Putting Air in the syringe and into your vial.

This is one of dozens of reasons I went to pens really early on, and was glad my mom came to the same conclusion for her own stuff. No air, and no vacuum to fight.
 
To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill
I only recon in front of my flow hood, so all air going into a vial is clean.
I use pens as well.
 

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