and more I filter and probably won't need a checklist eventually.


Step 0. Thoroughly wipe down the work surface with 70% isopropyl alcohol (IPA) and allow to fully dry before placing any materials. Wipe all vial stoppers and cartridge septa with 70% IPA and allow to dry prior to use.
Step 1. Reconstitute lyophilized peptide per standard protocol.
Step 2. Detach the reconstitution needle from the luer-lock syringe; safely discard the needle.
Step 3. Insert a sterile venting needle into the destination vial or cartridge to equalize pressure during transfer. (Optional: fit the venting needle with a 0.22 µm filter to maintain sterility of the headspace prior to insertion if so desired.)
Step 4. Attach a fresh drawing needle to the luer-lock syringe.
Step 5. Draw the full volume of reconstituted peptide into the syringe.
Step 6. Detach the drawing needle from the luer-lock syringe; safely discard the needle.
Step 7. Attach a sterile 0.22 µm syringe filter directly to the luer-lock tip of the syringe. Attach a sterile needle to the outlet port of the filter.
Step 8. Pierce the septum of the destination vessel with the outlet needle and express the peptide solution through the filter into the destination vessel.

Optional: Filter Recovery Flush
(Recovers the dead volume / hold-up volume retained within the filter housing — typically 0.1–0.2 mL (10–20 units on a U-100 syringe), depending on filter size.)

Step 9. With the venting needle still seated in the destination vessel, carefully disconnect the luer-lock syringe from the inlet of the filter, leaving the filter and outlet needle in place in the septum.
Step 10. Attach a fresh drawing needle to the now-empty luer-lock syringe.
Step 11. Draw an appropriate volume of bacteriostatic water into the syringe — typically equal to or slightly greater than the filter's rated hold-up volume (e.g., 0.1–0.2 mL / 10–20 units on a U-100 syringe).
Step 12. Detach the drawing needle from the luer-lock syringe; safely discard the needle.
Step 13. Reconnect the luer-lock syringe (containing bacteriostatic water) to the inlet port of the filter assembly from Step 9.
Step 14. Slowly express the bacteriostatic water through the filter to flush retained peptide into the destination vessel.
Step 15. Remove the filter assembly and venting needle. Cap or seal the destination vessel. Gently swirl or invert to mix the flush volume with the filtered peptide. Label with peptide name, concentration (adjusted for flush dilution if relevant), reconstitution date, and diluent used.

 

[myboysherman]

Thank you, chili777. I used a lot of force and it was taking several minutes for every ten units.

Had to edit a bit. This probation period is challenging. Lol.

 

[Chili777]

Thank you, chili777. I used a lot of force and it was taking several minutes for every ten units.

Had to edit a bit. This probation period is challenging. Lol.

If you had to press that hard, it was a dud filter. If you don't see a flow from the needle within 5 seconds or so, it's bad. It happens with some brands more than others.

 

[BNLFL]

yes, I won't be doing more than 3ml vials, maybe 4ml at a time

I thought I read 4mm is only good or recommended for 1ml.

 

[Chili777]

I thought I read 4mm is only good or recommended for 1ml.

Depends on your comfort level. I use 4 mm for 3 mL and less, 13 mm for anything over that. It's almost like a BAC discussion. People have their own opinions and stick to them.

 

[fremt52958]

Same. I filter everything because why not screw a filter on there when I'm moving it to a new home anyway. I use a 4mm filter for everything but KLOW, and I do not bother myself about the 0.5 mg max I might lose in the holdup. I got more.

I know this is an older post. But why do you not filter KLOW? Or do you use a larger filter?

 

[randompersonrandom]

I know this is an older post. But why do you not filter KLOW? Or do you use a larger filter?

I use a 13mm filter. I usually recon it weak because of my tendancy toward bruising with it, so one vial of the stuff I have ends up being 12ml, and that's too much to put through a 4 mm.

 

[randompersonrandom]

I was filtering a couple of days ago and got lost in my steps, I'm pretty absent minded these days, so I decided to write these down for my own use. I wrote these based on the video I posted from Peptide test. It seems like a lot of needles, but needles are so inexpensive, it makes sense to discard when they are man handled. I'm sure I'll pick up speed the more and more I filter and probably won't need a checklist eventually.


