joearimathea
Recently Joined
I see some discussion about filtering peptides. If they are 99% pure, then what are you filtering out? One of my worries is introducing a contaminant.
To filter or not to filter, that is the question
- Thread starter joearimathea
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99 percent purity doesn't mean that what you see in your lyophilized vial is 99 percent active ingredient.
Purity is a very misunderstood metric in the market.
I still don't filter.... infections from SQ injections are pretty rare for most people.
Purity is a very misunderstood metric in the market.
I still don't filter.... infections from SQ injections are pretty rare for most people.
chmuse
Public Nuisance 🦜🐧🦅🦚🦃🦢🐓🦆🦉
I don't filter because I didn't see a reason for a subq injections. That being said, I'm now leaning towards 'the stuff to do it is cheap anyway' and plan to buy supplies to do so, if only because I want to learn.
joearimathea
Recently Joined
So do you filter each injection or filter into a new sterile vial?
HPLC purity tests tell you the purity of whatever they are testing for, not the composition of what is in the vial. That puck you see is almost entirely excipient, generally mannitol.
A purity test tells you absolutely nothing about contaminants of most kinds, be it bacteria, mold, heavy metals, glass shards, etc.
raw_oyster_eater
lemon bottle brewer
they poke holes in the fat cells. good stuff.
Healing injuries requires energy expenditure. Raises your metabolism!
GimmeABreak
Rabbit-hole Explorer
I filter anything that is going into a cartridge (because it easy to do while filling it) or any pep I plan to use for greater than a few days.
10mg MOTs-C, gone in a day or two, I don't bother. Reta, Tirz even my dirty-ass lab originated Ousia compound Tirz gets filtered every time.
10mg MOTs-C, gone in a day or two, I don't bother. Reta, Tirz even my dirty-ass lab originated Ousia compound Tirz gets filtered every time.
TooBigtoFail
GLP-1 Novice
Was not filtering. Then realized cheap Amazon bac water was risky. I began to filter using questionable EO needles, attached to questionable filter, to EO syringe made in some Asian country, to filter Bac water that could be tap water, to do recon. Was creating a much greater risk. I did buy a box of EO sterile vials but wondered how sterile can cheap vials be? The address is US but is shipped from China!!! Now use Hospira into lyophilized vial directly and leave a few drops in the vial on the last draw. Minimal manipulation and $$. Medical grade supplies are not cheap.
nonyabizznez
GLP-1 Specialist
I'm on anti rejection medication so low immunity. I filter each time I reconstitute a vial so maybe twice a month. I found a new AliExpress source for 4mm filters so my $3.20 formerly going to Peptide Test is now $1.80 for the 2 filters. Either filter expense is more of a "why not?" My partner sees me taking extra precautions and doesn't question this stuff I'm injecting came from the same place as his ramen noodles.
scarywood75
Scarlett Johansson
I filter, cuz it's cheap and quick to do, but it's important to know that it does not filter out all pathogens nor replace other precautions.
Bigger pathogens aren't more dangerous, size actually doesn't matter here lol.
So don't assume that you get a sterile solution when filtering and take other precautions along the way and you shall be good.
Bigger pathogens aren't more dangerous, size actually doesn't matter here lol.
So don't assume that you get a sterile solution when filtering and take other precautions along the way and you shall be good.
GimmeABreak
Rabbit-hole Explorer
This is great to get the chunks out of your peps.
Every "purity" test you see of UGL peptides is conducted AFTER being filtered with a .22um syringe filter. Contaminants larger than that, which are common, are removed first.
Unless you're filtering first, you're not injecting what you see in the test result, but the "raw" version with some unknown amount of contamination, consisting of glass delimitation shards, rubber particulates, bacteria, peptide aggregates, etc.
Unless you're filtering first, you're not injecting what you see in the test result, but the "raw" version with some unknown amount of contamination, consisting of glass delimitation shards, rubber particulates, bacteria, peptide aggregates, etc.
keangkong
GLP-1 Specialist
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Anti rejection medication? Does that mean when I ask a girl out, she’ll say yes? If so, where do I get some?
What you speak of is just money. Lol
nonyabizznez
GLP-1 Specialist
I don't know...never tried. 🏳️🌈
penewbie
GLP-1 Specialist
Whoa, that's crazy, I had no idea.
It doesn't seem to be a commonly understood limitation of the type of testing we have access to.
The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for.
The inorganic particulates in cheap Chinese vials not certified to meet USP (the standards setting organization the FDA follows) particulate standards alone warrants filtering.
Injected glass and rubber particulates have been found lodged in many organs during autopsies. They create "micro embolisms" and/or chronic inflammation. With no natural means for glass or rubber to be broken down or removed, they accumulate with each injection.
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penewbie
GLP-1 Specialist
To be fair I always filter.
I do it because I listened to Ghoul on meso, who is a total fear mongering loudmouth, but he does make good points every now and again.
I'm not worried about bacteria in subq shots, but he showed that immunogenicity could be a realistic concern, so I filter.
It's a "nice" surprise to now be able to add another reason to my filtering, strengthening my decision.
Hi and welcome, brand new member.
I can appreciate that "The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for."
But are you stating here that JANOSHIK TESTS of GLPs etc are "conducted AFTER being filtered with a .22um syringe filter"?
... which would mean that every time we confirm our product purchases with a Jano test, we are not actually learning what's in our own (unfiltered) vials?
And that we are not actually using what we think we are UNLESS we filter it that way?
Because I have never, ever come across that piece of information.
Nor do I filter.
All the testing companies have to do this to protect their equipment. Water soluble stuff does not get trapped in the filter so theres no loss of the peptides.
Also edited to add: they don't test for what's in the vial. They only test for what you specifically ask for. Tirz test will only test for tirz quantity and mass. It won't tell you if there's anything else in the vial.
THANK YOU for that screenshot! I just wanted confirmation that JANO did it.
Appreciate it, and am astounded you were able to grab it so fast ... wait a minute — are you the Mr. Smith from The Matrix by any chance? 🤔
Thanks, Vicki!
(I had known your second paragraph stuff, but just wanted to confirm Janoshik was counted among "ALL" the testing companies doing the first paragraph stuff, lol)
No, but you might know me from my infatuation with Ning Yang Bing on QUAL.
I took that screenshot from SWISS under lab chat with Jano and TP.
hahah I would tell you hands off Ning, mister, but now I'm kinda infatuated with you.