Step 0. Thoroughly wipe down the work surface with 70% isopropyl alcohol (IPA) and allow to fully dry before placing any materials. Wipe all vial stoppers and cartridge septa with 70% IPA and allow to dry prior to use.
Step 1. Reconstitute lyophilized peptide per standard protocol.
Step 2. Detach the reconstitution needle from the luer-lock syringe; safely discard the needle.
Step 3. Insert a sterile venting needle into the destination vial or cartridge to equalize pressure during transfer. (Optional: fit the venting needle with a 0.22 µm filter to maintain sterility of the headspace prior to insertion if so desired.)
Step 4. Attach a fresh drawing needle to the luer-lock syringe.
Step 5. Draw the full volume of reconstituted peptide into the syringe.
Step 6. Detach the drawing needle from the luer-lock syringe; safely discard the needle.
Step 7. Attach a sterile 0.22 µm syringe filter directly to the luer-lock tip of the syringe. Attach a sterile needle to the outlet port of the filter.
Step 8. Pierce the septum of the destination vessel with the outlet needle and express the peptide solution through the filter into the destination vessel.

Optional: Filter Recovery Flush
(Recovers the dead volume / hold-up volume retained within the filter housing — typically 0.1–0.2 mL (10–20 units on a U-100 syringe), depending on filter size.)

Step 9. With the venting needle still seated in the destination vessel, carefully disconnect the luer-lock syringe from the inlet of the filter, leaving the filter and outlet needle in place in the septum.
Step 10. Attach a fresh drawing needle to the now-empty luer-lock syringe.
Step 11. Draw an appropriate volume of bacteriostatic water into the syringe — typically equal to or slightly greater than the filter's rated hold-up volume (e.g., 0.1–0.2 mL / 10–20 units on a U-100 syringe).
Step 12. Detach the drawing needle from the luer-lock syringe; safely discard the needle.
Step 13. Reconnect the luer-lock syringe (containing bacteriostatic water) to the inlet port of the filter assembly from Step 9.
Step 14. Slowly express the bacteriostatic water through the filter to flush retained peptide into the destination vessel.
Step 15. Remove the filter assembly and venting needle. Cap or seal the destination vessel. Gently swirl or invert to mix the flush volume with the filtered peptide. Label with peptide name, concentration (adjusted for flush dilution if relevant), reconstitution date, and diluent used.

oh, thanks for this! My mom is irritated because steps 1-8 are still not quite clicking for her, and I'm on video talking her through it, but she moves fast and HATES being talked at (which I can't judge too hard; I am the same way, but mom, you wanted me here, this is the job you asked me to do), so I'm not always quick enough to stop her from the mistake she's about to make, and it's hard for both of us when I have to say 'Hold up. Ok, so you just put the long needle back onto that syringe with the filter, which means technically you just contaminated your filter. So you have a choice now to either JUST switch the needle and say 'fuck it, it's probably fine,' and don't do that again, or take off the filter and the needle, grab a new filter, and grab a fresh needle, which is what I'd do if it were me."

That happened on Saturday, but she got bored of the sound of my voice, pulled the needle off but not the filter, and started to add another filter onto the first filter without realizing it. So I had to say "Ok, hold up. You're trying to shove a fresh filter on the contaminated filter now, which means technically you just contaminated your second filter too" and that was ALSO a pretty tough moment for both of us.

I think if she's got this list and can follow it like a recipe while she's working, it'll solve both the "I feel more comfortable having randompersonrandom on video watching while I do this" and "randompersonrandom cannot talk fast enough to finish her sentence before I've turned her out because I'm bored of listening, but I have the steps right here and I can read them faster than she can talk."

Which, again, I cannot even slightly judge because I'm much the same.

 

[LabRatBarbie]

I use a 13mm filter. I usually recon it weak because of my tendancy toward bruising with it, so one vial of the stuff I have ends up being 12ml, and that's too much to put through a 4 mm.

I could be wrong but I read in a post that if you recon with 1ml you can push it through the 4mm filter. Then add the remaining bac (as long as its the H bac) after filtration.

 

[randompersonrandom]

I could be wrong but I read in a post that if you recon with 1ml you can push it through the 4mm filter. Then add the remaining bac (as long as its the H bac) after filtration.

I could, but I don't want to go back in my bac with a luer lock that's had something else in it. Same reason I don't mess with flushing. When I do the weak recon, I do 9ml of bac into the sterile vial first, then 3ml into the pep. I know the chances of contamination are small if I change out the needle, and nonexistent if I burn a whole new luer lock, but I don't actually mind using the 13mm for the KLOW; they're cheaper than the 4mm's, and I'm not bothered by the holdback.

 

[thorien]

Is peptide test a good place to get a starter kit? Thinking of ordering one.

 

[V6R3W]

Is peptide test a good place to get a starter kit? Thinking of ordering one.

Yes. underground supply is also good; that is where I got mine.

 

[thorien]

Thanks I'll get one . Shouldn't be any issues putting any of this in hold luggage when flying right ?

 

[HereKittyKitty]

Thanks I'll get one . Shouldn't be any issues putting any of this in hold luggage when flying right ?

Right.

Injectionshop.com also has starter kits and a fab online calculator: calc.injectionshop.com

 

[desinr-gal]

I didn't realize that the stopppers have to pop for the sterilization to work. I was thinking that the overall heat and pressure would pentrate the cartridge. Thanks for the heads up. I see over on Peppys that some folk use UV light. What you think about that method?

I saw one for sale on amazon I was waiting for opinions

 

[desinr-gal]

Fantastic write up. I just copied it to Word, saved, printed it out, and taped it to my workbench. Thank you SOP brother!

It's great that you did, because peptidetest.com has removed the videos from their website!

 

[desinr-gal]

I thought I read 4mm is only good or recommended for 1ml.

Peptide test did several videos in which he pushed at least 4ml through but did burst it at the end.

 

[BinaryPinecone]

I had also heard 4mm was best. How do these work for you? Are they the same and just afresh to the luer lock?

I actually think that 13mm are more versatile as others have told me. The 4mm filter gets clogged very easily and pressing harder to make it go through just rips the membrane eventually. I had no trouble with reta, but I just tried filtering a cjc1295+ipa mix and it took legitimately so long that I just rage quit mid way and had to use my way too big 44mm filter (I don't have 13mms). So really get 13mms, but 4mms can be useful if you know that the peptide that you have won't block the pores and make the filtering super annoying. The 4mm filter also made filtering 100mg ghk-cu super super annoying and tedious. And yes they work with luer locks.

 

[desinr-gal]

I actually think that 13mm are more versatile as others have told me. The 4mm filter gets clogged very easily and pressing harder to make it go through just rips the membrane eventually. I had no trouble with reta, but I just tried filtering a cjc1295+ipa mix and it took legitimately so long that I just rage quit mid way and had to use my way too big 44mm filter (I don't have 13mms). So really get 13mms, but 4mms can be useful if you know that the peptide that you have won't block the pores and make the filtering super annoying. The 4mm filter also made filtering 100mg ghk-cu super super annoying and tedious. And yes they work with luer locks.

Yeah I am thinking of using those instead. The toss rate for the Tisch 4mm is crazy high. I didnt like the idea of wasting more solution in them, and more plastic..but since 13mm can handle more flushing, and without clogging, failing, I think it resolves that issue. It's what @shadesofgreymarket recommended, I should have listened.

 

[myboysherman]

I actually think that 13mm are more versatile as others have told me. The 4mm filter gets clogged very easily and pressing harder to make it go through just rips the membrane eventually. I had no trouble with reta, but I just tried filtering a cjc1295+ipa mix and it took legitimately so long that I just rage quit mid way and had to use my way too big 44mm filter (I don't have 13mms). So really get 13mms, but 4mms can be useful if you know that the peptide that you have won't block the pores and make the filtering super annoying. The 4mm filter also made filtering 100mg ghk-cu super super annoying and tedious. And yes they work with luer locks.

The only time I have used 4mm successfully was when I added some 60 day old BAC to a vial and figured it wouldn't hurt to filter it. On three other occasions, I had one complete filtering that took several minutes at fairly high pressure, one almost immediate clog followed by a burst membrane, and one "that's it, I'm out" failure. 13mm has been fine. I have been pushing 20 units or more to purge them post filtering, but air would probably be fine too.

Maybe I had bad filters, but they were from a reputable supplier.

 

[BinaryPinecone]

Maybe I had bad filters, but they were from a reputable supplier.

Nah that's been my experience with half of the peptides that I've tried. Some just go through fine and others not so much.